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We narrowed to 1,015 results for: LEA

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Showing: 31 - 60 of 1015 results

  1. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... and the systemic effects of Cre toxicity. Leakiness Leaky expression, where the gene of interest is expressed...Learn about Cre-Lox and other site-specific recombinase systems, how to use them to modify the genome... from a mycobacteriophage, are serine SSRs that cleave all four DNA strands at once. They can mediate ...when a recombinase enzyme is present. Read on to learn about the different ways that Cre-lox and other ... Expression may cause repetitive recombination leading to genomic instability and cell death. It is important...populations. Read our blog post about how to minimize leakiness in experiments using AAV vectors. Highlighted ...

  2. CRISPR References and Information

    Type
    Collection
    ...analyze outcomes from cleaving nucleases (e.g. Cas9, Cas12a) and noncleaving nucleases (e.g. base editing...opens in a new window) A webtool that uses deep learning models for SpCas9 gRNA design and efficiency prediction...opens in a new window) A webtool that uses deep learning models for base editor gRNA design and efficiency...opens in a new window) A webtool that uses deep learning models for prime editor pegRNA design and efficiency...

  3. Rett Syndrome

    Type
    Collection
    ...protein function results in Rett syndrome is not clear, however, the ability to bind to methylated DNA ...ranscriptional R epressor D omain (TRD) the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator of Retinoic...receptor (NCoR/SMRT) interacting domain (NID) a nuclear localization signal (not essential for localization...RSRT cell line collection (186.8 KB) . To order, please email [email protected] The following cell lines are...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page...precise editing of mutations in the MECP2 gene. Please refer to Addgene's CRISPR Guide for a general introduction...your plasmids! To get started with your deposit, please click here ....

  4. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...are also available Non-toxic to living cells To learn more about this system, visit the New England Biolabs...website. For a comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag...permitting selective simultaneous labeling in a sample. Please see the note below for information on substrates...pSNAPf-H2B Control Plasmid SNAP-tag Control for nuclear localization 101123 pSNAPf-ADRβ2 Control Plasmid...pCLIPf-H2B Control Plasmid CLIP-tag Control for nuclear localization 101130 pCLIPf-Cox8A Control Plasmid...ACP-MCP-Tag plasmids - Substrate source (PDF, 46 KB) Please note: New England Biolabs (NEB) has discontinued...

  5. Optogenetics AAV Preps

    Type
    Collection
    ... 154948 CaMKII-somBiPOLES-mCerulean CamKII BiPOLES (soma-targeted) mCerulean Constitutive 5, 9, rg* Simon...154951 hSyn-DIO-somBiPOLES-mCerulean Syn BiPOLES (soma-targeted) mCerulean Cre dependent 5, 9, rg* Simon...Constitutive Serotype AAV1 AAV2 AAV5 AAV8 AAV9 AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  6. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...conformational change in the sensing region of the construct leads to a change in signal of a circularly permuted fluorescent...sensor (MARIO) A Transient Rise in Free Mg 2+ Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic ...Nagai Magnesium MagFRET-1 Mg 2+ sensor, based on Cerulean/Citrine MagFRET: The First Genetically Encoded...3;108(18):7351-6. Amy Palmer Zinc Cytosolic or Nuclear zinc-binding FRET biosensor (Zap) New alternately...through membrane lipid peroxidation, cytosolic release of CAVIN1, and downstream regulation of NRF2. bioRxiv...Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses. Proc Natl Acad...sensors for monitoring spatiotemporal serotonin release in vivo. bioRxiv 2023.05.27.542566. Yulong Li Serotonin...

  7. COVID-19 Resources

    Type
    Collection
    ... ordering from a for-profit entity, please see our Plasmid Distribution to Industry page. ...et al., 2020). FURIN plasmids - an enzyme that cleaves precursor proteins to a biologically active state... The SARS-CoV-2 S protein contains a potential cleavage site for furin proteases (Anderson et al., 2020...TACE plasmids - (ADAM17) a metalloprotease that cleaves hACE2, potentially increasing viral infectivity...Rottier, P. J. (2008). Cathepsin L functionally cleaves the severe acute respiratory syndrome coronavirus..., O., & Pöhlmann, S. (2014). TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2...

  8. Neurodegeneration Research Collection

    Type
    Collection
    ...Disease. These diseases persist for many years and lead to a major burden on the patients, their families.... Scientists are studying protein aggregation, learning about the blood brain barrier integrity, and using...spinal cord. Over time, a loss of motor neurons leads to limb weakness, difficulty speaking, swallowing...percentage (5–10%) are familial ALS cases having at least one other affected family member. These familial... individuals develop late-onset AD. There is no clear association between a gene and late-onset AD. It...libraries . Use the ultracompact and programmable endonuclease, TnpBmax , for genome editing. (Marquart et ...

  9. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...downstream of the sequence to be cleaved. This non-specific cleavage is thought to activate programmed...DNA. In bacteria, once they have recognized and cleaved the target RNA sequence, they adopt an enzymatically...enzymatically active state and can bind and cleave additional RNAs regardless of homology to the crRNA. This... CRISPR plasmids designed for RNA targeting. To learn more about RNA targeting and other CRISPR topics...

  10. Mammalian RNAi Tools

    Type
    Collection
    ...Dicer, an RNase III enzyme, binds the shRNA and cleaves it to produce siRNA. The siRNA then binds the RNA-induced... siRNA then binds the target sequence and RISC cleaves and degrades the mRNA. RISC includes argonaute ...argonaute protein 2 (Ago2), which facilitates cleavage of the target. RNAi using siRNA follows a similar mechanism...Since it is pre-processed, it can bypass the Dicer cleavage step and proceed to binding of RISC. For more ...containing other RNAi components, like argonaute, please search our full site . ID Plasmid Vector Type PI...

  11. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...NUSA tag and TEV cleavage pExp-NusA - Expression of protein in E. coli as TEV cleavable N-terminal His ...of target gene with N-terminal GST tag and TEV cleavage Transient expression - Gradia Lab plasmids for...lab pFB-LIC-Bse - Insect expression vector from Gileadi lab Worm unc-54, variety of worm gene promoters...Guide and the Michael Davidson FP Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid...myc-nuc - pEntry vector that adds a C-terminal nuclear localization signal pGADCg - Gateway yeast expression...

  12. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Microtubules 87422 LMNB1-mEGFP AICSDP-10 mEGFP Lamin B1 Nuclear envelope 87423 TOMM20-mEGFP AICSDP-8 mEGFP Tom20...114403 LMNB1-mTagRFP-T AICSDP-29 mTagRFPT Lamin B1 Nuclear envelope 114404 AAVS1-mEGFP (PGK) AICSDP-36 mEGFP...NUP153-mEGFP AICSDP-50 mEGFP Nucleoporin Nup153 Nuclear pores 114410 FUS-mEGFP AICSDP-64 mEGFP RNA-binding...fibrillar center) 133964 SON-mEGFP AICSDP-82 mEGFP SON Nuclear speckles 159744 DMD-mEGFP AICSDP-55 mEGFP Dystrophin...PCNA-mEGFP AICSDP-116 mEGFP Proliferating cell nuclear antigen (PCNA) DNA replication foci IPSC cell lines...

  13. Viral Production

    Type
    Collection
    ...each viral lot. To learn which specific QC experiments were performed on your lot, please contact us . Full...medium (OptiPro SFM) containing lentivirus is first cleared by low-speed centrifugation and then by filtration...responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for...the sample from the DNA tube all the way to the cleared viral preparation pool. Proviral Identity Confirmation...

  14. Ras Pathway

    Type
    Collection
    ...NF1 Neurofibromin 1 NFE2L2 Nuclear factor, erythroid 2 like 2 NFKB1 Nuclear factor of kappa light polypeptide...guanine nucleotide releasing factor RASGRP RASGRP1 RASGRP2 RASGRP3 RASGRP4 RAS guanyl releasing protein RASSF...activating pathways for cell growth and potentially leading to cancer. Constitutively active Ras is the most...

  15. Plant Plasmids and Resources

    Type
    Collection
    ...expression, and fluorescent plasma membrane and nuclear markers. Golden Gate (GreenGate) Alexis Maizel ...most popular plasmids for gene editing in plants. Please refer to Addgene's Plant CRISPR Resources for a...for Plants Plasmids for gene editing using Cas nucleases and base editors in monocots and dicots. CRISPR...expression, and fluorescent plasma membrane and nuclear markers. CRISPR Alexis Maizel 1000000151 CRISPR-Cas9...Arabidopsis Transformation Save Time with Transient Plant Leaf Transformations Engineering the Plant Genome Using...

  16. Control AAV Preps

    Type
    Collection
    ...James M. Wilson 105557 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG CB7 mCerulean Constitutive 1, 9 James M. Wilson...VCre-dependent Constitutive (non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  17. KLF Research Plasmids

    Type
    Collection
    ...Browse Addgene's collection of KLF plasmids and learn more about the scientists who have created and shared... Krüppel-like factors (KLFs) are a family of at least 17 DNA binding transcriptional regulators with diverse...collaborative efforts within the KLF community have lead to the establishment of this shared resource on ...encourage you to contribute to this community resource. Learn about the benefits of depositing plasmids with Addgene...

  18. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...cloning grade DNA, please let us know using the Cloning Grade DNA Suggestion Form . Please note that this ... (± 5 ng/µl) Buffer 10 mM Tris-Cl, pH 8.5 Price Please log in to your account and check the individual...I find cloning resources or scientific support? Please see our Addgene Protocols for resources you may...

  19. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...Hochschild and colleagues ( Dove et al., Nature 1997 ) and later modified by Pabo and colleagues ( Joung et...this Kit These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite...

  20. Antibody Production

    Type
    Collection
    ...Lab on the antibody webpage. To learn more about these antibodies, please visit the Institute for Protein...mammalian cells. To learn which specific QC experiments were performed on your lot, please email [email protected]...

  21. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...reports have shown that engineered zinc finger nucleases (ZFNs) are effective tools for making targeted...the spacer sequence between the half-sites (see Nuclease Expression Vectors for more details). IMPORTANT...open-source' engineering of customized zinc-finger nucleases for highly efficient gene modification. Maeder...mutagenesis in zebrafish using customized zinc-finger nucleases. Foley JE et al. Nat Protoc. . 2009. Dec 3. 4(...

  22. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ..., additional nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can...or Cas9 domains PE7 — addition of RNA-binding exonuclease protection factor La to PEmax to enhance pegRNA...tables below for CRISPR prime editing plamisds. To learn more about prime editing and other CRISPR topics...

  23. AAV Molecular Tools

    Type
    Collection
    ...-pre-mGRASP-mCerulean CAG-driven, constitutive Expression of pre-synaptic mGRASP-mCerulean for synaptic...Genome Engineering Tools These AAV encode Cas9 nuclease used in the CRISPR-Cas9 system for targeted genome...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  24. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pCS2plus-mCerulean 156-239-GGGS-TDP-43 TARDBP mCerulean CMV ALS Marco Morsch 162618 pCS2plus-mCerulean 156-...Parkinson's, FTD Michael Davidson 55431 mCerulean3-MAPTau-C-10 MAPT mCerulean3 CMV Parkinson's, FTD Michael Davidson...Sidhu 110061 pDUET-1-alpha-synuclein-mCerulean3-His6 SNCA His, mCerulean3 T7 Parkinson's Dan Mulvihill 110486...Lippincott-Schwartz 164214 PLEX-PGK-ANXA11-mCerulean ANXA11 mCerulean PGK ALS Jennifer Lippincott-Schwartz ...mRuby CMV ALS Jennifer Lee 216227 TDP-43 mCerulean TARDBP mCerulean CMV ALS Jennifer Lee 216709 pCAG-TSF-...If you do not see a particular gene of interest, please search our full plasmid collection using the search...Bacterial Yeast Other Promoter CMV T7 polH GAL Other Clear Filters ID Plasmid Name Gene Tags Promoter Disease...

  25. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Deisseroth AV-1-PV3248 105557-AAV1 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG Control James M. Wilson AV-1-PV3508 98928...Wickersham AV-9-PV3248 105557-AAV9 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG Control James M. Wilson AV-1-PV1090 105537...Plasmids are available for all the items listed below. Please let us know (Link opens in a new window) what you...you would like to see available in viral format. Please note this does not guarantee viral service, but...pENN.AAV.CB7.CI.TurboRFP.WPRE.RBG James M. Wilson Please contact [email protected] with any questions. Return...

  26. TALEN Plasmids and Kits

    Type
    Collection
    ...pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding specificity ...DNA-binding domain of a TALE, along with strong nuclear localisation tags and repress transcription initiation... BioSciences (N153AA, C63AA) and showed robust cleavage activity in several later studies. pCAG-T7-TALEN...

  27. Luciferase Plasmid Collection

    Type
    Collection
    ...Luciferase catalyzes the oxidation of a substrate, leading to the emission of photons. Its robust signal, ...sensitivity, wide dynamic range, and resistance to photobleaching have made luciferase a popular tool for scientists...making it ideal for a variety of applications. To learn more and plan your experiment, read our Luciferase...Artificial luciferases desined de novo using deep-learning approaches. Split Luciferase Complementation assays...

  28. Caltech Systemic Capsids

    Type
    Collection
    ...mice use LY6A to cross the blood-brain barrier, leading to strain-specific tropism (See S3 table (Huang...review performance data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER...using the Caltech serotypes in future publications, please acknowledge Viviana Gradinaru and cite as follows...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  29. Fluorescent Protein Guide

    Type
    Collection
    ...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ...fluorescent protein plasmids. Use this guide to learn more about the many applications of fluorescent ... gene expression, signaling pathways, and more. Learn More and Explore Addgene Blog: Fluorescent Proteins...

  30. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles.... Figure 1: Overview of NHEJ and HDR following cleavage by Cas9. Browse, sort, or search the tables below...CRISPR plasmids designed to introduce a DSB. To learn more about NHEJ, HDR, and other CRISPR topics, read...
Showing: 31 - 60 of 1015 results