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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 801 results for: MAK

Showing: 41 - 80 of 801 results

  1. NETRF

    Type
    Collection
    ...NETRF strongly encourages its funded scientists to make their published materials and resources available...

  2. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...Protocols gRNA Design Tools CRISPR Blog Posts To make targeted epigenetic modifications, researchers have...

  3. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ... require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system. The T375G mutation...

  4. Chemogenetics Plasmids

    Type
    Collection
    ...luciferase and a light-sensing optogenetic element, making a bimodal opto-chemogenetic actuator. When the ...

  5. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2 ...

  6. AAV Molecular Tools

    Type
    Collection
    ... vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our ...

  7. Mammalian RNAi Tools

    Type
    Collection
    ...Toolbox Optimized shRNA design in pSico with pSicoligomaker (Andrea Ventura lab) References Carthew, R....

  8. Recombinases AAV Preps

    Type
    Collection
    ... vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our ...

  10. AAV for Neuronal Tracing

    Type
    Collection
    ... vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our ...

  11. Chemogenetics AAV Preps

    Type
    Collection
    ... vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our ...

  12. Antibody Plasmid Collection

    Type
    Collection
    ...102. Joanna Bereta Sybody Generation Toolbox for making synthetic nanobodies (sybodies) against membrane...

  13. Microbiology Resources

    Type
    Collection
    ...Engineering blog post to see how Addgene depositors are making this possible in a range of organisms. Find CRISPR...

  14. Worm Expression Resources

    Type
    Collection
    ...hermaphrodite consists of 959 somatic cells, which makes them attractive for imaging and fluorescence microscopy...

  15. Zebrafish Plasmid Collection

    Type
    Collection
    ...nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart research model. With more...

  16. Control AAV Preps

    Type
    Collection
    ... vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our ...

  18. Lentivirus Plasmids

    Type
    Collection
    ...components Trono 63586 psPAX2-D64V 2nd Packaging For making Integrase deficient lentivirus Rawlings , Scharenberg...

  20. COVID-19 Resources

    Type
    Collection
    ...and Yeast expression Spike protein constructs for making VSV pseudo virus Mammalian constructs Due to the...

  21. Optogenetics AAV Preps

    Type
    Collection
    ... vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our ...

  22. Retrograde AAV viral preps

    Type
    Collection
    ... vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our ...

  23. Caltech Systemic Capsids

    Type
    Collection
    ... vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our ...

  24. Tetracycline Inducible Expression

    Type
    Collection
    ...conditional auxin-inducible degron system Masato Kanemaki 92099 AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep Tet-inducible...

  25. Biosensor AAV Preps

    Type
    Collection
    ... vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our ...

  27. CRISPR Guide

    Type
    Guide
    ...generating gRNAs makes CRISPR one of the most scalable genome editing technologies, making CRISPR perfect... Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. ...very close proximity to the region to be edited, making the PAM sequence (NGG) limiting. This PAM is abundant...Anti-CRISPR Small Precision Edits CRISPR can be used to make small, very precise edits of a handful of bases,...RNA-binding protein to stabilize pegRNAs Twin PE - two PEs make complementary edits to opposite strands Prime editing...the ability to target almost any genomic locus, makes CRISPR an ideal genome engineering system for large-scale...large-scale, forward genetic screening. CRISPR can make highly specific, permanent genetic modifications...

  28. Pouring LB Agar Plates

    Type
    Protocol
    ...pouring, you should stop pouring and re-make the gel-mix. If you’re making plates without any antibiotic you...resistance gene. The following protocol will allow you to make your own LB/agar plates with your antibiotic of ...-agar powder per L of molten agar you’d like to make. The precise mass you measure out will be based ... like to pour. For example: Because we’d like to make 20 plates, and our plates can hold a maximum of ...200 mL of media total. Importantly, we’ll actually make a bit more than 200 mL (~220 mL) just in case we...small errors in measurement. You should also always make a bit more gel-ager mix than you think you’ll need...an appropriately sized bottle for autoclaving. We make 400 mL of agar in 1 L bottles and 200 mL of agar...

  29. Molecular Biology Reference

    Type
    Guide
    ...these 4 nucleotides makes up the genetic code and provides the instructions to make every protein within...these bases makes up the genetic code and provides all the information needed for cells to make proteins ...Once you have constructed a plasmid, you can easily make an endless number of copies of the plasmid using... can be dissolved in dH 2 0. Addgene recommends making 1000X stock solutions and storing aliquots at -...into your LB medium at 1:1,000. For example, to make 100 mL of LB/ampicillin growth media, add 100 μL...of the known primer region with very few errors making it an efficient and reliable sequencing method....A,C,T, or G) is labelled with a different color making it easy to identify the order of the DNA strand...

  30. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ... such as pipetting error and sample evaporation. Make the master mix after all the samples have been added...Pro-Tip Once a validated standard curve is obtained, make a small aliquot of each standard (enough for 1 or...value of the standard starts to drift, it’s time to make a new one. When developing the assay multiple plasmids...do NOT treat your plasmid standard with DNase ** Make 6 serial dilutions, in duplicate, of your standard...quality of the sample dilution series is critical. Make sure to pipet each dilution up and down at least...standard curve and the sample dilutions. Pro-Tip Make sure that the qPCR is valid by checking to the following...you should observe differences in Ct values that make sense for your dilutions (~3.3 difference Ct for...

  31. Weighing Reagents Protocol

    Type
    Protocol
    ...you’ll need to make buffers, media, or other solutions. A key part of this task is making sure you’re weighing...weighing by looking for a weight range on the scale. Make sure that the weight of the material you’re weighing...add the material directly to the tube to weigh it. Make sure that your scoopula or spatula is clean, and...of your reagent, transfer it into your container. Make sure that any spills produced during the protocol...you have your reagents weighed out, you can begin making your solutions!...

  32. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ... growth. This conditioned medium will be used to make a cell solution later in this protocol. Isolating...solution into the conditioned medium prepared above to make a new cell solution at a concentration of 5 cells...concentration: 4 × 10 5 cells/mL To seed one 96-well plate, make 10 mL of a 5 cell/mL solution. Calculate the total...0.125 µL Because this is such a small volume, first make 1 mL of a 1:100 dilution of the homogenized cell...transfer 100 times that volume, which is 12.5 µL. To make the final 5 cell/mL solution, transfer 12.5 µL of... the same focal plane. Scan the entire plate and make note of each well in which you see growth. At this...

  33. Lentiviral Vector Guide

    Type
    Guide
    ...engineered lentiviral vectors have many aspects that make them useful research tools in a variety of cell ...plasmids also contain a deletion in the 3’ LTR to make them self-inactivating (SIN). This deletion is transferred... long-term, constitutive expression. In order to make stable cell lines, VSV-G is often put under an inducible...Stable Cell Lines Lentiviral vectors can be used to make stable cell lines due to their integration into ... by daughter cells. Inheritance of the barcodes makes lentiviral libraries popular for lineage tracing...

  34. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...number will continue to increase. This diversity makes rAAV vectors a very useful system for preferentially...capacity for long-term gene expression. These features make them ideal for gene therapy, neuroscience research...channelrhodopsins, halorhodopsins, and other optogenetic genes makes them suitable to be packaged in the relatively small...choice for the delivery of CRISPR/Cas9 elements, making it one of the most common methods for in vivo CRISPR-based...shown higher efficiency in delivering large genes makes use of split inteins, proteins that carry out a ...

  35. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...volume to make gels of other agarose concentrations (e.g., 2 g of agarose in 100 mL of TAE will make a 2% ...is very little difference between the two. Note: Make sure to use the same buffer as the one in the gel... Rule For each sample you want to load on a gel, make 10% more volume than needed because several microliters...For example, if you want to load 1.0 μg in 10 μL, make 1.1 μg in 11 μL. Reference Page Top Index...

  36. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...stocks of their plasmids. This way, when you want to make more plasmid DNA, the plasmid will already be in...screw top tube or cryovial and gently mix. Notes: Make the 50% glycerol solution by diluting 100% glycerol...liquid bacterial culture, take 500 μL of culture to make your glycerol stock before you begin your plasmid... shake the glycerol before freezing (5-6 times). Make sure that you see one uniform solution, and there...

  37. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...chew gum, or apply makeup in the laboratory. Before you start your experiment, make sure your workspace...and the appropriate contact times for each agent. Make sure you have enough space to work at your lab bench...extinguisher are also required to be present in the room. Make sure that you know where these are located before...

  38. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...38.5nm, add 385µl of water. After making your 100uM stock, immediately make a working concentration of each...their primers in liquid, normally sterile dH 2 O. To make a 100uM stock of any primer, add a number of µl ...each primer (10uM) by making a 1:10 dilution of the stock. For example, add 100µl of primer stock to 900µl...

  39. Kit Free RNA Extraction

    Type
    Protocol
    ...precipitation step, Option B) Glycogen (Optional) Caution Make sure to read the SDS (Safety Data Sheet) for safety...Solution D Protocol Before starting this protocol, make sure to prepare solution D (see reagent section ...freeze-thaw cycles of your entire RNA sample, consider making smaller aliquots of it and storing those in -80...

  40. Guide to Using Pooled Libraries

    Type
    Guide
    ...plasmid libraries, the plasmids must first be used to make virus. This pooled virus is subsequently used to...that an infected cell receives only one plasmid. To make sure that every plasmid is adequately represented...electroporation and maxiprep). If delivering as virus, make virus; this creates a pooled lentiviral CRISPR library...
Showing: 41 - 80 of 801 results