...sample. Ethanol carry-over from purification protocols is also a common concern, and while ethanol can reduce...residual guanidine thiocyanate.
Phenol is a unique case, as pure phenol absorbs strongly at ~270 nm and...experiments. The usual suspects, such as proteins, phenol, or guanidine salts, exhibit their own characteristic...ones most likely to impact absorbance readings are phenol, guanidine salts, and detergents (Figure 1C-D; ...means that even samples with quite high levels of phenol (up to 600 ppm according to Loughrey and Matlock...yield “good” A260/280 and A260/230 ratios. Instead, phenol is more likely to shift the peak of your sample's...towards 270 nm. If your purification protocol uses phenol, then it is important to confirm that your sample...
