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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,051 results for: FER

Showing: 141 - 160 of 1051 results

  2. CRISPR Plasmids and Resources

    Type
    Collection
    ... activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor peptide, ...

  3. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

  4. Synthetic Biology - Overview

    Type
    Collection
    ...Negative Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard...

  5. AAV Molecular Tools

    Type
    Collection
    ...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

  7. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

  8. Brain Armamentarium

    Type
    Collection
    ...molecular genetic reagents to gain access to many different brain cell types. The expanded collection of Armamentarium...

  9. The Pleiades Promoter Project

    Type
    Collection
    ...NLS N/A CAG promoter pEMS1488 intron-lacZ/NLS References Portales-Casamar, E., Swanson, D. J., Liu, L....

  11. Genetic Code Expansion

    Type
    Collection
    ... directed evolution to charge the tRNA with a different amino acid. They are typically expressed from ...

  12. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Plasmids scFv Plasmids Nanobody Plasmids Protocols References The UC Davis/NIH NeuroMab Facility (Link opens...NeuroMab Protocols (Link opens in a new window) References Andrews NP, et al. 2019. A toolbox of IgG subclass-switched...

  13. Molecular Biology Reference

    Type
    Guide
    ...Educational Resources Molecular Biology Reference Molecular Biology Reference Jump To: Molecular Biology Basics...contain a reporter gene (for example, luciferase or GFP) that offers a read-out of the activity of the genetic... C EDQVDPRLIDGK S Tag KETAAAKFERQHMDS SB1 PRPSNKRLQQ Webpage and Blog References Addgene's blog , including...Addgene's molecular biology reference guide, including information on molecular genetics, plasmids, sequencing...verification Resources Genetic Code Webpage and Blog References Protocols Origins of Molecular Genetics The concept... Joshua Lederberg coined the term plasmid, in reference to any extrachromosomal heritable determinant....bacterium to another by a type of horizontal gene transfer (conjugation), usually providing a benefit to ...

  14. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...optimizing your transfer buffer.  Adjusting the buffer For wet and semi-dry transfers, you can adjust ...methanol concentration in transfer buffer is < 20% Wet transfer Increase transfer time Increase voltage...decrease alcohol in transfer buffer Small proteins: Over-transferring Transfer with two membranes...increase alcohol in transfer buffer Mixed proteins: Transferring issues Transfer with two membranes...be under-transferring.  Membrane pore size Membranes come in different pore sizes for different proteins...large.  Transfer method   Wet transfers are more efficient, so if you’re struggling to transfer a large...consider using a wet transfer with a long transfer time. Be aware that the transfer works by electricity...

  15. Technical Design of a Western Blot

    Type
    Blog Post
    ...There are many different lysis buffers available commercially, and most common buffers have recipes available...Types of transfers Your transfer options are wet, semi-dry, or dry/electroblot. A wet transfer is the most...  Note that each type of transfer requires different equipment. Wet transfers can be done in an insert... while semi-dry transfers require a semi-dry transfer cell. Dry/electroblot transfers only work in the...is typically sufficient.  Lysis buffer When choosing your lysis buffer, consider the subcellular location...and Tris gels run proteins in different patterns, due to their differing chemistries. You therefore cannot... 12% 10–250 kDa Different sizes 4–12% gradient 4–250 kDa Different sizes 4–20% gradient...

  16. Lentiviral Vector Guide

    Type
    Guide
    ...packaging genes. However, they are different viruses and require different isoforms of these packaging components...second-generation transfer plasmids as well. Third-generation plasmids include (Figure 4): Transfer plasmid — ...vectors separate transfer, envelope, and packaging components of the virus between different plasmids. The...activate the promoter on the transfer plasmid. Many of the lentiviral transfer plasmids that have been deposited...genes. From an experimental standpoint, the main difference between lentiviruses and gamma-retroviruses is...three (or four, for third generation) plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA ...systems, due to a wide range of infectivity for different cell types (known as tropism). Lentiviral vectors...

  17. Deep Dive: qPCR

    Type
    Blog Post
    ...melting profiles (Dwight, 2011).  Reference Genes  Choosing a set of reference genes deserves more attention...attention than it usually receives. Different reference genes have shown to have as much as sixfold variation...Passive Reference Dyes Whatever your pipetting method, some variation will occur. A passive reference dye,...Liu and Saint No Raw Data Reference and target genes can have different efficacies DART-PCR No ...will soon become a qPCR master. References and Resources  References Biassoni R; Raso A. (eds.), Quantitative...mRNA, which can be used as a proxy measure for differences in protein expression. (If you're just getting...assume that qPCR can accurately detect expression differences of twofold or greater with good statistical significance...

  18. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ... wet transfer system the sandwich is completely submerged in a tank filled with a transfer buffer formulated...systems efficiently transfer a range of protein sizes in minutes without any transfer buffer. These systems...electric current. The wet transfer process is very effective in transferring a broad range of protein ...overnight.  In contrast, a semi-dry transfer only requires enough buffer to saturate the sandwich and typically...validated for immunoblotting. Different immunoassays detect proteins in different states. For example, in immunoprecipitation... other antibody topics. Resources and references References Aydin S (2015) A short history, principles...western, proteins are: (1) separated by size, (2) transferred to a membrane, and  (3) detected using antibodies...

  19. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...heterogeneous population, cells behaving differently (e.g. different growth properties, migration abilities... modified cells are transferred into an animal and the behavior of the transferred cells is followed. ...targeting all differentially expressed genes that are known drug targets, or all differentially expressed ...best gRNAs by the depositing lab. The different labs use different algorithms to calculate which would ...were not found in the reference library. It’s not uncommon that genes have different names, and the software...generation sequencing to identify which gRNAs are differentially expressed in the separate groups and, as a ...is not targeted by only one gRNA, instead many different gRNAs are used for each gene. At the moment, 13...

  20. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...Addgene blog Plasmid Preps: Different Purity, Different Quantities, Different Uses Plasmids 101: Optimizing...lysis buffer ratio and result in incomplete lysis. You may be able to scale the amount of buffers used ...TakaraBio) offer yeast-plasmid specific kits.  Medium/High-throughput – Many manufacturers offer bead- and...quantity.   Figure 1: Plasmid preps fall into different categories based on their relative qualities and...fairly self explanatory and achieved by performing different sized preps, such as miniprep, midiprep, etc. ...what do we mean by DNA quality? There are a few different issues that can arise during the plasmid purification...which can happen if you leave the sample in a lysis buffer for too long or if you get a little overzealous...
Showing: 141 - 160 of 1051 results