...pluripotency during embryonic development. This “reprogramming” of the cell to an embryonic state holds immense...cell lineages. Pluripotent stem cells, such as embryonic cells, can potentially give rise to any other ...limited to related cell lineages. Pluripotent embryonic and multipotent adult stem cells have been studied...removes the possible ethical concerns related to embryonic stem cell use and has the major advantage that...Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors...Mouse Direct Reprogramming of Fibroblasts into Embryonic Sertoli-like Cells by Defined Factors. Cell Stem...via microRNA-dependent and -independent pathways. EMBO Mol Med. 2017 Aug;9(8):1117-1131. Parmar Fibroblasts...

...Transfer proteins from the gel to a membrane. Incubate the membrane with an unconjugated primary antibody...block the membrane to remove excess antibody and prevent unwanted binding. Incubate the membrane with a ...Wash and block the membrane. Activate the HRP with a substrate kit. Image the membrane using either X-ray...tears, saliva, mucus) where it protects mucosal membranes Dimer with a valency of 4 IgA1; IgA2 IgD Coexpressed...short peptide linkers. Diabodies can be used for assembling protein nanostructures. Figure 2: An antibody...recognizes your primary antibody source species. Remember that recombinant antibodies will also have a source...

...regulators of membrane traffic, they also play critical roles at cilia, centrosomes, the plasma membrane, lipid... collection of ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs. Plasmid...regulatory GTPases have long been characterized as members of the RAS superfamily, though recently have been...signaling activity. Locations of ARF Superfamily members. Figure from Szutl, et al 2019 PMID: 31084567 .... . More information about ARF Superfamily members can be found in: ARF GTPases and their GEFs and GAPs...

...e.g. maize and Arabidopsis) and vectors for easy assembly of one or more gRNA expression cassettes - Qi-Jun...intronized Cas9 series of vectors and DNA modules for assembly of CRISPR/Cas9 constructs - Sylvestre Marillonnet...fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants...creating plant expression vectors: A modular plasmid assembly kit for multigene expression, gene silencing, ...Parts II and Infrastructure Kit for hierarchical assembly of plant multigene constructs - Johannes Stuttmann...cloning chassis and set of functional parts for assembling plant transformation vectors - Daniel Voytas....

...expression vectors for TAL effector repeat arrays assembled using the REAL, REAL-Fast or FLASH methods...fragments encoding TAL effector repeat arrays (assembled using the REAL, REAL-Fast or FLASH methods) can...Additional details about the REAL, REAL-Fast, and FLASH assembly methods can be found on the TALengineering website...amino acid sequences (i.e. only the details of the assembly are different among the three methods, the final...REAL TALE Repeat Plasmids in the Joung Lab REAL Assembly TALEN Kit . Vectors encoding wild-type FokI nuclease...

...Adenoviral A block for the AdenoBuilder genome assembly system. Block 6 with deletion in E3 and insertion...Adenoviral A block for the AdenoBuilder genome assembly system; consolidates the regions present in pAd5...Adenoviral A block for the AdenoBuilder genome assembly system. Block 1 with deletion in E1 and insertion...adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction. ID Kit Description PI ... Plasmids contain modular inserts for seamless assembly of multiple mutations, alterations, or transgene...

...deposited tools and resources, or read our guide on assembly standards. Browse Plasmids by Microbe System Plasmids...chromosomes - Endy and Shearwin Labs Ralstonia-GG-Kit : Assembly of Ralstonia solanacearum species complex (Rssc...lipolytica Golden Gate tool kit : Integrative vector assembly of up to three transcription units in the oleaginous...circuit design in E. coli - Freemont Lab MoClo-YTK : Assembly of single and multi-gene constructs for S. cerevisiae...perform your synbio experiment. Synthetic Biology Assembly Standards Guide Bacterial Expression Systems :...

...Methods, September 2007, Vol. 4 No. 9, pp. 741-6 Shemiakina et al. : Nature Communications, November 2012,...Addgene has assembled a collection of empty plasmid backbones with different fluorescent tags for you...result in a diverse set of colors. Addgene has assembled a collection of empty plasmid backbones with different...Stepanenko et al. : BioTechniques, Vol. 51, No. 5, November 2011, pp. 313–327 Scholarpedia : Entry by Robert...Shaner et al. : Journal of Cell Science, Vol. 120, December 2007, pp. 4247-4260 Shaner et al. : Nature Methods...

...method Modular Assembly Engineering Reagents - Engineer zinc finger arrays via Modular Assembly method Nuclease...finger proteins deposited by Zinc Finger Consortium members like the Joung Lab, Voytas Lab, and Wolfe Lab Plasmid... engineered zinc finger technology. Consortium members Keith Joung and Daniel Voytas have deposited at...

...community. MCC members gain access to mentors, virtual and local professional development, membership to a local...research opportunities to their students and other members of the MCC community. They support educators interested... CUREs, and opportunities to mentor future MCC members. Find a regional hub (Link opens in a new window...

...for Targeted Neuronal Manipulation Meeting Date September 21, 2018 Contact Us Questions? Contact us at AAVED...scientific outcomes. Event Details Date: Friday September 21, 2018, 9 AM - 5 PM Location: Addgene, 75 Sidney...workshops. Tentative Meeting Agenda Thursday, September 20 6:00 PM-8:00 PM Group Dinner Relax and Connect...Connect with Fellow Speakers and Attendees Friday, September 21 9:00 AM Breakfast and Welcome Melina Fan 9:...

...DR5 Tumor necrosis factor receptor superfamily, member 10b E2F-1 E2F transcription factor 1 Fas Fas cell...protein 3 KAI CD82 molecule Maspin Serpin family B member 5 MDM2 MDM2 proto-oncogene, E3 ubiquitin protein...nexin, plasminogen activator inhibitor type 1), member 1 PERP TP53 apoptosis effector PIDD p53-induced...G2 arrest mediator candidate Scotin Shisa family member 5 Sestrins SESN1 SESN2 SESN3 Sestrins 1, 2, or ...

...developmental biology. With its rapid development (embryogenesis is complete in ~3 days), short generation time...ease of care and breeding, and transparency of the embryo and larvae, it is easy to culture large populations...Project - Access the latest reference sequence assembly for the zebrafish genome. Zebrafish Model Organism...information about when genes are active in developing embryos. zfRegeneration - A database for gene expression...

...Resources Background Ras is a small GTPase and is a member of the G protein (guanine nucleotide binding protein...Ras signaling cascade that starts at the plasma membrane. Cancer Impact Ras is ubiquitously expressed in...RASSF8 RASSF9 RASSF10 Ras association domain family member RB1 Retinoblastoma 1 RCE1 Ras converting CAAX endopeptidase... in brain RHO RHOA RHOB RHOC Ras homolog family member ROCK ROCK1 ROCK2 Rho associated coiled-coil containing...

...for REAL (Restriction Enzyme And Ligation) TALEN assembly containing single TALE repeat domains.... as the REAL (Restriction Enzyme And Ligation) assembly method ( Sander et al., Nat Biotechnol. 2011 )...individually or as a set as part of the Joung Lab REAL Assembly TALEN Kit , which also includes the four TALEN...

...plasmids encode for microbial opsins, which are membrane channels and pores derived from algae and archaebacteria...ChR2) from Chlamydomonas reinhardtii . ChR2 is a membrane channel that opens in response to blue light and...properties as well as improve the expression and membrane targeting in host cells. Other microbial opsins...

...human genome with approximately 450 identified members. Many SLCs have mutations associated with disease...RESOLUTE is a public-private partnership of 13 members from industry and academia financed by the Innovative...generate antibody/ high-affinity ligands. Data is embedded in an integrated knowledgebase that features, ...

...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering SynBio...repressors, activators, and more. Also contains pre-assembled genetic circuits such as logic gates and higher...System (Plant) MoClo Modular Cloning Modular PUF Assembly Kit Optogenetics Pertz Lab cpFRET Kit Rinehart...

...plasmid-based antibodies. Description Article PI One-step assembly of antibody heavy- and light-chain DNAs into a...making synthetic nanobodies (sybodies) against membrane protein targets. Synthetic single domain antibodies...antibodies for the conformational trapping of membrane proteins. Elife. 2018 May 24;7. and Generation of...

...TGCCTGGCACAGGGGCTctccaccatgctggccAACCTGTTCTCAATGA kinesin-family-member-13a TAL3106 & TAL3107 TGTTCTCTGTTTGAGCGAgtctccacacagcagaGCGACAGTAACAGCTTCA...TCTTCTTGTGCTCTTATTggcaggtacctggtgacTCACCTTATGGGCGCTGA matrixmetallopeptidase15 (membrane-inserted) TAL3484 & TAL3485 TGATCTGTTCCTGGTAGCcgttcatgagctgggcCATGCCCTTGGACTGGAA...TGATCTGTTCCTGGTAGCcgttcatgagctgggcCATGCCCTTGGACTGGAA matrixmetalloproteinase14a (membrane-inserted) TAL3486 & TAL3487 TGTTCCTGGTGGCGGTGCatgaattgggtcatgcaCTTGGTTTGGAGCACTCA...