CRISPR Plasmids - Empty gRNA Vectors
Type
Collection
... for Cloning 67720 Worm NotI/BamHI none S. pyogenes Colaiacovo pRB17 52527 Fly blunt end cloning none ...yes, cut N. meningitidis Thomson pSmart-Nm-sgRNA-BbsI 49157 Mammalian U6 none N. meningitidis Thomson pXCas9H840A...none N. meningitidis Pederson pLH-stsgRNA1.1 64116 Mammalian/Lentiviral U6 none N. meningitidis Pederson...none N. meningitidis Pederson pLH-stsgRNA3.1 64118 Mammalian/Lentiviral U6 none N. meningitidis Pederson...the same cell at the same time. Single plasmids containing both the gRNA and a Cas protein act as an all-in-one...or nick. Selection , such as Puromycin or EGFP Cloning enzyme used for insertion of your gRNA sequence...used with, such as S. pyogenes, S. aureus, N. meningitidis, etc. gRNA scaffolds are specific for the species...