Bacterial Expression Systems
Type
Collection
...testing transcription factor binding to reporter plasmid pH3U3-zif268 (omega) . Transcription factors ... answer many interesting questions. Does my transcription factor bind to a particular sequence? Do these...wild-type Cas9 from S. pyogenes for inducing double stranded breaks. For a more comprehensive list of our bacterial...see here . pGRG Series Various Plasmids Tn7 Transposition Nancy Craig A gene of interest cloned into one... one of these plasmids can be transposed into the attTn7 attachment site found in many enterobacterial...enterobacterial genomes. The bacteria of interest are transformed with the appropriate construct, grown at the permissive...permissive temperature (32°C) to allow transposition, and the construct is then cured by growing at the ...