...spike pseudotyped virus. It also lists several luciferase and fluorescent reporter plasmids that have been...information on these genes, see the links and references below: ACE2 - (Human Angiotensin Converting Enzyme...Material. Rauch J, et al. bioRxiv 2020.04.20.052159 . Jennifer Doudna's lab has utilized Cas12a’s non-specific...Addgene's Protocol for RNA Extraction Without A Kit References ACE2 - Andersen, KG, et al, 2020, Nature Medicine...2014, J Virol. TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry...

... CRISPR Interfere CRISPR Plasmids: Interfere Browse CRISPR Plasmids By Function...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...like KRAB, can knock down gene expression by interfering with transcription. Design your gRNA to target...

...express nucleases in different cell types. Zinc finger arrays can be transferred in-frame to these vectors...plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays created using ZFC...either OPEN or modular assembly can be easily transferred in-frame to any of these vectors using unique...

... EGFP under control of human synapsin1 promotor. Ofer Yizhar 198513-AAV5 pAAV_hSyn-PdCO-EGFP-WPRE Expresses... EGFP under control of human synapsin1 promotor. Ofer Yizhar 198516-AAV1 pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE...Ef1a promoter after Cre-dependent recombination. Ofer Yizhar 198516-AAV5 pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE...Ef1a promoter after Cre-dependent recombination. Ofer Yizhar 202199-AAV1 pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE...synapsin1 promotor after Cre-dependent recombination Ofer Yizhar 202199-AAV5 pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE...synapsin1 promotor after Cre-dependent recombination Ofer Yizhar Antibodies Addgene distributes ready-to-use...

...opto-chemogenetic actuator. When the luciferase enzyme oxidizes its substrate (luciferin), it generates light that...luminescent opsins: fusion proteins of a light-emitting luciferase and a light-sensing optogenetic element, making...or other terms. Looking for viral preps? We also offer ready-to-use AAV preparations of many chemogenetics...

...spectra) of different types of opsins and optical switches available in these tables, refer to Addgene'...dimerization, localization or activity of many different types of proteins. For a full description (including...or other terms. Looking for viral preps? We also offer ready-to-use AAV preparations of many optogenetics...

...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end ...Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a ...like KRAB, can knock down gene expression by interfering with transcription. Design your gRNA to target...

...on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end ...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...ID Plasmid Gene/Insert Promoter PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a ...like KRAB, can knock down gene expression by interfering with transcription. Design your gRNA to target...

...Zebrafish Tools in our Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...Ac/Ds transgenic system enabling CRISPR/dCas9-interference (CRISPRi) modulation of enhancers and non-coding...previously resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically controlled Cre/loxP system... Zebrafish Genome Project - Access the latest reference sequence assembly for the zebrafish genome. Zebrafish...

...on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end ...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a ...like KRAB, can knock down gene expression by interfering with transcription. Design your gRNA to target...

...performing phosphoproteomic experiments in need of reference spectra or phosphopeptide standards for quantitative...FACS). The mode #2 phosphosite library with four different phosphobinding domains (14-3-3β, 14-3-3σ, the ...phosphorylation positional isomers are allocated to different subpools, allowing precise knowledge of phosphorylated...Library 111708 Mode #2 Library (NEDD4-2 WW2 domain) References & Protocols Iterative Synthetically Phosphorylated...

...derivative of tetracycline, doxycycline (dox), is a preferred effector for tetracycline transregulation. Dox...single copy in mammalian cells; Expresses firefly luciferase hairpin and GFP under pTREtight promoter None...pSBtet-GP Sleeping Beauty transposon system; has luciferase in cloning site; see article for additional selection...neuronal-specific promoter and enhancer options tTA Off Uney References Tight control of gene expression in mammalian...

...Plasmid Collection INTRSECT Implementation Plasmids References The development of powerful molecular reagents...pAAV-Ef1a-Con/Fon/Von-GCaMP6M Cre AND Flp AND Vcre References Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee...Conzelmann KK, Staiger JF. 2019. Mapping Brain-Wide Afferent Inputs of Parvalbumin-Expressing GABAergic Neurons...Link opens in a new window) Poulin JF, Caronia G, Hofer C, Cui Q, Helm B, Ramakrishnan C, Chan CS, Dombeck...

...retrograde serotype from the Alla Karpova and David Schaffer laboratories. These viral vector preparations ...titering by SYBR green qPCR , confirmation of the transfer plasmid with PCR, purity assessment by silver ...viral project team in collaboration with David Schaffer (University of California, Berkeley). Don’t See...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...pooled CRISPR libraries in Addgene’s collection differ based on some important criteria, and it is important...page. Find Pooled Libraries of Interest Click on different properties to create a custom filtered list of... 67989 Knockout Human Yusa 3rd 5 90,709 Human Interferon-Stimulated Gene CRISPR Knockout Library 125753...

...diverse roles in processes such as cell proliferation, differentiation, inflammation, metabolism, fibrosis...pages with information on available plasmids, references, and links. KLF1 KLF2 KLF4 KLF5 KLF6 KLF Plasmids...

...resistance. Protocols & References A transcription factor atlas of directed differentiation. Joung J, Ma S, Tay...The Human TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled Library The MORF library...

...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...the simplicity of gRNA design, programmable for different genomic loci, capable of detecting multiple genomic...fluorescence in situ hybridization (FISH), CRISPR imaging offers a unique method for detecting the chromatin dynamics...

...Service Details Plasmids Available FAQ Addgene is offering Cloning Grade DNA (cgDNA) for over 200 plasmids...Volume 20 µl Concentration 100 ng/µl (± 5ng/µl) Buffer 10 mM Tris-Cl, pH 8.5 Price Please log in to your... methods of quantification may yield slightly different DNA concentrations. Can I directly transfect cloning...

... promoters or enhancers of genes of interest. Different flavors of activators were constructed, with VP48...Gateway donor vectors [48218~48221] and can be transferred to Gateway destination vectors (e.g., pmax-DEST...Lifetechnologies Gateway Destination Vectors ) to adapt to different systems by LR clonase reaction. These plasmids...