We narrowed to 75 results for: cat.1

...Bacterial TAG Farren Isaacs 73545 pEvol-pAcFRS.1.t1 pAcFRS.1.t1 E. coli p-acetyl-l-phenylalanine (pAcF) Bacterial...Bacterial TAG Farren Isaacs 73547 pEvol-pAzFRS.1.t1 pAzFRS.1.t1 E. coli p-azido-l-phenylalanine (pAzF) Bacterial...recognized by the cognate orthogonal tRNA. Figure 1: Use of synthetase and target plasmids in genome code...7FTrp) Bacterial TAG Thomas Huber 207639 pEVOL-NBK-1 PylRS M. mazei Pyrrolysine Bacterial Cole DeForest...a ncAA into a protein of interest, four key modifications to the standard translation machinery are required...with a different amino acid. To achieve these modifications, two plasmids are generally used. Once these...amino acids (ncAA) in a protein of interest. Applications By making small changes in selected amino acids...

...Insert Promoter PI Publication 64325 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO-1 Fujii Efficient isolation...Insert Promoter PI Publication Return to top Base Edit ID Plasmid Promoter PI Publication Return to top Cut...Promoter PI Publication Return to top Interfere ID Plasmid Gene/Insert Promoter PI Publication Return to...Promoter PI Publication Return to top Prime Edit ID Plasmid Gene/Insert Promoter PI Publication Return to...isolation of specific genomic regions and identification of associated proteins by engineered DNA-binding... to top RNA Targeting ID Plasmid Promoter PI Publication Return to top CRISPR Resources Addgene has a ...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

...the dCas9-activator to your specific locus. Figure 1: Overview of CRISPR activation. Created with BioRender.com...Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to ...Marker PI Publication Return to top C. elegans ID Plasmid Gene/Insert Promoter PI Publication Return to...Drosophila Plant C. elegans Yeast Resources Catalytically dead dCas9 fused to a transcriptional activator...Drosophila ID Plasmid Gene/Insert Promoter PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a ...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

...Expression mEGFP 488 507 34 6 Monomer (A206K) pmEGFP-1 - Mammalian Expression mEGFP-N1 - Mammalian Expression...TurboRFP-pBAD - Bacterial Expression tdTomato 554 581 95 4.7 1 h Tandem-dimer pCSCMV:tdTomato - Mammalian Expression...-pBAD - Bacterial Expression mRFP1 584 607 13 4.5 1 h Monomer pcDNA3-mRFP - Mammalian Expression pMXs-...Mammalian Gateway Destination Vector pENTR4-HaloTag (w876-1) - Gateway Entry Vector pCS2+/C-Halo , pCS2+/N-Halo...pSNAPf-C1 - Mammalian Expression pENTR4-SNAPf (w878-1) - Gateway Entry Vector pCS2+/C-SNAPf , pCS2+/N-SNAPf...opens in a new window) or plasmids' original publications. Note for all tables: Brightness is the product...backbones for general cloning, expression, or other applications? Visit our Empty Backbones page or search our...

... edits via homology-directed repair (HDR). Figure 1: Overview of Cas9 nickase cleavage and repair. Browse...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top Drosophila ID Plasmid Gene/Insert Promoter ...Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...Marker PI Publication Return to top Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker ...Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

...collateral RNA degradation seen in bacteria. Figure 1: Overview of RNA targeting. Browse, sort, or search...Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert PI Publication Return to top ...top Plant ID Plasmid Gene/Insert PI Publication Return to top CRISPR Resources Addgene has a large selection...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

...research and innovation programme and EFPIA. Figure 1: Representation of RESOLUTE mission. The combination...632-675. https://doi.org/10.1038/s44320-025-00109-1 (Link opens in a new window) PMID: 40355756 (Link ...Updated Content last reviewed on 30 January 2025. Catalog items are updated more frequently....

...Buenrostro JD, Regev A, Zhang F. Cell 2023 Jan 5; 186(1):209-229. PubMed (Link opens in a new window) . TF... has a unique 24 bp barcode that simplifies identification of the transcription factor isoform in pooled...used for screening transcription factors for applications such as cellular programming or cancer drug ...barcoded with a 24 bp sequence, to simplify identification during screening. Library ID PI Description...

...direct identification of molecules associated with a genomic region of interest in vivo. Figure 1: Overview...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR...conventional methods for genomic purification, CRISPR-based purification methods are more straightforward and...plasmids designed for the purification of genomic loci. To learn more about purification using CRISPR and other...chromatin immunoprecipitation (ChIP) to allow purification of any genomic sequence specified by a particular...DNA-binding molecule-mediated ChIP) system, catalytically inactive dCas9 is used to purify genomic DNA... can be fused to dCas9 or gRNA for efficient purification. Various epitope tags including 3xFLAG-tag, ...

...with target sites throughout the chromosome. Figure 1: Overview of visualizing genomic loci using CRISPR...Visualize Genomic Loci Mammalian Resources Using catalytically inactive Cas9 (dCas9) fused to a fluorescent...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a ...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

...occurs at a much lower efficiency than NHEJ. Figure 1: Overview of NHEJ and HDR following cleavage by Cas9...Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to ...introduce a double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...Promoter Selectable Marker PI Publication Return to top C. elegans ID Plasmid Gene/Insert Promoter Selectable...Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker ...Marker PI Publication Return to top Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...

...some mammalian systems have been developed. Figure 1: Overview of the basic CAST mechanism. Created with...Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Selectable Marker PI Publication Return...integration of large fragments of DNA at specific locations. The Cas effector lacks nuclease activity, instead... (comprised of multiple components) to target locations in the genome defined by a guide RNA. The transposase...insertions — meaning they may not be suitable for applications where orientation of the insert is important...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

...gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids 159654...pX458 plasmid family. They have made several modifications to the the pX458 plasmid to expand its usefulness...developmental studies. Key plasmid features and pX458 modifications: replacement of the Cbh promoter with EF1alpha...Plasmids from Collection ID Plasmid Description PI Publications Kaufman, M. L., Goodson, N. B., Park, K. U.,... alters photoreceptor and bipolar cell fate specification, yet differs from deleting both genes . Development...

...activation-induced cytidine deaminase (AID). Figure 1: Overview of CRISPR base editing. Browse, sort, or...editors are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase...and are thus well suited to directed evolution applications. Examples of these base editing systems include...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable...Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI...PI Publication Return to top Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...

...fluorescent proteins in budding yeast. Sci Rep, 9 (1):2234. https://doi.org/10.1038/s41598-019-38913-z ...TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression Plasmids for constitutive overexpression...marker. ID Plasmid Gene/Insert Selectable Marker Publication Additional Resources Addgene Kits MoClo-YTK Yeast...Updated Content last reviewed on 12 August 2025. Catalog items are updated more frequently....

...for brain circuit mapping and connectomics. Figure 1: Reconstruction of a sparse set of neurons across ...components can be used separately for additional applications. Park & Sheridan et al. (2025) use plasmids ...circuit mapping and reconstruction. Potential applications could also include using individual or subsets...

...affecting females at an incidence of approximately 1:10,000. It is characterized by apparently normal development... the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator of Retinoic acid and Thyroid hormone... disruptions, missense mutations located in the MBD, and truncations prior to the NID are more severe ...even within patients with the same mutation, the location and type of MECP2 mutation can be a strong predictor...Line Mutation (DNA) Background Strain Purpose Publication PI Mecp2 NLucTom Knock-in of NLuc-tdTomato at...DNA) Mutation (protein) Sex Source of Material Publication PI N126I A377T N126I M Fibroblasts & iPSC (Link...

...particular viral spike protein (MERS, CoV-1, CoV-2), modification, or variant. ID Plasmid Description Mutations...vesicular stomatitis virus G) are packaged as replication-defective virus particles with the SARS-CoV-2...protein is inefficient for pseudotyping; several modifications may enhance infectious particle production, ...

...and interrogate with conventional reagents. Figure 1: Axon guidance cues and receptors regulate the development...Antibody Description Isotype Reactivity Recommended Applications PI Browse Netrin, DCC, and IGDCC3 (PUNC) Antibodies...Antibody Description Isotype Reactivity Recommended Applications PI Browse ROBO and Slit Antibodies ID Recombinant...Antibody Description Isotype Reactivity Recommended Applications PI Return to top Synaptic Cleft IPI’s Synaptic...Antibody Description Isotype Reactivity Recommended Applications PI Return to top Integrins Integrins are a family...Antibody Description Isotype Reactivity Recommended Applications Return to top Basic Protein Tools IPI has developed...Antibody Collection page to browse Addgene’s full catalog of antibodies that target epitope tags and other...

... Caltech and can help guide your decision. Figure 1: Decision tree for choosing your AAV capsid. Your ...tdTomato Control Boyden 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Fishell 99130 pAAV-mDlx-NLS-mRuby2...Available PHP.eB AAV ID Name Promoter Description Category PI Controls 28306 pAAV-FLEX-tdTomato CAG tdTomato...Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-FLEX-tdTomato CAG tdTomato, Cre-...Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP CAG EYFP, Cre-dependent...Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden MaCPNS2...Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden CAP-B10...