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Showing: 61 - 80 of 135 results

  1. Validated gRNA Sequences

    Type
    Collection
    ...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The table below lists gRNA...the particular conditions of the experiment as described in the associated article (listed below by PubMed...pyogenes, S. aureus etc.) was this gRNA sequence designed for? A given gRNA sequence may only be compatible...within promoters. When possible, the categories described on Addgene's CRISPR Plasmids and Resources page... to indicate the Cas9 application the gRNA was designed to accomplish. Validated gRNA Sequence Datatable...

  2. Deisseroth INTRSECT Collection

    Type
    Collection
    ... experimental design. How-to guide (Link opens in a new window) for the molecular design and testing of...interesting in implementing intersectional experimental design. The Deisseroth Lab maintains a Standard Operating...Gray Facilitate the Itch-Scratching Cycle via Descending Regulation. Neuron 101(1):45-59. PubMed (Link...

  3. Individual REAL TALE Repeat Plasmids

    Type
    Collection
    ...recently described a rapid and robust protocol for constructing TALE repeat arrays of any desired sequence...Joung Lab REAL Assembly TALEN Kit , which also includes the four TALEN expression vectors. More detailed...

  4. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To make targeted epigenetic...catalytically dead dCas9 to epigenetic modifiers. Design your gRNA to target a specific promoter or enhancer...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

  5. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically dead dCas9...gene expression by interfering with transcription. Design your gRNA to target your gene of interest’s promoter...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

  6. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically dead dCas9... can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

  7. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR/Cas nickase mutants...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...Selectable Marker PI Publication Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication...

  8. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type VI CRISPR systems,...regardless of homology to the crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require...encyclopedia of CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

  9. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully functional CRISPR...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...or search the tables below for CRISPR plasmids designed to introduce a DSB. Plasmids are available for...

  10. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use in ...CRISPR plasmids that have been designed for use in zebrafish. CRISPR...

  11. Optogenetics Plasmid Collection

    Type
    Collection
    ...many different types of proteins. For a full description (including peak response spectra) of different...preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter PI Publication...transcription", "Hahn", or other terms. ID Plasmid Description Gene/Insert Vector Type Promoter PI Publication...

  12. TALEN Engineering

    Type
    Collection
    ...laboratory for engineering TAL effectors, including designed TALENs, and assembly methods for REAL, REAL-Fast... engineering TAL effector repeat arrays of any desired length: the REAL (Restriction Enzyme And Ligation...for FLASH assembly. Links to additional pages describing these reagents are provided below: Joung Lab ...

  13. Resolute Plasmid Collection

    Type
    Collection
    ...proteomic interactions represent the framework that provides biological context. Assays monitoring impedance...sub-cloning into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled...solute carrier family of proteins. Library ID PI Description Human SLC Activation Library 132561 Superti-Furga...

  14. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...CoV-2), modification, or variant. ID Plasmid Description Mutations Industry PI Due to export control regulations...expressing mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro - Lentiviral...Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing...

  15. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two classes of base editors...protein (Mu- GAM). While many base editors are designed to work in a very narrow window proximal to the...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

  16. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...protein with many available isoforms and mutants provides a shared experience for the research community...site loop", or "subunit interface") ID Plasmid Description Gene/Insert Mutations Additional Resources MDH...Results in Student Learning Gains and Positive STEM Attitudes in CUREs. Callahan KP et al. CBE Life Sci Educ...

  17. Adenovirus Plasmids

    Type
    Collection
    ...strains for generating adenovirus. ID Strain Description PI 16398 BJ5183 Strain for recombination between... plasmids that have inserts. ID Plasmid Type Description PI 16400 pAdEasy-1 Adenoviral For recombining... be assembled in a one-tube reaction. ID Kit Description PI 1000000176 AdenoBuilder toolkit Plasmids contain...

  18. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ... are used in conjunction with the plasmid that encodes the viral genome (typically called cis -plasmid...plasmid). Helper Plasmid ID Plasmid Serotype Description PI 112867 pAdDeltaF6 All Helper plasmid for AAV...Packaging Service at Addgene ID Plasmid Capsid Description PI 112862 pAAV2/1 AAV1 AAV packaging plasmid,...

  19. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Mitochondria 87424 DSP-mEGFP AICSDP-9 mEGFP Desmoplakin Desmosomes 87425 ACTB-mEGFP AICSDP-15 mEGFP Beta-actin...human cell lines of their choice. For a full description of how to use these plasmids, check out Plasmids...

  20. Genetic Code Expansion

    Type
    Collection
    ...based upon four nucleotides, 64 codons, and 20 amino acids. Yet in the past two decades, biologists have...included. For any and all questions you have while designing, implementing, or troubleshooting GCE experiments...non-standard amino acid incorporation. ID Strain Description PI 48998 C321.ΔA all TAG sites changes to UAG...
Showing: 61 - 80 of 135 results