...( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a microbial nuclease system involved...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned scarlessly into the vector...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned into the CRISPR array. The...amplicons (Ran et al., Nature Protocols 2013). Individual human codon-optimized SpCas9 plasmids can be ...CMV, PX601 [#61591] ) or liver-specific tyroxine binding globulin (TBG, PX602 [#61593] ) promoter, and a...

...Link opens in a new window) is a public-private partnership of 13 members from industry and academia financed...Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression ...SLC Overexpression Library 213694 Superti-Furga Inducible barcoded overexpression library of human SLC proteins...

...method. This kit consists of 5 individually packaged plasmids and 3 individually packaged strains, which are...shipped as bacterial stabs at room temperature. Individual plasmids can be ordered from each plasmid page...following reagents (listed below and available individually or as an “OPEN Accessory Reagent Set”). These...

... ligand binding domain, which interacts with hormones or other molecules, and 2) a DNA binding domain,...the receptor to specific DNA sequences. Ligand binding triggers a conformational change in the receptor... an Addgene scientist. Nuclear Receptor Plasmids Find Plasmids by Gene Name Alternative Gene Names Gene...

...scientists. They are generated using standard methods individually optimized for each specific antibody in order...Innovation (IPI). For these antibodies, IPI will be indicated as the Depositing Lab on the antibody webpage.... cited in the Tissue Atlas (Link opens in a new window) . Western Blotting (WB) Proteins are extracted... or the Neuromab Protocols (Link opens in a new window) page. Storage and Shipping Stability Addgene does...

...Medicine Gene Vector Core (Link opens in a new window) have kindly deposited a series of lentiviral and adenoviral... this set of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've ... College of Medicine Gene Vector Core have also kindly provided this protocol detailing the process for...

...library contains 19973 barcodes to combinatorially index cells for single-cell analysis of clonal dynamics...library contains 4934 barcodes to combinatorially index cells for single-cell analysis of clonal dynamics...library contains 5737 barcodes to combinatorially index cells for single-cell analysis of clonal dynamics...Viral Service Suggestion Form (Link opens in a new window) . Please note this does not guarantee viral service...

...fluorescent protein (see this fusion protein guide to find the appropriate backbone). Then, by examining the...of interest. Browse or search the table below to find markers of general cellular organelles and structures...cell division. Browse or search the table below to find markers of cytoskeletal element. ID Plasmid Structure... desmosomes. Browse or search the table below to find markers for cellular junctions. ID Plasmid Structure...function properly. Browse or search the table below to find markers of secretory pathway components. ID Plasmid...

...viruses that are commonly used in research, and to find plasmids that can be used to engineer viruses for...Browse Viral Plasmids by Type Visit these links to find plasmids that can be used to engineer various types...because of the strong immunogenic response they induce. Some (oncolytic) adenoviruses are utilized for...

...LumiScarlet : LumiLuc and LumiScarlet are ATP-independent luciferases derived from teLuc that emit yellow...NanoLuc® and Venus fluorophore to Troponin C Ca 2+ binding domain. Nano-lanterns , Enhanced Nano-lanterns ...reference level of expression. Use this table to find plasmids expressing luciferase. We also offer ready-to-use...luciferase reporter for? Browse or use the search box to find a construct that contains your regulatory element...

...plasmid collections from depositors. To help you find what you're looking for, we've gathered our depositor...depositor collections on one page. We hope you find it helpful! You May Also Like: Plasmid Kits Plasmid ...Resource iPSC Neurodegenerative Disease Initiative (iNDI) Collection Rett Syndrome Plasmid Collection and...

...Research Foundation (NETRF) (Link opens in a new window) is to fund research to discover cures and more...grateful for the generous support of philanthropic individuals and foundations, who share in their vision to... Matthew Meyerson Dana-Farber Cancer Institute Finding the Causes of Small Intestinal Neuroendocrine Tumors...

...frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss...this page was generated with the help of Scott Lindner . Cut Fully functional CRISPR/Cas enzymes will ...

...version of the ZiFiT Targeter (Link opens in a new window) is available to enable users to identify both ...transcriptional activator target sites and then generates individual user-friendly graphical roadmaps for each target...provided below: Joung Lab REAL Assembly TALEN Kit Individual REAL TALE Repeat Plasmids TALEN Expression Vectors...

...pseudotyped with the SARS-CoV-2 Spike protein and you will find examples of each in Addgene's collection: HIV-based...appropriate packaging plasmids. Use the search bar to find a particular viral spike protein (MERS, CoV-1, CoV..., or variant. ID Plasmid Description Mutations Industry PI Due to export control regulations, MERS and...

.... CRISPR Resources : Browse depositor protocols, find software for gRNA design and deep sequencing analysis...CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together in CRISPR...CRISPR experiments. CRISPR Pooled Libraries : Find CRISPR libraries for knockout, activation, inhibition...

...frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss...homology to a genomic DNA locus and encodes a primer binding site and the desired edits on an RT template. ID...

...nuclease, the components of the array recognize individual nucleotides. For over ten years, zinc finger ...thymine, and NN targets guanine (though NN can also bind adenenine with lower specificity). With the template...also hosts web-based software to help scientists find potential TAL effector targets. The site, which ...

...of Pennsylvania Vector Core (Link opens in a new window) . Transfections are performed using the transfer...modified from Lock et al. (2014) (Link opens in a new window) ). Previous experience suggests that titers obtained...modified from Wang et al. (2018) (Link opens in a new window) ). Briefly, 293T cells are transduced with serial...

...have shown that engineered TALENs can be used to induce targeted mutations with high efficiency in zebrafish...TAAACGCCCTGATCTTCAgcgccgacggcctgcagcAGAGAGACAAGATGCGCA inhibitor of DNA binding 3 TAL3466 & TAL3467 TCAACATTGGATATCTATagagctttacagctagTTTATCCCAGTGTCCAGA...TGCAGCCGTGTTTCTACCcggacgaggatgacttCTACTTCTGCAGACCAGA NADPH oxidase, EF-hand calcium binding domain 5 TAL3496 & TAL3497 TGCCGCCAACTGGCTGAAacctccagatctggagCAGAACAAGCGCAAAA...