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Showing: 41 - 60 of 69 results

  1. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully functional ...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and 2. a specific edit close to the gRNA PAM site. When a repair template is present, the...

  2. CRISPR Plasmids and Resources

    Type
    Collection
    ...Empty gRNA Vectors Select an empty vector for your experiment based on a variety of factors. gRNAs gRNA... : Browse depositor protocols, find software for gRNA design and deep sequencing analysis, discover links...powerful tool for genome-wide screening. RNA Targeting gRNAs RNA Targeting Type VI CRISPR systems target RNA,...nuclease generates a DSB when expressed with two gRNAs. Browse Plasmids by Model Organism Bacteria C. elegans...

  3. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A catalytically inactive... break (DSB) at a specific sequence. Two unique gRNAs, binding ~15-25 bp apart, are required for dCas9...

  4. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To make targeted ... dead dCas9 to epigenetic modifiers. Design your gRNA to target a specific promoter or enhancer for your...

  5. CRISPR-Cas/RGN expression plasmids for human cells

    Type
    Collection
    ...RNA ( gRNA ) expression vector listed below ( MLM3636 ) can be used to construct customized gRNAs targeted...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts This page lists plasmids...available, web-based ZiFiT Targeter software. Specific gRNA expression plasmids can be built by cloning a pair...the links below: ID Plasmid 43860 MLM3636 (human gRNA expression vector) 42252 MLM3639 (Cas9 expression...

  6. FlyCRISPR

    Type
    Collection
    ...Browse CRISPR Cas9 gRNA plasmids for drosophila research from the O'Connor-Giles, Wildonger, and Harrison...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Harrison Lab - ...replacement by homologous recombination. For the sgRNA, vector pMB60 allows in vitro production of RNA ...template: example : Resources: Protocol for generating gRNAs 106.5 KB flyCRISPR website contains additional information...information CRISPR Optimal Target Finder Identifies gRNA target sequences from an input sequence and checks...

  7. CRISPR Plasmids - Base Edit

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two classes of base...Base editors are targeted to a specific locus by a gRNA, and they can convert cytidine to uridine within...

  8. Guide RNA Expression Plasmids for EGFP

    Type
    Collection
    ... the guide RNAs ( gRNAs ) used in these experiments are listed below. Note that gRNA produced from each... CRISPR Joung Lab CRISPR Plasmids EGFP gRNA CRISPR-Cas/RGN expression plasmids for EGFP You ...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The Joung Lab recently...

  9. Guide RNA Expression Plasmids for Endogenous Human Genes

    Type
    Collection
    ... the guide RNAs ( gRNAs ) used in these experiments are listed below. Note that gRNA produced from each... CRISPR Joung Lab CRISPR Plasmids Human gRNA CRISPR-Cas/RGN expression plasmids for Human genes...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The Joung Lab recently...

  10. CRISPR-Cas/RGN expression plasmids for Zebrafish

    Type
    Collection
    ...RNA ( gRNA ) expression vector listed below ( pDR274 ) can be used to construct customized gRNAs targeted...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts This page lists plasmids...available, web-based ZiFiT Targeter software. Specific gRNA expression plasmids can be built by cloning a pair...

  11. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type VI CRISPR systems...

  12. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts RNA-Guided Nucleases...consist of two components: a short ~100 nt guide RNA ( gRNA ) that uses 20 variable nts at its 5’ end to base...the target DNA. The Joung lab recently described gRNA and Cas9 expression vectors and validated the use...design of short oligonucleotides needed to construct gRNA expression vectors. More information about RGNs ...

  13. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type VI CRISPR systems...

  14. Boxem Lab CRISPR Plasmids

    Type
    Collection
    ...CRISPR Cas9 gRNA plasmids from Dr. Mike Boxem's lab....You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts We adapted the S....vectors for the delivery of the synthetic guide RNA (sgRNA). Each of the Cas9 vectors contains the Cas9 coding...with and without a C-terminal EGFP fusion. For the sgRNA, vector pMB60 allows in vitro production of RNA ...

  15. Bacterial Expression Systems

    Type
    Collection
    ...see here . pgRNA-bacteria 44251 CRISPR Stanley Qi Expression of customizable guide RNA (gRNA) for bacterial...can be combined with expression of a gRNA from a plasmid like pgRNA-bacteria to knock down endogenous gene...Marraffini Bacterial expression of Cas9 nuclease gRNA. For a more comprehensive list of our bacterial ...

  16. Plant Plasmids and Resources

    Type
    Collection
    ...assembly of one or more gRNA expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use in rice... different functional variants of Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR...Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots...

  17. Huang Lab CRISPR Plasmids

    Type
    Collection
    ...CRISPR Cas9 gRNA plasmids from Dr. Xingxu Huang's lab....You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts A human codon-optimized...

  18. Doudna Lab CRISPR Plasmids

    Type
    Collection
    ...CRISPR Cas9 gRNA plasmids from Dr. Jennifer Doudna's lab....You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Assorted Cas9 nucleases...

  19. Lentivirus Plasmids

    Type
    Collection
    ...plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table and search "lenti...
Showing: 41 - 60 of 69 results