RAS Clone Collections- RAS and RAS Pathway Constructs
(Kit # 1000000070, 1000000089 )

Depositing Lab: Dominic Esposito

The Esposito Lab has provided 2 kits for both Ras wild type and mutant constructs.

Kit #1000000070

The RAS Pathway 2.0 Clone Collection is a set of vectors for use with the Gateway® Cloning Platform (Life Technologies) to permit construction of RAS pathway gene expression vectors for in vitro and in vivo research. While these clones can be used for standard Gateway cloning, their utility is greatly enhanced with the use of the FNLCR Combinatorial Cloning Platform (CCP) which allows simplified construction of vectors with different elements (see Wall VE et al, 2014 for more information).

Kit #1000000089

This collection contains 61 wild type and mutant RAS genes as Gateway entry clones. It is comprised of wild type HRAS, NRAS, KRAS4a and KRAS4b gene plasmids as well as different mutant versions of each gene.

These kits will be sent as bacterial glycerol stocks in a 96-well plate format.

Description

Kit #1000000070

This collection contains 180 genes in the “RAS pathway version 2.0”, a crowdsourced pathway map of the RAS signaling pathway generated on the RASCentral website of the Frederick National Laboratory RAS Initiative. In each case, the primary splice variant was chosen to represent the most commonly found transcript by RNAseq data across all TCGA tumor samples. Many of these transcript variants are not available in cDNA or ORF libraries, or are available only in mutated forms. The sequences in this collection encode wild type proteins, and match the RefSeq data for the particular splice variants with the exception of known SNPs or cases in which the RefSeq data is not consistent with genome sequence data (see pathway map below).

Some uses of this system are:

• Construction of protein expression constructs with various fusion tags
• Generation of expression constructs with different promoters for in vivo expression
• Production of clones with fluorescent tags for localization experiments
• Generation of constructs for making mutant cell lines or transgenic animals
• Production of vectors for shRNA or miRNA delivery

Kit #1000000089

This collection contains 61 wild type and mutant RAS genes as Gateway entry clones. It is comprised of wild type HRAS, NRAS, KRAS4a and KRAS4b gene plasmids as well as different mutant versions of each gene.

Depositor Information

Kit #1000000070

RAS Pathway Clone Collection 2.0 Overview 709.2 KB

Annotated Plasmid Maps 1.1 MB

Gateway Recombinational Cloning

For more information on the Gateway system, please see:

DNA cloning using in vitro site-specific recombination. Hartley JL, Temple GF, Brasch MA. Genome Res.2000. Nov;10(11):1788-95. PubMed PMID 11076863

For more information on the FNLCR Combinatorial Cloning Platform (CCP):

Combinatorial assembly of clone libraries using site-specific recombination. Wall VE, Garvey LA, Mehalko JL, Procter LV, Esposito D. Methods Mol Biol. 2014;1116:193-208. doi: 10.1007/978-1-62703-764-8_14. PubMed PMID 24395366

How to Cite these Kits

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The RAS Clone Collection was a gift from Dominic Esposito (Addgene kit # ).”

RAS Pathway 2.0 Clone Collection - #1000000070

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right, as well as lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

	Spectinomycin
	Kanamycin

Inventory

Well	Plasmid	Resistance
Plate 1 / A / 1	R777-E001 Hs.AKT1	Spectinomycin
Plate 1 / A / 2	R777-E002 Hs.AKT1-nostop	Spectinomycin
Plate 1 / A / 3	R777-E003 Hs.AKT2	Spectinomycin
Plate 1 / A / 4	R777-E004 Hs.AKT2-nostop	Spectinomycin
Plate 1 / A / 5	R777-E005 Hs.AKT3	Spectinomycin
Plate 1 / A / 6	R777-E006 Hs.AKT3-nostop	Spectinomycin
Plate 1 / A / 7	R777-E007 Hs.ALK	Spectinomycin
Plate 1 / A / 8	R777-E008 Hs.ALK-nostop	Spectinomycin
Plate 1 / A / 9	R777-E009 Hs.ARAF	Spectinomycin
Plate 1 / A / 10	R777-E010 Hs.ARAF-nostop	Spectinomycin
Plate 1 / A / 11	R777-E011 Hs.ARHGAP35	Spectinomycin
Plate 1 / A / 12	R777-E012 Hs.ARHGAP35-nostop	Spectinomycin
Plate 1 / B / 1	R777-E013 Hs.ARHGEF2	Spectinomycin
Plate 1 / B / 2	R777-E014 Hs.ARHGEF2-nostop	Spectinomycin
Plate 1 / B / 3	R777-E015 Hs.BRAF	Spectinomycin
Plate 1 / B / 4	R777-E016 Hs.BRAF-nostop	Spectinomycin
Plate 1 / B / 5	R777-E017 Hs.CCND1	Spectinomycin
Plate 1 / B / 6	R777-E018 Hs.CCND1-nostop	Spectinomycin
Plate 1 / B / 7	R777-E019 Hs.CCND2	Spectinomycin
Plate 1 / B / 8	R777-E020 Hs.CCND2-nostop	Spectinomycin
Plate 1 / B / 9	R777-E021 Hs.CCND3	Spectinomycin
Plate 1 / B / 10	R777-E022 Hs.CCND3-nostop	Spectinomycin
Plate 1 / B / 11	R777-E023 Hs.CDK4	Spectinomycin
Plate 1 / B / 12	R777-E024 Hs.CDK4-nostop	Spectinomycin
Plate 1 / C / 1	R777-E025 Hs.CDK6	Spectinomycin
Plate 1 / C / 2	R777-E026 Hs.CDK6-nostop	Spectinomycin
Plate 1 / C / 3	R777-E027 Hs.CNKSR1	Spectinomycin
Plate 1 / C / 4	R777-E028 Hs.CNKSR1-nostop	Spectinomycin
Plate 1 / C / 5	R777-E029 Hs.CNKSR2	Spectinomycin
Plate 1 / C / 6	R777-E030 Hs.CNKSR2-nostop	Spectinomycin
Plate 1 / C / 7	R777-E031 Hs.CYTH2	Spectinomycin
Plate 1 / C / 8	R777-E032 Hs.CYTH2-nostop	Spectinomycin
Plate 1 / C / 9	R777-E033 Hs.DUSP1	Spectinomycin
Plate 1 / C / 10	R777-E034 Hs.DUSP1-nostop	Spectinomycin
Plate 1 / C / 11	R777-E035 Hs.DUSP2	Spectinomycin
Plate 1 / C / 12	R777-E036 Hs.DUSP2-nostop	Spectinomycin
Plate 1 / D / 1	R777-E037 Hs.DUSP3	Spectinomycin
Plate 1 / D / 2	R777-E038 Hs.DUSP3-nostop	Spectinomycin
Plate 1 / D / 3	R777-E039 Hs.DUSP4	Spectinomycin
Plate 1 / D / 4	R777-E040 Hs.DUSP4-nostop	Spectinomycin
Plate 1 / D / 5	R777-E041 Hs.DUSP5	Spectinomycin
Plate 1 / D / 6	R777-E042 Hs.DUSP5-nostop	Spectinomycin
Plate 1 / D / 7	R777-E043 Hs.DUSP6	Spectinomycin
Plate 1 / D / 8	R777-E044 Hs.DUSP6-nostop	Spectinomycin
Plate 1 / D / 9	R777-E045 Hs.E2F1	Spectinomycin
Plate 1 / D / 10	R777-E046 Hs.E2F1-nostop	Spectinomycin
Plate 1 / D / 11	R777-E047 Hs.E2F2	Spectinomycin
Plate 1 / D / 12	R777-E048 Hs.E2F2-nostop	Spectinomycin
Plate 1 / E / 1	R777-E049 Hs.E2F3	Spectinomycin
Plate 1 / E / 2	R777-E050 Hs.E2F3-nostop	Spectinomycin
Plate 1 / E / 3	R777-E051 Hs.ECT2	Spectinomycin
Plate 1 / E / 4	R777-E052 Hs.ECT2-nostop	Spectinomycin
Plate 1 / E / 5	R777-E053 Hs.EGFR	Spectinomycin
Plate 1 / E / 6	R777-E054 Hs.EGFR-nostop	Spectinomycin
Plate 1 / E / 7	R777-E055 Hs.EIF4EBP1	Spectinomycin
Plate 1 / E / 8	R777-E056 Hs.EIF4EBP1-nostop	Spectinomycin
Plate 1 / E / 9	R777-E057 Hs.EIF4EBP2	Spectinomycin
Plate 1 / E / 10	R777-E058 Hs.EIF4EBP2-nostop	Spectinomycin
Plate 1 / E / 11	R777-E059 Hs.EIF4EBP3	Spectinomycin
Plate 1 / E / 12	R777-E060 Hs.EIF4EBP3-nostop	Spectinomycin
Plate 1 / F / 1	R777-E061 Hs.ERBB2	Spectinomycin
Plate 1 / F / 2	R777-E062 Hs.ERBB2-nostop	Spectinomycin
Plate 1 / F / 3	R777-E063 Hs.ETS1	Spectinomycin
Plate 1 / F / 4	R777-E064 Hs.ETS1-nostop	Spectinomycin
Plate 1 / F / 5	R777-E065 Hs.ETS2	Spectinomycin
Plate 1 / F / 6	R777-E066 Hs.ETS2-nostop	Spectinomycin
Plate 1 / F / 7	R777-E067 Hs.EXOC1	Spectinomycin
Plate 1 / F / 8	R777-E068 Hs.EXOC1-nostop	Spectinomycin
Plate 1 / F / 9	R777-E069 Hs.EXOC2	Spectinomycin
Plate 1 / F / 10	R777-E070 Hs.EXOC2-nostop	Spectinomycin
Plate 1 / F / 11	R777-E071 Hs.EXOC3	Spectinomycin
Plate 1 / F / 12	R777-E072 Hs.EXOC3-nostop	Spectinomycin
Plate 1 / G / 1	R777-E073 Hs.EXOC4	Spectinomycin
Plate 1 / G / 2	R777-E074 Hs.EXOC4-nostop	Spectinomycin
Plate 1 / G / 3	R777-E075 Hs.EXOC5	Spectinomycin
Plate 1 / G / 4	R777-E076 Hs.EXOC5-nostop	Spectinomycin
Plate 1 / G / 5	R777-E077 Hs.EXOC6	Spectinomycin
Plate 1 / G / 6	R777-E078 Hs.EXOC6-nostop	Spectinomycin
Plate 1 / G / 7	R777-E079 Hs.EXOC7	Spectinomycin
Plate 1 / G / 8	R777-E080 Hs.EXOC7-nostop	Spectinomycin
Plate 1 / G / 9	R777-E081 Hs.EXOC8	Spectinomycin
Plate 1 / G / 10	R777-E082 Hs.EXOC8-nostop	Spectinomycin
Plate 1 / G / 11	R777-E083 Hs.FGFR1	Spectinomycin
Plate 1 / G / 12	R777-E084 Hs.FGFR1-nostop	Spectinomycin
Plate 1 / H / 1	R777-E085 Hs.FGFR2	Spectinomycin
Plate 1 / H / 2	R777-E086 Hs.FGFR2-nostop	Spectinomycin
Plate 1 / H / 3	R777-E087 Hs.FGFR3	Spectinomycin
Plate 1 / H / 4	R777-E088 Hs.FGFR3-nostop	Spectinomycin
Plate 1 / H / 5	R777-E089 Hs.FGFR4	Spectinomycin
Plate 1 / H / 6	R777-E090 Hs.FGFR4-nostop	Spectinomycin
Plate 1 / H / 7	R777-E091 Hs.FLT3	Spectinomycin
Plate 1 / H / 8	R777-E092 Hs.FLT3-nostop	Spectinomycin
Plate 1 / H / 9	R777-E093 Hs.FNTA	Spectinomycin
Plate 1 / H / 10	R777-E094 Hs.FNTA-nostop	Spectinomycin
Plate 1 / H / 11	R777-E095 Hs.FNTB	Spectinomycin
Plate 1 / H / 12	R777-E096 Hs.FNTB-nostop	Spectinomycin
Plate 2 / A / 1	R777-E193 Hs.PRKAA2	Spectinomycin
Plate 2 / A / 2	R777-E194 Hs.PRKAA2-nostop	Spectinomycin
Plate 2 / A / 3	R777-E195 Hs.PRKAB1	Spectinomycin
Plate 2 / A / 4	R777-E196 Hs.PRKAB1-nostop	Spectinomycin
Plate 2 / A / 5	R777-E197 Hs.PRKAB2	Spectinomycin
Plate 2 / A / 6	R777-E198 Hs.PRKAB2-nostop	Spectinomycin
Plate 2 / A / 7	R777-E199 Hs.PRKAG1	Spectinomycin
Plate 2 / A / 8	R777-E200 Hs.PRKAG1-nostop	Spectinomycin
Plate 2 / A / 9	R777-E201 Hs.PRKAG2	Spectinomycin
Plate 2 / A / 10	R777-E202 Hs.PRKAG2-nostop	Spectinomycin
Plate 2 / A / 11	R777-E203 Hs.PRKAG3	Spectinomycin
Plate 2 / A / 12	R777-E204 Hs.PRKAG3-nostop	Spectinomycin
Plate 2 / B / 1	R777-E205 Hs.PTEN	Spectinomycin
Plate 2 / B / 2	R777-E206 Hs.PTEN-nostop	Spectinomycin
Plate 2 / B / 3	R777-E207 Hs.RAC1	Spectinomycin
Plate 2 / B / 4	R777-E208 Hs.RAC1-nostop	Spectinomycin
Plate 2 / B / 5	R777-E209 Hs.RAC2	Spectinomycin
Plate 2 / B / 6	R777-E210 Hs.RAC2-nostop	Spectinomycin
Plate 2 / B / 7	R777-E211 Hs.RAC3	Spectinomycin
Plate 2 / B / 8	R777-E212 Hs.RAC3-nostop	Spectinomycin
Plate 2 / B / 9	R777-E213 Hs.RAF1	Spectinomycin
Plate 2 / B / 10	R777-E214 Hs.RAF1-nostop	Spectinomycin
Plate 2 / B / 11	R777-E215 Hs.RALA	Spectinomycin
Plate 2 / B / 12	R777-E216 Hs.RALA-nostop	Spectinomycin
Plate 2 / C / 1	R777-E217 Hs.RALB	Spectinomycin
Plate 2 / C / 2	R777-E218 Hs.RALB-nostop	Spectinomycin
Plate 2 / C / 3	R777-E219 Hs.RALBP1	Spectinomycin
Plate 2 / C / 4	R777-E220 Hs.RALBP1-nostop	Spectinomycin
Plate 2 / C / 5	R777-E221 Hs.RALGDS	Spectinomycin
Plate 2 / C / 6	R777-E222 Hs.RALGDS-nostop	Spectinomycin
Plate 2 / C / 7	R777-E223 Hs.RAPGEF1	Spectinomycin
Plate 2 / C / 8	R777-E224 Hs.RAPGEF1-nostop	Spectinomycin
Plate 2 / C / 9	R777-E225 Hs.RAPGEF2	Spectinomycin
Plate 2 / C / 10	R777-E226 Hs.RAPGEF2-nostop	Spectinomycin
Plate 2 / C / 11	R777-E227 Hs.RASA1	Spectinomycin
Plate 2 / C / 12	R777-E228 Hs.RASA1-nostop	Spectinomycin
Plate 2 / D / 1	R777-E229 Hs.RASA2	Spectinomycin
Plate 2 / D / 2	R777-E230 Hs.RASA2-nostop	Spectinomycin
Plate 2 / D / 3	R777-E231 Hs.RASA3	Spectinomycin
Plate 2 / D / 4	R777-E232 Hs.RASA3-nostop	Spectinomycin
Plate 2 / D / 5	R777-E233 Hs.RASAL1	Spectinomycin
Plate 2 / D / 6	R777-E234 Hs.RASAL1-nostop	Spectinomycin
Plate 2 / D / 7	R777-E235 Hs.RASAL2	Spectinomycin
Plate 2 / D / 8	R777-E236 Hs.RASAL2-nostop	Spectinomycin
Plate 2 / D / 9	R777-E237 Hs.RASAL3	Spectinomycin
Plate 2 / D / 10	R777-E238 Hs.RASAL3-nostop	Spectinomycin
Plate 2 / D / 11	R777-E239 Hs.RASGRF1	Spectinomycin
Plate 2 / D / 12	R777-E240 Hs.RASGRF1-nostop	Spectinomycin
Plate 2 / E / 1	R777-E241 Hs.RASGRF2	Spectinomycin
Plate 2 / E / 2	R777-E242 Hs.RASGRF2-nostop	Spectinomycin
Plate 2 / E / 3	R777-E243 Hs.RASGRP1	Spectinomycin
Plate 2 / E / 4	R777-E244 Hs.RASGRP1-nostop	Spectinomycin
Plate 2 / E / 5	R777-E245 Hs.RASGRP2	Spectinomycin
Plate 2 / E / 6	R777-E246 Hs.RASGRP2-nostop	Spectinomycin
Plate 2 / E / 7	R777-E247 Hs.RASGRP3	Spectinomycin
Plate 2 / E / 8	R777-E248 Hs.RASGRP3-nostop	Spectinomycin
Plate 2 / E / 9	R777-E249 Hs.RASGRP4	Spectinomycin
Plate 2 / E / 10	R777-E250 Hs.RASGRP4-nostop	Spectinomycin
Plate 2 / E / 11	R777-E251 Hs.RASSF1	Spectinomycin
Plate 2 / E / 12	R777-E252 Hs.RASSF1-nostop	Spectinomycin
Plate 2 / F / 1	R777-E253 Hs.RASSF10	Spectinomycin
Plate 2 / F / 2	R777-E254 Hs.RASSF10-nostop	Spectinomycin
Plate 2 / F / 3	R777-E255 Hs.RASSF2	Spectinomycin
Plate 2 / F / 4	R777-E256 Hs.RASSF2-nostop	Spectinomycin
Plate 2 / F / 5	R777-E257 Hs.RASSF3	Spectinomycin
Plate 2 / F / 6	R777-E258 Hs.RASSF3-nostop	Spectinomycin
Plate 2 / F / 7	R777-E259 Hs.RASSF4	Spectinomycin
Plate 2 / F / 8	R777-E260 Hs.RASSF4-nostop	Spectinomycin
Plate 2 / F / 9	R777-E261 Hs.RASSF5	Spectinomycin
Plate 2 / F / 10	R777-E262 Hs.RASSF5-nostop	Spectinomycin
Plate 2 / F / 11	R777-E263 Hs.RASSF6	Spectinomycin
Plate 2 / F / 12	R777-E264 Hs.RASSF6-nostop	Spectinomycin
Plate 2 / G / 1	R777-E265 Hs.RASSF7	Spectinomycin
Plate 2 / G / 2	R777-E266 Hs.RASSF7-nostop	Spectinomycin
Plate 2 / G / 3	R777-E267 Hs.RASSF8	Spectinomycin
Plate 2 / G / 4	R777-E268 Hs.RASSF8-nostop	Spectinomycin
Plate 2 / G / 5	R777-E269 Hs.RASSF9	Spectinomycin
Plate 2 / G / 6	R777-E270 Hs.RASSF9-nostop	Spectinomycin
Plate 2 / G / 7	R777-E271 Hs.RB1	Spectinomycin
Plate 2 / G / 8	R777-E272 Hs.RB1-nostop	Spectinomycin
Plate 2 / G / 9	R777-E273 Hs.RCE1	Spectinomycin
Plate 2 / G / 10	R777-E274 Hs.RCE1-nostop	Spectinomycin
Plate 2 / G / 11	R777-E275 Hs.RHEB	Spectinomycin
Plate 2 / G / 12	R777-E276 Hs.RHEB-nostop	Spectinomycin
Plate 2 / H / 1	R777-E277 Hs.RHOA	Spectinomycin
Plate 2 / H / 2	R777-E278 Hs.RHOA-nostop	Spectinomycin
Plate 2 / H / 3	R777-E279 Hs.RHOB	Spectinomycin
Plate 2 / H / 4	R777-E280 Hs.RHOB-nostop	Spectinomycin
Plate 2 / H / 5	R777-E281 Hs.RHOC	Spectinomycin
Plate 2 / H / 6	R777-E282 Hs.RHOC-nostop	Spectinomycin
Plate 2 / H / 7	R777-E283 Hs.ROCK1	Spectinomycin
Plate 2 / H / 8	R777-E284 Hs.ROCK1-nostop	Spectinomycin
Plate 2 / H / 9	R777-E285 Hs.ROCK2	Spectinomycin
Plate 2 / H / 10	R777-E286 Hs.ROCK2-nostop	Spectinomycin
Plate 2 / H / 11	R777-E287 Hs.ROS1	Spectinomycin
Plate 2 / H / 12	R777-E288 Hs.ROS1-nostop	Spectinomycin
Plate 3 / A / 1	R777-E097 Hs.FOS	Spectinomycin
Plate 3 / A / 2	R777-E098 Hs.FOS-nostop	Spectinomycin
Plate 3 / A / 3	R777-E099 Hs.GRB2	Spectinomycin
Plate 3 / A / 4	R777-E100 Hs.GRB2-nostop	Spectinomycin
Plate 3 / A / 5	R777-E101 Hs.HRAS	Spectinomycin
Plate 3 / A / 6	R777-E102 Hs.HRAS-nostop	Spectinomycin
Plate 3 / A / 7	R777-E103 Hs.ICMT	Spectinomycin
Plate 3 / A / 8	R777-E104 Hs.ICMT-nostop	Spectinomycin
Plate 3 / A / 9	R777-E105 Hs.INSR	Spectinomycin
Plate 3 / A / 10	R777-E106 Hs.INSR-nostop	Spectinomycin
Plate 3 / A / 11	R777-E107 Hs.INSRR	Spectinomycin
Plate 3 / A / 12	R777-E108 Hs.INSRR-nostop	Spectinomycin
Plate 3 / B / 1	R777-E109 Hs.IRS1	Spectinomycin
Plate 3 / B / 2	R777-E110 Hs.IRS1-nostop	Spectinomycin
Plate 3 / B / 3	R777-E111 Hs.IRS2	Spectinomycin
Plate 3 / B / 4	R777-E112 Hs.IRS2-nostop	Spectinomycin
Plate 3 / B / 5	R777-E113 Hs.JUN	Spectinomycin
Plate 3 / B / 6	R777-E114 Hs.JUN-nostop	Spectinomycin
Plate 3 / B / 7	R777-E115 Hs.KRAS	Spectinomycin
Plate 3 / B / 8	R777-E116 Hs.KRAS-nostop	Spectinomycin
Plate 3 / B / 9	R777-E117 Hs.KSR1	Spectinomycin
Plate 3 / B / 10	R777-E118 Hs.KSR1-nostop	Spectinomycin
Plate 3 / B / 11	R777-E119 Hs.KSR2	Spectinomycin
Plate 3 / B / 12	R777-E120 Hs.KSR2-nostop	Spectinomycin
Plate 3 / C / 1	R777-E121 Hs.MAP2K1	Spectinomycin
Plate 3 / C / 2	R777-E122 Hs.MAP2K1-nostop	Spectinomycin
Plate 3 / C / 3	R777-E123 Hs.MAP2K2	Spectinomycin
Plate 3 / C / 4	R777-E124 Hs.MAP2K2-nostop	Spectinomycin
Plate 3 / C / 5	R777-E125 Hs.MAPK1	Spectinomycin
Plate 3 / C / 6	R777-E126 Hs.MAPK1-nostop	Spectinomycin
Plate 3 / C / 7	R777-E127 Hs.MAPK3	Spectinomycin
Plate 3 / C / 8	R777-E128 Hs.MAPK3-nostop	Spectinomycin
Plate 3 / C / 9	R777-E129 Hs.MDM2	Spectinomycin
Plate 3 / C / 10	R777-E130 Hs.MDM2-nostop	Spectinomycin
Plate 3 / C / 11	R777-E131 Hs.MET	Spectinomycin
Plate 3 / C / 12	R777-E132 Hs.MET-nostop	Spectinomycin
Plate 3 / D / 1	R777-E133 Hs.MLST8	Spectinomycin
Plate 3 / D / 2	R777-E134 Hs.MLST8-nostop	Spectinomycin
Plate 3 / D / 3	R777-E135 Hs.MRAS	Spectinomycin
Plate 3 / D / 4	R777-E136 Hs.MRAS-nostop	Spectinomycin
Plate 3 / D / 5	R777-E137 Hs.MTOR	Spectinomycin
Plate 3 / D / 6	R777-E138 Hs.MTOR-nostop	Spectinomycin
Plate 3 / D / 7	R777-E139 Hs.NF1	Spectinomycin
Plate 3 / D / 8	R777-E140 Hs.NF1-nostop	Spectinomycin
Plate 3 / D / 9	R777-E141 Hs.NFE2L2	Spectinomycin
Plate 3 / D / 10	R777-E142 Hs.NFE2L2-nostop	Spectinomycin
Plate 3 / D / 11	R777-E143 Hs.NFKB1	Spectinomycin
Plate 3 / D / 12	R777-E144 Hs.NFKB1-nostop	Spectinomycin
Plate 3 / E / 1	R777-E145 Hs.NRAS	Spectinomycin
Plate 3 / E / 2	R777-E146 Hs.NRAS-nostop	Spectinomycin
Plate 3 / E / 3	R777-E147 Hs.PAK1	Spectinomycin
Plate 3 / E / 4	R777-E148 Hs.PAK1-nostop	Spectinomycin
Plate 3 / E / 5	R777-E149 Hs.PAK2	Spectinomycin
Plate 3 / E / 6	R777-E150 Hs.PAK2-nostop	Spectinomycin
Plate 3 / E / 7	R777-E151 Hs.PAK3	Kanamycin
Plate 3 / E / 8	R777-E152 Hs.PAK3-nostop	Spectinomycin
Plate 3 / E / 9	R777-E153 Hs.PAK4	Spectinomycin
Plate 3 / E / 10	R777-E154 Hs.PAK4-nostop	Spectinomycin
Plate 3 / E / 11	R777-E155 Hs.PDGFRA	Spectinomycin
Plate 3 / E / 12	R777-E156 Hs.PDGFRA-nostop	Spectinomycin
Plate 3 / F / 1	R777-E157 Hs.PDGFRB	Spectinomycin
Plate 3 / F / 2	R777-E158 Hs.PDGFRB-nostop	Spectinomycin
Plate 3 / F / 3	R777-E159 Hs.PDPK1	Spectinomycin
Plate 3 / F / 4	R777-E160 Hs.PDPK1-nostop	Spectinomycin
Plate 3 / F / 5	R777-E161 Hs.PEBP1	Spectinomycin
Plate 3 / F / 6	R777-E162 Hs.PEBP1-nostop	Spectinomycin
Plate 3 / F / 7	R777-E163 Hs.PIK3CA	Spectinomycin
Plate 3 / F / 8	R777-E164 Hs.PIK3CA-nostop	Spectinomycin
Plate 3 / F / 9	R777-E165 Hs.PIK3CB	Spectinomycin
Plate 3 / F / 10	R777-E166 Hs.PIK3CB-nostop	Spectinomycin
Plate 3 / F / 11	R777-E167 Hs.PIK3CD	Spectinomycin
Plate 3 / F / 12	R777-E168 Hs.PIK3CD-nostop	Spectinomycin
Plate 3 / G / 1	R777-E169 Hs.PIK3CG	Spectinomycin
Plate 3 / G / 2	R777-E170 Hs.PIK3CG-nostop	Spectinomycin
Plate 3 / G / 3	R777-E171 Hs.PIK3R1	Spectinomycin
Plate 3 / G / 4	R777-E172 Hs.PIK3R1-nostop	Spectinomycin
Plate 3 / G / 5	R777-E173 Hs.PIK3R2	Spectinomycin
Plate 3 / G / 6	R777-E174 Hs.PIK3R2-nostop	Spectinomycin
Plate 3 / G / 7	R777-E175 Hs.PIK3R3	Spectinomycin
Plate 3 / G / 8	R777-E176 Hs.PIK3R3-nostop	Spectinomycin
Plate 3 / G / 9	R777-E177 Hs.PIK3R4	Spectinomycin
Plate 3 / G / 10	R777-E178 Hs.PIK3R4-nostop	Spectinomycin
Plate 3 / G / 11	R777-E179 Hs.PIK3R5	Spectinomycin
Plate 3 / G / 12	R777-E180 Hs.PIK3R5-nostop	Spectinomycin
Plate 3 / H / 1	R777-E181 Hs.PIK3R6	Spectinomycin
Plate 3 / H / 2	R777-E182 Hs.PIK3R6-nostop	Spectinomycin
Plate 3 / H / 3	R777-E183 Hs.PIN1	Spectinomycin
Plate 3 / H / 4	R777-E184 Hs.PIN1-nostop	Spectinomycin
Plate 3 / H / 5	R777-E185 Hs.PLXNB1	Spectinomycin
Plate 3 / H / 6	R777-E186 Hs.PLXNB1-nostop	Spectinomycin
Plate 3 / H / 7	R777-E187 Hs.PPP1CA	Spectinomycin
Plate 3 / H / 8	R777-E188 Hs.PPP1CA-nostop	Spectinomycin
Plate 3 / H / 9	R777-E189 Hs.PREX2	Spectinomycin
Plate 3 / H / 10	R777-E190 Hs.PREX2-nostop	Spectinomycin
Plate 3 / H / 11	R777-E191 Hs.PRKAA1	Spectinomycin
Plate 3 / H / 12	R777-E192 Hs.PRKAA1-nostop	Spectinomycin
Plate 4 / A / 1	R777-E289 Hs.RPS6KA1	Spectinomycin
Plate 4 / A / 2	R777-E290 Hs.RPS6KA1-nostop	Spectinomycin
Plate 4 / A / 3	R777-E291 Hs.RPS6KA2	Spectinomycin
Plate 4 / A / 4	R777-E292 Hs.RPS6KA2-nostop	Spectinomycin
Plate 4 / A / 5	R777-E293 Hs.RPS6KA3	Spectinomycin
Plate 4 / A / 6	R777-E294 Hs.RPS6KA3-nostop	Spectinomycin
Plate 4 / A / 7	R777-E295 Hs.RPS6KA6	Spectinomycin
Plate 4 / A / 8	R777-E296 Hs.RPS6KA6-nostop	Spectinomycin
Plate 4 / A / 9	R777-E297 Hs.RPS6KB1	Spectinomycin
Plate 4 / A / 10	R777-E298 Hs.RPS6KB1-nostop	Spectinomycin
Plate 4 / A / 11	R777-E299 Hs.RPS6KB2	Spectinomycin
Plate 4 / A / 12	R777-E300 Hs.RPS6KB2-nostop	Spectinomycin
Plate 4 / B / 1	R777-E301 Hs.RPTOR	Spectinomycin
Plate 4 / B / 2	R777-E302 Hs.RPTOR-nostop	Spectinomycin
Plate 4 / B / 3	R777-E303 Hs.SAV1	Spectinomycin
Plate 4 / B / 4	R777-E304 Hs.SAV1-nostop	Spectinomycin
Plate 4 / B / 5	R777-E305 Hs.SCRIB	Spectinomycin
Plate 4 / B / 6	R777-E306 Hs.SCRIB-nostop	Spectinomycin
Plate 4 / B / 7	R777-E307 Hs.SHC1	Spectinomycin
Plate 4 / B / 8	R777-E308 Hs.SHC1-nostop	Spectinomycin
Plate 4 / B / 9	R777-E309 Hs.SHC2	Spectinomycin
Plate 4 / B / 10	R777-E310 Hs.SHC2-nostop	Spectinomycin
Plate 4 / B / 11	R777-E311 Hs.SHC3	Spectinomycin
Plate 4 / B / 12	R777-E312 Hs.SHC3-nostop	Spectinomycin
Plate 4 / C / 1	R777-E313 Hs.SHC4	Spectinomycin
Plate 4 / C / 2	R777-E314 Hs.SHC4-nostop	Spectinomycin
Plate 4 / C / 3	R777-E315 Hs.SHOC2	Spectinomycin
Plate 4 / C / 4	R777-E316 Hs.SHOC2-nostop	Spectinomycin
Plate 4 / C / 5	R777-E317 Hs.SOS1	Spectinomycin
Plate 4 / C / 6	R777-E318 Hs.SOS1-nostop	Spectinomycin
Plate 4 / C / 7	R777-E319 Hs.SOS2	Spectinomycin
Plate 4 / C / 8	R777-E320 Hs.SOS2-nostop	Spectinomycin
Plate 4 / C / 9	R777-E321 Hs.SPRED1	Spectinomycin
Plate 4 / C / 10	R777-E322 Hs.SPRED1-nostop	Spectinomycin
Plate 4 / C / 11	R777-E323 Hs.SPRED2	Spectinomycin
Plate 4 / C / 12	R777-E324 Hs.SPRED2-nostop	Spectinomycin
Plate 4 / D / 1	R777-E325 Hs.SPRED3	Spectinomycin
Plate 4 / D / 2	R777-E326 Hs.SPRED3-nostop	Spectinomycin
Plate 4 / D / 3	R777-E327 Hs.SPRY1	Spectinomycin
Plate 4 / D / 4	R777-E328 Hs.SPRY1-nostop	Spectinomycin
Plate 4 / D / 5	R777-E329 Hs.SPRY2	Spectinomycin
Plate 4 / D / 6	R777-E330 Hs.SPRY2-nostop	Spectinomycin
Plate 4 / D / 7	R777-E331 Hs.SPRY3	Spectinomycin
Plate 4 / D / 8	R777-E332 Hs.SPRY3-nostop	Spectinomycin
Plate 4 / D / 9	R777-E334 Hs.SPRY4-nostop	Spectinomycin
Plate 4 / D / 10	R777-E333 Hs.SPRY4	Spectinomycin
Plate 4 / D / 11	R777-E335 Hs.STK11	Spectinomycin
Plate 4 / D / 12	R777-E336 Hs.STK11-nostop	Spectinomycin
Plate 4 / E / 1	R777-E337 Hs.STK3	Spectinomycin
Plate 4 / E / 2	R777-E338 Hs.STK3-nostop	Spectinomycin
Plate 4 / E / 3	R777-E339 Hs.STK4	Spectinomycin
Plate 4 / E / 4	R777-E340 Hs.STK4-nostop	Spectinomycin
Plate 4 / E / 5	R777-E341 Hs.TBK1	Spectinomycin
Plate 4 / E / 6	R777-E342 Hs.TBK1-nostop	Spectinomycin
Plate 4 / E / 7	R777-E343 Hs.TFDP1	Spectinomycin
Plate 4 / E / 8	R777-E344 Hs.TFDP1-nostop	Spectinomycin
Plate 4 / E / 9	R777-E345 Hs.TFDP2	Spectinomycin
Plate 4 / E / 10	R777-E346 Hs.TFDP2-nostop	Spectinomycin
Plate 4 / E / 11	R777-E347 Hs.TIAM1	Spectinomycin
Plate 4 / E / 12	R777-E348 Hs.TIAM1-nostop	Spectinomycin
Plate 4 / F / 1	R777-E349 Hs.TIAM2	Spectinomycin
Plate 4 / F / 2	R777-E350 Hs.TIAM2-nostop	Spectinomycin
Plate 4 / F / 3	R777-E351 Hs.TP53	Spectinomycin
Plate 4 / F / 4	R777-E352 Hs.TP53-nostop	Spectinomycin
Plate 4 / F / 5	R777-E353 Hs.TSC1	Spectinomycin
Plate 4 / F / 6	R777-E354 Hs.TSC1-nostop	Spectinomycin
Plate 4 / F / 7	R777-E355 Hs.TSC2	Spectinomycin
Plate 4 / F / 8	R777-E356 Hs.TSC2-nostop	Spectinomycin
Plate 4 / F / 9	R777-E357 Hs.VAV1	Spectinomycin
Plate 4 / F / 10	R777-E358 Hs.VAV1-nostop	Spectinomycin
Plate 4 / F / 11	R777-E359 Hs.YAP1	Spectinomycin
Plate 4 / F / 12	R777-E360 Hs.YAP1-nostop	Spectinomycin

Data calculated @ 2025-09-04

RAS mutant clone collection - #1000000089

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Spectinomycin

Inventory

Well	Plasmid	Resistance
A / 1	Hs.KRAS4b	Spectinomycin
A / 2	Hs.KRAS4b G12C	Spectinomycin
A / 3	Hs.KRAS4b G12D	Spectinomycin
A / 4	Hs.KRAS4b G12V	Spectinomycin
A / 5	Hs.KRAS4b G13D	Spectinomycin
A / 6	Hs.KRAS4b Q61L	Spectinomycin
A / 7	Hs.KRAS4b Q61R	Spectinomycin
A / 8	Hs.KRAS4b S181A	Spectinomycin
A / 9	Hs.KRAS4b S181D	Spectinomycin
A / 10	Hs.KRAS4b S181E	Spectinomycin
A / 11	Hs.KRAS4b C185S	Spectinomycin
A / 12	Hs.KRAS4b Q61H	Spectinomycin
B / 1	Hs.KRAS4b M188L	Spectinomycin
B / 2	Hs.KRAS4b G12A	Spectinomycin
B / 3	Hs.KRAS4b G12R	Spectinomycin
B / 4	Hs.KRAS4b G12S	Spectinomycin
B / 5	Hs.KRAS4b G13C	Spectinomycin
B / 6	Hs.KRAS4b K117N	Spectinomycin
B / 7	Hs.KRAS4b A146V	Spectinomycin
B / 8	Hs.KRAS4b R68S	Spectinomycin
B / 9	Hs.KRAS4b G12F	Spectinomycin
B / 10	Hs.KRAS4b A146T	Spectinomycin
B / 11	Hs.KRAS4b Y64A	Spectinomycin
B / 12	Hs.KRAS4b K104A	Spectinomycin
C / 1	Hs.KRAS4b K104Q	Spectinomycin
C / 2	Hs.KRAS4b K147L	Spectinomycin
C / 3	Hs.KRAS4b K147Q	Spectinomycin
C / 4	Hs.KRAS4b S17N	Spectinomycin
C / 5	Hs.KRAS4b T35S	Spectinomycin
C / 6	Hs.KRAS4b E37G	Spectinomycin
C / 7	Hs.KRAS4b Y40C	Spectinomycin
C / 8	Hs.KRAS4b M72C	Spectinomycin
C / 9	Hs.KRAS4b Y40F	Spectinomycin
C / 10	Hs.KRAS4b R73E	Spectinomycin
C / 11	Hs.KRAS4b R73K	Spectinomycin
C / 12	Hs.KRAS4b Q61K	Spectinomycin
D / 1	Hs.KRAS4b G12D/C185S	Spectinomycin
D / 2	Hs.KRAS4a	Spectinomycin
D / 3	Hs.KRAS4a G12C	Spectinomycin
D / 4	Hs.KRAS4a G12D	Spectinomycin
D / 5	Hs.KRAS4a G12V	Spectinomycin
D / 6	Hs.KRAS4a G13D	Spectinomycin
D / 7	Hs.KRAS4a Q61L	Spectinomycin
D / 8	Hs.KRAS4a Q61R	Spectinomycin
D / 9	Hs.NRAS	Spectinomycin
D / 10	Hs.NRAS G12V	Spectinomycin
D / 11	Hs.NRAS G12C	Spectinomycin
D / 12	Hs.NRAS G12D	Spectinomycin
E / 1	Hs.NRAS G13D	Spectinomycin
E / 2	Hs.NRAS Q61L	Spectinomycin
E / 3	Hs.NRAS Q61R	Spectinomycin
E / 4	Hs.NRAS Q61K	Spectinomycin
E / 5	Hs.HRAS	Spectinomycin
E / 6	Hs.HRAS G12C	Spectinomycin
E / 7	Hs.HRAS G12D	Spectinomycin
E / 8	Hs.HRAS G12V	Spectinomycin
E / 9	Hs.HRAS G13D	Spectinomycin
E / 10	Hs.HRAS Q61L	Spectinomycin
E / 11	Hs.HRAS Q61K	Spectinomycin
E / 12	Hs.HRAS Q61R	Spectinomycin
F / 1	Hs.HRAS C186S	Spectinomycin

Data calculated @ 2025-09-04