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We narrowed to 3,111 results for: GAI

Showing: 1 - 50 of 3111 results

  4. TRUPATH Triple Gai1

    Plasmid
    #196048
    Purpose
    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196048-plasmid-map-sequence-id-385883

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  5. TRUPATH Triple Gai3

    Plasmid
    #196050
    Purpose
    Encodes a G alpha subunit (GNAl3) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai3-RLuc8 (GNAI3 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196050-plasmid-map-sequence-id-385864

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  6. TRUPATH Triple Gai2

    Plasmid
    #196049
    Purpose
    Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai2-RLuc8 (GNAI2 Human)
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196049-plasmid-map-sequence-id-385863

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  24. pSBtet-Bla/PAG1-TurboID-GAI

    Plasmid
    #198217
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with human PAG1, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling
    Depositor
    Eric Davis
    Insert
    PAG1 (PAG1 Human)
    Use
    Transposon
    Tags
    3’ part of TurboID, starting with G257 and amino …
    Expression
    Mammalian
    Mutation
    None
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198217-plasmid-map-sequence-id-389406

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  26. pSBtet-Bla/mCardinal-TurboID-GAI

    Plasmid
    #198218
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with mCardinal, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling
    Depositor
    Eric Davis
    Insert
    mCardinal
    Use
    Transposon
    Tags
    3’ part of TurboID, starting with G257 and amino …
    Expression
    Mammalian
    Mutation
    None
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198218-plasmid-map-sequence-id-389373

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  43. pIRES-mCerulean-GID1-KRas/N12-KRas/13C(Q61L)-GAI1-92-mVenus-CAAX

    Plasmid
    #214286
    Purpose
    Express split-KRas fragments that are fused with CIDs and FPs
    Depositor
    Cliff Stains
    Article
    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202
    Inserts
    Split-KRas fragments fused with CIDs and FPs (KRAS Human)
    Split-KRas fragments fused with CIDs and FPs (KRAS Human)
    Use
    Tags
    mCerulean (N terminal on insert 1) and mVenus (on…
    Expression
    Mammalian
    Mutation
    KRas-Q61L
    Promoter
    CMV
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214286-plasmid-map-sequence-id-422240

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  44. pIRES-mCerulean-GID1-KRas/N12-KRas/13C(S17N)-GAI1-92-mVenus-CAAX

    Plasmid
    #214287
    Purpose
    Express split-KRas fragments that are fused with CIDs and FPs
    Depositor
    Cliff Stains
    Article
    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202
    Inserts
    Split-KRas fragments fused with CIDs and FPs (KRAS Human)
    Split-KRas fragments fused with CIDs and FPs (KRAS Human)
    Use
    Tags
    mCerulean (on insert 1) and mVenus (on insert 2)
    Expression
    Mammalian
    Mutation
    KRas-S17N
    Promoter
    CMV
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214287-plasmid-map-sequence-id-422239

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  46. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

    Plasmid
    #84244
    Purpose
    Expresses GA-inducible VPR-Sa dCas9
    Depositor
    Stanley Qi
    Article
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Inserts
    GAI-tagBFP-Sa dCas9
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …
    Expression
    Mammalian
    Mutation
    D10A, N580A and Synonymous mutation in PYL1 to re…
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    84244-plasmid-map-sequence-id-160887

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  47. pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid
    #84261
    Purpose
    Expresses AND gate ABA/GA-inducible VPR-Sp dCas9
    Depositor
    Stanley Qi
    Article
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Inserts
    GAI-tagBFP-Sp dCas9
    PYL1-VPR-p2a-GID1-ABI
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, GAI, GID1, HA tag, PYL1, p2a, …
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and PGK
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    84261-plasmid-map-sequence-id-160892

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  48. pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

    Plasmid
    #84257
    Purpose
    Expresses Sp dCas9 and GA-inducible VPR for OR gate/diametric regulation
    Depositor
    Stanley Qi
    Article
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Inserts
    GAI-tagBFP-Sp dCas9-ABI
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and PGK
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    84257-plasmid-map-sequence-id-160891

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  49. pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

    Plasmid
    #106895
    Purpose
    Expresses Sp dCas9 and GA-inducible KRAB for NOR gate/diametric regulation
    Depositor
    Stanley Qi
    Article
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Inserts
    GAI-tagBFP-Sp dCas9-ABI
    GID1-KRAB
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and PGK
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    106895-plasmid-map-sequence-id-201653

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Showing: 1 - 50 of 3111 results