We narrowed to 7,871 results for: 199

1. BA31_pU.CAG.SaCas9-D10A.rBGpA

   Plasmid

   #199251

   Purpose

   Expression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   SaCas9.D10A nickase
   

   Use

   CRISPR

   Tags

   3XHA epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUREF-EX

   Plasmid

   #199331

   Purpose

   A pEF-BOS-EX-derived expression vector that contains an SV40 promoter-driven puromycin resistance gene

   Depositor

   Rikiro Fukunaga

   Article

   Fujii et al Genes Cells. 2023 Mar 2. doi: 10.1111/gt

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Mito-LOV-Turbo-V5

   Plasmid

   #199666

   Purpose

   expresses LOV-Turbo in the mammalian mitochondria, lentiviral vector

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   Lentiviral

   Tags

   COX4I1 mito targeting sequence and V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Anti-Ankyrin-G [N106/36R-2b]

   Plasmid

   #199407

   Purpose

   Mammalian expression plasmid of Anti-Ankyrin-G [N106/36R-2b] (Human). Derived from hybridoma N106/36.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Anti-Ankyrin-G (Human) recombinant mouse monoclonal antibody (ANK3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. AZ64_pE.DonorCLYBL.TS

   Plasmid

   #199228

   Purpose

   Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

   Insert

   Expression unit for EGFP reporter
   

   Use

   Gene targeting donor plasmid

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human PGK1 gene promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2

   Plasmid

   #199482

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   Gal4vp16/4xnrUAS-mKate2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-6xHis-GFP(0)

   Plasmid

   #199166

   Purpose

   used to express a neutral GFP variant in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(0)
   

   Use

   Tags

   MGHHHHHHGGA

   Expression

   Mutation

   E16R, I138R, D207R, N222K, L246R

   Promoter

   T7

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCS

   Plasmid

   #199351

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagBFP-P2A-ALFA

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCBL101.1

   Plasmid

   #199724

   Purpose

   A pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene. BsaI site was removed.

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   Multiple Cloning Site, NPTII
   

   Use

   Tags

   Expression

   Plant

   Mutation

   "GAGACC" to "GATACC"

   Promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSA_0_mKate2_synCoTC

    Plasmid

    #199469

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    mKate2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRep

    Plasmid

    #199599

    Purpose

    Expresses AAV2 rep gene; accessory plasmid for the pCMV-Cap SSM library.

    Depositor

    George Church

    Article

    Ogden et al Science. 2019 Nov 29;366(6469):1139-1143

    Insert

    AAV2 rep
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. TS MS-Sensor

    Plasmid

    #199788

    Purpose

    Synthetic gene circuit

    Depositor

    Timothy Lu

    Article

    Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

    Insert

    TS MS-Sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-cMSP1D1

    Plasmid

    #199768

    Purpose

    Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSP1D1
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    external His-Tags flanking the C and N termini

    Promoter

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTol2_LSEC:Cas9green; erbb2_gRNA171

    Plasmid

    #199338

    Purpose

    pDEL135; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous erbb2 sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    LSEC
    Cas9
    erbb2 sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPRi

    Plasmid

    #199808

    Purpose

    vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

    Depositor

    Steve Salipante

    Article

    Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

    Inserts

    dCas9
    sgRNA with GFP cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2

    Plasmid

    #199481

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    Gal4vp16/4xnrUAS-mKate2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV_CMV_CCR6-Nanoluc-V5

    Plasmid

    #199672

    Purpose

    expresses CCR6-NanoLuc-V5 in mammalian cells, AAV vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    CCR6 (CCR6 Human)
    

    Use

    AAV

    Tags

    NanoLuc and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-D2s-L-Venus

    Plasmid

    #19966

    Depositor

    Jonathan Javitch

    Article

    Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

    Insert

    D2 short variant-monomeric Venus (DRD2 Human, Aequorea Victoria)
    

    Use

    Tags

    Flag and monomeric Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHJL1

    Plasmid

    #199376

    Purpose

    Encodes dual cistronic cytosolic GCaMP6s and mScarlet-I optimized for C. elegans expression

    Depositor

    Hang Lu

    Insert

    GCaMP6s::SL2::mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    no mutations

    Promoter

    Available since

    April 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. TT MS-Sensor

    Plasmid

    #199789

    Purpose

    Synthetic gene circuit

    Depositor

    Timothy Lu

    Article

    Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

    Insert

    TT MS-Sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge