We narrowed to 17,417 results for: URE

1. BPK2139

   Plasmid

   #65776

   Purpose

   Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEMPTY::sgRNA2

   Plasmid

   #165459

   Purpose

   Escherichia coli – Staphylococcus aureus shuttle vector for plasmid curing in Gram-positive bacteria

   Depositor

   Inigo Lasa

   Article

   Dorado-Morales et al mBio. 2021 Feb 23;12(1). pii: mBio.03094

   Insert

   sgRNA2
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   SP01

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJB38-NWMN2930

   Plasmid

   #84457

   Purpose

   pJB38 plasmid containing the S.aureus Newman genetic region between genes 29 and 30

   Depositor

   Reindert Nijland

   Article

   de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

   Insert

   Staphylococcus aureus NWMN genomic region NWMN29-30
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. LacD_L165E/L275S_pET21a

   Plasmid

   #29788

   Depositor

   SeWon Suh

   Article

   Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

   Insert

   Tagatose 1,6-diphosphate aldolase
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   L165E and L275S

   Promoter

   Available since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pLVX-Flag-MIB1-IRES-Puro

   Plasmid

   #140240

   Purpose

   Lentiviral vector expressing flag-tagged MIB1

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Insert

   Flag-MIB1 (MIB1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. shRNA_circHUWE1_1

   Plasmid

   #215236

   Purpose

   Supression of shcircHUWE1(19,20)_1 expression

   Depositor

   Sefi Rosenbluh

   Article

   Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

   Insert

   circHUWE1 shRNA 1 (HUWE1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. shRNA_circHUWE1_2

   Plasmid

   #215235

   Purpose

   Supression of shcircHUWE1(19,20)_2 expression

   Depositor

   Sefi Rosenbluh

   Article

   Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

   Insert

   circHUWE1 shRNA 2 (HUWE1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST_FLAG-HUWE1_UBA/UIM/ UBM-mut

   Plasmid

   #187154

   Purpose

   mammalian cell expression of FLAG-HUWE1 L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Tags

   1xFLAG

   Expression

   Mammalian

   Mutation

   L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

   Promoter

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCIneoRL-BCL2 3'UTR-mir200c-mut

   Plasmid

   #84598

   Purpose

   Renilla Luciferase reporter assay for BCL2 3'UTR with mutations on miR-200c binding site

   Depositor

   Yanan Yang

   Article

   Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

   Insert

   BCL2 3'UTR (UTS2R Human)
   

   Use

   Luciferase

   Tags

   Renilla Luciferase

   Expression

   Mutation

   Mutation on miR-200c binding site on BCL2 3'…

   Promoter

   CMV

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pCIneoRL-TOB1 3'UTR-mir200c-mut

    Plasmid

    #84590

    Purpose

    Renilla Luciferase reporter assay for TOB1 3'UTR with mutations on miR-200c binding site

    Depositor

    Yanan Yang

    Article

    Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

    Insert

    TOB1 3'UTR (UTS2R Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mutation

    Mutation on miR-200c binding site on TOB1 3'…

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

11. dCAS9-VP64_GFP

    Plasmid

    #61422

    Purpose

    Expresses dCAS9-VP64 activator with 2A GFP

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Concentrated Lentiviral Prep

    Insert

    dCAS9(D10A,H840A)-VP64_2A_GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    EF1A

    Available since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. sgRNA(MS2) cloning backbone

    Plasmid

    #61424

    Purpose

    sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti dCAS-VP64_Blast

    Plasmid

    #61425

    Purpose

    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Lentiviral Prep

    Insert

    dCAS9(D10A, N863A)-VP64_2A_Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1A

    Available since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti sgRNA(MS2)_zeo backbone

    Plasmid

    #61427

    Purpose

    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti sgRNA(MS2)_puro backbone

    Plasmid

    #73795

    Purpose

    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV CMV-dSaCas9-KRAB-bGHpA

    Plasmid

    #106219

    Purpose

    AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA

    Plasmid

    #61594

    Purpose

    A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Insert

    hSaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV hUbC-dSaCas9-KRAB-T2A-PuroR

    Plasmid

    #106249

    Purpose

    Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB T2A PuroR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA-NLS and NLS-KRAB

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    hUbC

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. DTECT - Plasmid for Standard Curve

    Plasmid

    #139333

    Purpose

    This plasmid is used to generate the unique standard curve required to quantify the capture of genomic signature using DTECT

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    DTECT ligation product
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. MS2-P65-HSF1_GFP

    Plasmid

    #61423

    Purpose

    Expresses the MS2-P65-HSF1 activator helper complex with a 2A GFP. 3rd generation lentiviral vector.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Insert

    MS2(N55K)-P65-HSF1_2A_GFP (HSF1 Synthetic, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1A

    Available since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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