We narrowed to 1,145 results for: cas12

1. p15a-AsCas12f-apmR

   Plasmid

   #171610

   Purpose

   AsCas12f1-based K. pneumoniae genome editing

   Depositor

   Quanjiang Ji

   Article

   Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

   Insert

   AsCas12f1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-SpaCas12f1-gRNA_MS13

   Plasmid

   #190877

   Purpose

   The plasmid of CRISPR/SpaCas12f1(Syntrophomonas palmitatica Cas12f1) for genome editing in human cells

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Rep. 2022 Sep 27;40(13):111418. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. 6xHis-MBP-huBoCas12a

   Plasmid

   #174673

   Purpose

   Expresses 6xHis-MBP tagged BoCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huBoCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ230-D156R (ttLbCas12a)

   Plasmid

   #195366

   Purpose

   A temperature-tolerant LbCas12a mutant (D156R), also known as ttLbCas12a

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

   Insert

   LbCas12a-D156R (ttLbCas12a)
   

   Use

   CRISPR; Gateway compatible lbcas12a-d156r (ttlbca…

   Tags

   Expression

   Plant

   Mutation

   Promoter

   ZmUBI1

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6xHis-MBP-huPb2Cas12a

   Plasmid

   #174686

   Purpose

   Expresses 6xHis-MBP tagged Pb2Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huPb2Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. 6xHis-MBP-BrCas12b

   Plasmid

   #182276

   Purpose

   Expresses recombinant BrCas12b

   Depositor

   Piyush Jain

   Article

   Nguyen et al EBioMedicine. 2022 Mar;77:103926. doi: 1

   Insert

   BrCas12b
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, T7, Thrombin site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-HkCas12a-2AeGFP

   Plasmid

   #123634

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

   Insert

   HkCas12a
   

   Use

   Tags

   3xHA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZ149-pcDNA3-BhCas12b_v4

   Plasmid

   #122446

   Purpose

   Expresses BhCas12b_v4 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

   Insert

   CMV-NLS-BhCas12b_v4-NLS-3xHA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-mH6-Cas12i1

   Plasmid

   #120882

   Purpose

   Expresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12i1
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lac

   Available since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-SUMO-AsCas12f

    Plasmid

    #171612

    Purpose

    AsCas12f1 heterologous expression plasmid

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

    Insert

    AsCas12f1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJCC_076 AsCas12a R1226A

    Plasmid

    #171669

    Purpose

    For bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    AsCas12a R1226A
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed arginine 1226 to alanine

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AsCas12a-2C-NLS in pCSDest

    Plasmid

    #126637

    Purpose

    Expresses AsCas12a-2C-NLS in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    AsCas12a-2C-NLS
    

    Use

    CRISPR

    Tags

    3xHuman influenza hemagglutinin (HA)-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94 promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. plenti-As-Cas12a-2xNLS

    Plasmid

    #155047

    Purpose

    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged _Acidaminococcus sp. BV3L6 (As)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    AsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS, Myc-tag and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-AsCas12a-2AeGFP

    Plasmid

    #123638

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    AsCas12a
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-AaCas12b-2AeGFP

    Plasmid

    #121945

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMBP-LbCas12a D832A

    Plasmid

    #113517

    Purpose

    Expresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nuclease

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    LbCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    D832A

    Promoter

    T7

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZ143-pcDNA3-BvCas12b

    Plasmid

    #122445

    Purpose

    Expresses BvCas12b in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    CMV-NLS-BvCas12b-NLS-3xHA
    

    Use

    Tags

    3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCJH009_proD_Cas12c-sgRNA-GFP_CAM

    Plasmid

    #183070

    Purpose

    Plasmid expressing Cas12c sgRNA targeting GFP (1) for in vivo interference assay in bacteria.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c single guide RNA targeting GFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-AsCas12f-v4.1

    Plasmid

    #204636

    Purpose

    Expression of AsCas12f v4.1 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    AsCas12f-v4.1
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    D169K, N199K, N328G, D364R

    Promoter

    CMV

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-PxCas12a-2AeGFP

    Plasmid

    #123637

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    PxCas12a
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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