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Plasmid#174571PurposeHuman HA-tagged SOCS1 expression (N-term. tag) (CMV prom.)DepositorInsertHA-SOCS1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-EglN2-pLenti6
Plasmid#36950DepositorInsertEglN2 (EGLN2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationWildtypePromoterCMVAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
FLAG-EglN3-pLenti6
Plasmid#36951DepositorInsertEglN3 (EGLN3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationWildtypePromoterCMVAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET21a-TEX264-deltaLRR-His6
Plasmid#220215PurposeBacterial expression of TEX264, 2-25 residues (LRR) truncated, C-terminal His6 tagDepositorInsertTEX264 (TEX264 Human)
UseTagsHis6ExpressionBacterialMutationc.4-75del / p.2-24delPromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-INTS3-FL
Plasmid#128415PurposeExpress INTS3 full-length in E. coli with a GST tag (N-terminal)DepositorInsertINTS3 (INTS3 Human)
UseTagsGSTExpressionBacterialMutationfull-lengthPromotertac promoterAvailable sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTwist-WIV1-CoV
Plasmid#164438PurposeEncodes WIV1-CoV Spike Protein for pseudovirus productionDepositorInsertWIV1-CoV Spike
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
iBE3 CLYBL
Plasmid#174569Purposedoxycycline inducible BE3 expression for human CLYBL locus knock-inDepositorInsertBE3
UseTagsExpressionMammalianMutationPromotertet ONAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-INTS3-N
Plasmid#128416PurposeExpress INTS3 N-termini (1-513 aa) in E. coli with a GST tag (N-terminal)DepositorInsertINTS3 (INTS3 Human)
UseTagsGSTExpressionBacterialMutationN-terminal region (1–513 aa)Promotertac promoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Hygro-eGFP
Plasmid#99375PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-eGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG
Plasmid#155305PurposeLentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.DepositorInsertmCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
UseLentiviralTags2xNLS-3xFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-GFP
Plasmid#181906PurposeFluorescently tagged dead Cas9DepositorInsertdCas9
UseCRISPRTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Hygro-mTagBFP2
Plasmid#99374PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-mTagBFP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Hygro-dTomato
Plasmid#99376PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-dTomato
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP
Plasmid#155307PurposeRfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFPDepositorInsertRfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP
UseLentiviralTagsExpressionMammalianMutationPromoterhU6, EF1aAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGFP-hPMLIII
Plasmid#181903PurposeFluorescently tagged PMLIIIDepositorInserthPMLIII (PML Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
OA-1136J
Plasmid#153023PurposeExpress His-MBP-TEV-CasRx recombinant proteinDepositorInsertCasRx
UseTagsHis-MBP-TEVExpressionBacterialMutationPromoterT7Available sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
WT A1-LCD +NLS
Plasmid#178658PurposeBacterial expression of N-terminally 6His tagged WT A1-LCD (LCD: low-complexity domain, aa 186-320)DepositorInsertWT A1-LCD +NLS (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-monSTIM1
Plasmid#161795PurposeMammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)DepositorInsertMonSTIM1 (STIM1 Human, Mustard Weed)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Hygro-iRFP670
Plasmid#99377PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A iRFP670 from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-iRFP670
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE-BI-aaAFB2
Plasmid#207839PurposeInducible AFB2 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cellsDepositorInsertsatAFB2
mCherry
miniIAA7
VHH-GFP4
UseTags3X FLAG-Tag and weak NLSExpressionMammalianMutationAll K mutated to RPromoterTRE3G BI promoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only