Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 12,310 results for: LEA

Clear keywords
Showing: 1981 - 2000 of 12310 results

  1. pKM7

    Plasmid
    #154164
    Purpose
    mCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.
    Depositor
    Kevin Maringer
    Publication
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    mCherry
    Expression
    Insect
    Promoter
    IE1 promoter and hr5 enhancer from Autographa cal…
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154164-plasmid-map-sequence-id-309401

    Enlarge

  5. pKH3-TRIM28-C65A/C68A

    Plasmid
    #92199
    Purpose
    Mammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)
    Depositor
    Huda Zoghbi
    Publication
    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tif1b (TRIM28 Human, Synthetic)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Cysteines 65 and 68 to Alanines.
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92199-plasmid-map-sequence-id-297107

    Enlarge

  16. BPK2797

    Plasmid
    #72251
    Purpose
    Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335Q, and T1337R
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72251-plasmid-map-sequence-id-131872

    Enlarge

  18. VVT1

    Plasmid
    #65779
    Purpose
    The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    SaCas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65779-plasmid-map-sequence-id-112660

    Enlarge

Showing: 1981 - 2000 of 12310 results