We narrowed to 15,989 results for: LEA

1. LCC-ICCG_pET21b

   Plasmid

   #215408

   Purpose

   Expression construct for LCC-ICCG gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   LCC-ICCG
   

   Expression

   Bacterial

   Mutation

   F243I/D238C/S283C/Y127G

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pREDTAI

   Plasmid

   #51627

   Purpose

   E coli Genetic Modification

   Depositor

   Sheng Yang

   Article

   Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

   Insert

   I-SceI (SCEI Budding Yeast)
   

   Use

   Red recombinase and i-scei endonuclease

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDN77

   Plasmid

   #61347

   Purpose

   mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)

   Depositor

   Roland Eils, Barbara Di Ventura

   Article

   Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

   Insert

   mCherry
   

   Tags

   GS linker-AsLov2-biNLS2 and NES IkBa

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pREDTKI

   Plasmid

   #51628

   Purpose

   E coli Genetic Modification

   Depositor

   Sheng Yang

   Article

   Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

   Insert

   I-SceI (SCEI Budding Yeast)
   

   Use

   Red recombinase and i-scei endonuclease

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)

   Plasmid

   #86413

   Purpose

   nuclear split GFP11 reporter with mCherry

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   Nop1pr-GFP11-mCherry-PUS1
   

   Tags

   GFP11 and mCherry

   Expression

   Yeast

   Promoter

   NOP1

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pK7WGF2::hCas9

   Plasmid

   #46965

   Purpose

   Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue

   Depositor

   Sophien Kamoun

   Article

   Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

   Insert

   hCas9
   

   Use

   CRISPR; Plant expression

   Tags

   eGFP

   Promoter

   35S

   Available Since

   Aug. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSJ1679 (pRS315-NOP1pr-GFP11-PUS1)

   Plasmid

   #86414

   Purpose

   nuclear split GFP11 reporter without mCherry

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   NOP1pr-GFP11-PUS1
   

   Tags

   GFP11 and mCherry

   Expression

   Yeast

   Promoter

   NOP1pr

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. T7-RelA

   Plasmid

   #21984

   Depositor

   Warner Greene

   Article

   Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

   Insert

   NFkB p65 subunit (RELA Human)
   

   Tags

   T7 gp10

   Expression

   Mammalian

   Mutation

   For more details for this expression construct,…

   Available Since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pDN41

   Plasmid

   #61344

   Purpose

   mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)

   Depositor

   Roland Eils, Barbara Di Ventura

   Article

   Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

   Insert

   mCherry
   

   Tags

   GS linker-AsLov2-biNLS9 and PKIt NES

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-AsCas12a-Plus-mCherry

    Plasmid

    #191231

    Purpose

    To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity

    Depositor

    Zhili Rong

    Article

    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

    Insert

    AsCas12a-Plus-P2A-mCherry
    

    Use

    CRISPR

    Mutation

    E174R, R951K, R955A

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. NET31 mRFP-N2 (490)

    Plasmid

    #61984

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al PLoS One. 2014 Nov 11;9(11):e111851. doi

    Insert

    NET31 (TMEM209 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCL1161

    Plasmid

    #217964

    Purpose

    For RAPID-release of H3-APEX-eGFP in the Flp-in T-Rex system (ThermoFisher).

    Depositor

    Andrew Bowman

    Article

    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

    Insert

    Histone H3.1 (H3C1 Human)
    

    Tags

    eGFP, APEX

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pREDIA

    Plasmid

    #51625

    Purpose

    E. coli genome modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Expression

    Bacterial

    Promoter

    pRha

    Available Since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRFP-NLS

    Plasmid

    #105296

    Purpose

    To visualize nuclear dynamics in living cells

    Depositor

    Kenneth Yamada

    Article

    Petrie et al Science. 2014 Aug 29;345(6200):1062-5. d

    Insert

    Rac1 (RAC1 Human)
    

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    aa encoding 172-192

    Available Since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. NET33 pEGFP-N2 (597)

    Plasmid

    #61989

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET33 (SCARA5 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDN34

    Plasmid

    #61343

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Tags

    GS linker-AsLov2-biNLS2 and PKIt NES

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCL1324

    Plasmid

    #217965

    Purpose

    Bacterial expression of Importin-5 tagged on its N-terminus with GST. Cleavable with 3C protease.

    Depositor

    Andrew Bowman

    Article

    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

    Insert

    Imp5 (IPO5 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    plac

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. AviTag-SpyTag003-MBP

    Plasmid

    #184227

    Purpose

    Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

    Depositor

    Mark Howarth

    Article

    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

    Insert

    AviTag-SpyTag003-MBP
    

    Tags

    AviTag, His6, SpyTag003, and Thrombin cleavage si…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pREDKI

    Plasmid

    #51626

    Purpose

    E coli Genetic Modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Expression

    Bacterial

    Promoter

    pRha

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. WN10151

    Plasmid

    #80441

    Purpose

    Expresses human codon-optimized dead (832A) LbCpf1

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Insert

    dead (D832A) LbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    D832A

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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