We narrowed to 3,948 results for: SPL

1. SNV-gfp-IRES-MCS

   Plasmid

   #53122

   Purpose

   Retrovirus vector backbone that co-expresses GFP and a gene of interest, driven by the Spleen Necrosis Virus promoter; for use in chicken cells

   Depositor

   Jeanette Hyer

   Article

   Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1

   Type

   Empty backbone

   Use

   Retroviral; For expression in chicken cells

   Tags

   EGFP-IRES

   Expression

   Mutation

   Promoter

   SNV (spleen necrosis virus)

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-bsd-Flag-Trl1

   Plasmid

   #126609

   Purpose

   Expresses N-terminal Flag-tagged yeast Trl1

   Depositor

   Xiaozhong Wang

   Article

   Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

   Insert

   tRNA ligase 1 (TRL1 Budding Yeast)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV/3' Box_(GLuc)_TOP2

   Plasmid

   #68435

   Purpose

   Transient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP2 construct
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSiMPlh_N + pSiMPlh_C

   Plasmid

   #134316

   Purpose

   Maintenance of 2 plasmids with a single antibiotic in E. coli

   Depositor

   Barbara Di Ventura

   Article

   Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

   Insert

   hygR_gp41-1 N-intein + hygR_gp41-1 C-intein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   P254Q in hygR_gp41-1 N-intein

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6_(GLuc)_INT[Spinach2]

   Plasmid

   #68430

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing the "Spinach2" aptamer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. RFP-FKBP-U2AF35

   Plasmid

   #187969

   Purpose

   expresses U2AF35 with N-terminal tags RFP and FKBP

   Depositor

   Markus Wahl

   Article

   Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

   Insert

   U2AF35 (U2af1 Mouse)
   

   Use

   Cmv promoter

   Tags

   mRFP-FKBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. RFP-FKBP-PRPF38A

   Plasmid

   #187968

   Purpose

   expresses PRPF38A with N-terminal tags RFP and FKBP

   Depositor

   Markus Wahl

   Article

   Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

   Insert

   Prp38A (PRPF38A Human, Synthetic)
   

   Use

   Cmv promoter

   Tags

   mRFP-FKBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEBB-II-I-GFP beta isoform

   Plasmid

   #22147

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pEBG-TFII-I alpha

   Plasmid

   #22154

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Tags

   GST and His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pFastBac-His-Flag-Timeless

    Plasmid

    #22892

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

    Insert

    Timeless (TIMELESS Human)
    

    Use

    Tags

    Flag and His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

11. cPE

    Plasmid

    #183549

    Purpose

    Expresses compact Prime Editor in mammalian cells

    Depositor

    Wen Xue

    Article

    Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

    Insert

    SpCas9 nickase (H840A), Reverse Transcriptase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB-puro-V5-Trpt1

    Plasmid

    #126607

    Purpose

    Expresses N-terminal V5-tagged mouse Trpt1

    Depositor

    Xiaozhong Wang

    Article

    Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

    Insert

    tRNA 2' phosphotransferase 1 (Trpt1 Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-IRES-GFP-MyD88-NpLxIS

    Plasmid

    #131347

    Purpose

    Expresses MyD88 with 2 copies of pLxIS motif from STING fused to its N-ternimus

    Depositor

    Jonathan Kagan

    Article

    Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

    Insert

    MyD88-NpLxIS (Myd88 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEBG-TFII-I beta

    Plasmid

    #22155

    Depositor

    Ananda Roy

    Article

    Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Tags

    GST and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pRS416-Aga1p-FAPB2.3.6

    Plasmid

    #158147

    Purpose

    Yeast display reporter for non-display yeast strains (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    Aga1p-FAPB2.3.6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSecEffector

    Plasmid

    #172891

    Purpose

    Contains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901

    Depositor

    Urartu Seker

    Article

    Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

    Inserts

    Bxb1
    TP901
    TEV Display cassette
    ProD promoter between anti aligned Bxb1 sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ProD, nTETO, and pBAD /AraC

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEBB-II-I-EGFP delta isoform

    Plasmid

    #22149

    Depositor

    Ananda Roy

    Article

    Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pU6_(GLuc)_TOP1

    Plasmid

    #68423

    Purpose

    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP1 construct
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. 7iBi89 (rat fibronectin)

    Plasmid

    #14065

    Depositor

    Richard Hynes

    Article

    Huh et al Mol Cell Biol. 1993 Sep . 13(9):5301-14.

    Insert

    Rat Fibronectin 7iBi89 (Fn1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Insert was cut out of pGEM3 with SalI and SacI an…

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pTH-PanCen TALE-mVenusN

    Plasmid

    #191395

    Purpose

    TALE fused to N-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    N-terminal part of split mVenus
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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