We narrowed to 4,065 results for: SPL

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pNTI823

Plasmid

#216632

Purpose

Cal-ID variant with T195/G196 split site, mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID (split site variant)

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAtetO_6xHis-v5.9

Plasmid

#155259

Purpose

Plasmid for the aTc-inducible expression of the v5.9

Depositor

Andrew Ellington

Article

Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

Insert

v5.9

Tags

6xHis

Expression

Bacterial

Promoter

tet PA

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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(302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)

Plasmid

#168192

Purpose

HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRs

Depositor

Christina Woo

Article

Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

Insert

HA-LaG6-(EAAAK)4-OGT(4)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-TRAF6-targeting sgRNA 1

Plasmid

#131345

Purpose

gRNA targeting mouse TRAF6

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

gRNA targeting mouse TRAF6 (Traf6 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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tol2-CAG::ChR2-YFP

Plasmid

#59740

Purpose

Optogenetic excitation of ES cell-derived neurons (and other cells, such as muscle)

Depositor

Ivo Lieberam

Article

Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

Insert

Channelrhodopsin-2

Tags

YFP

Expression

Mammalian

Mutation

H134R

Promoter

CAG

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-pCaMKII-tTAc

Plasmid

#172125

Purpose

Expression of InteinC-tTAC in CaMKII positive cells

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

pCaMKII, tTAc

Use

AAV

Promoter

CaMKII

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG CAG Glucagon-ICAM1-3xHA-pre-mGRASP

Plasmid

#153008

Purpose

Expresses glucagon peptide on mammalian cell surface

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

Glucagon-ICAM1-pre-mGRASP

Tags

3x HA

Expression

Mammalian

Promoter

CAG

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSiMPlc_N + pSiMPlc_C

Plasmid

#134312

Purpose

Maintenance of 2 plasmids with a single antibiotic in E. coli

Depositor

Barbara Di Ventura

Article

Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

Insert

cmR_gp41-1 N-intein + cmR_gp41-1 C-intein

Expression

Bacterial

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-SUN1_888

Plasmid

#125851

Purpose

Expresses SUN1_888 tagged with GFP in mammalian cells

Depositor

Miki Hieda

Article

Nishioka et al Nucleus. 2016 Nov;7(6):572-584. doi: 10.

Insert

SUN1_888 (SUN1 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAW-AlphaLobe

Plasmid

#113255

Purpose

E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

Depositor

Jennifer Doudna

Article

Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

S. pyogenes Cas9 alpha-helical lobe

Tags

10xHis-MBP

Expression

Bacterial

Mutation

residues 56–714; See paper for description

Promoter

T7

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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PEP110E

Plasmid

#97396

Purpose

binary construct of nuclear mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

Nucleus target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pMC051-HisMS2_PLP_pac_LC

Plasmid

#128234

Purpose

Lower Copy expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

Depositor

Paul Freemont

Article

de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

Inserts

Maturation Protein
Coat Protein Dimer

Expression

Bacterial

Promoter

T7

Available Since

Nov. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1 FLIPE-1m

Plasmid

#13544

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207W in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-tag2B-PISP/AIPP1

Plasmid

#47606

Depositor

Emanuel Strehler

Article

Goellner et al Ann N Y Acad Sci. 2003 Apr;986:461-71.

Insert

PISP (PDZD11 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
pMSCV-IRES-GFP-3xFLAG-MyD88-V5

Plasmid

#131351

Purpose

Expresses MyD88 tagged with a 3xFLAG tag at its N-terminus and a V5 tag at its C-terminus

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

MyD88 (Myd88 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pYI001

Plasmid

#170833

Purpose

(GUSPlus::tOCS in pFP100)*

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

GUSPlus::tOCS

Expression

Plant

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-TRAF6-targeting sgRNA 2

Plasmid

#131346

Purpose

gRNA targeting mouse TRAF6

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

gRNA targeting mouse TRAF6 (Traf6 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKIIa-moxBFP-IRES-Flpo

Plasmid

#194977

Purpose

AAV vector to drive expression of moxBFP and Flpo under the control of CamKIIalpha promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

moxBFP, Flpo

Use

AAV

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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PEP112E

Plasmid

#97398

Purpose

binary construct of mitochondria-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

Mitochondria target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Expression scaffold for dCas9+sgRNA

Plasmid

#171799

Purpose

PICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNA

Depositor

Stephen Elledge

Article

Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

Insert

PICASSO: expression scaffold for proper dCas9-fusion + sgRNA expression

Use

CRISPR

Available Since

Aug. 16, 2021

Availability

Academic Institutions and Nonprofits only

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