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pET-28b His6-TEV-SHP2-FL(R4A)

Plasmid

#245192

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 4:2025.07.0

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

R4A

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(E139A)

Plasmid

#245207

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 4:2025.07.0

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

E139A

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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AGG1767 PUb-Bs-TRE-Bn

Plasmid

#242441

Purpose

Ae aegypti PUb promoter expressing barstar, and TRE expressing barnase

Depositor

Luke Alphey

Article

Nevard et al Commun Biol. 2025 Aug 4;8(1):1154. doi:

Inserts

Barstar
DsRED
barnase

Expression

Insect

Promoter

AePUb, Hr5/IE1, and TRE

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pETA_21 tRNA genes

Plasmid

#242431

Purpose

Expresses 21 tRNAs. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site as well as a T7 terminator downstream of each tRNA gene.

Depositor

Sebastian Maerkl

Article

Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:

Insert

21 tRNA genes

Expression

Bacterial

Available Since

Aug. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pBN415

Plasmid

#217920

Purpose

express E. coli E69 (O9a:K30) Wzc with N711Y mutation

Depositor

James Naismith

Article

Yang et al Nat Commun. 2025 Apr 11;16(1):3437. doi:

Insert

E. coli E69 (O9a:K30) Wzc with N711Y mutation

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

N711Y

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pET28_LmFPPS

Plasmid

#186322

Purpose

pET-28a vector (Novagen) (encoding an N-terminal His tag), to transform Escherichia coli BL21 (DE3) cells

Depositor

Sandra Gabelli

Article

Aripirala et al Acta Crystallogr D Biol Crystallogr. 201

Insert

FPPS

Tags

His Tag

Expression

Bacterial

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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delCMV-mCitrine

Plasmid

#214144

Purpose

Volume marker / Control sensor

Depositor

Leif Dehmelt

Article

Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

Insert

mCitrine

Expression

Mammalian

Promoter

delCMV

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2A

Plasmid

#195707

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 124-211 of mouse SYT9

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2B

Plasmid

#195708

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 253-344 of mouse SYT9

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2AB

Plasmid

#195709

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 104-386 of mouse SYT9

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT1 C2A

Plasmid

#195710

Purpose

Bacterial expression plasmid encoding GST-tagged SYT1 C2A domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 96-264 of mouse SYT1

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT1 C2B

Plasmid

#195711

Purpose

Bacterial expression plasmid encoding GST-tagged SYT1 C2B domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 248-421 of mouse SYT1

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT1 C2AB

Plasmid

#195712

Purpose

Bacterial expression plasmid encoding GST-tagged SYT1 C2AB domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 96-421 of mouse SYT1

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET (SUMO)-Syt9

Plasmid

#195713

Purpose

Bacterial expression plasmid encoding SUMO-tagged full-length Syt9

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Rat)

Tags

GST-SUMO

Expression

Bacterial

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-GFP RanSen

Plasmid

#184697

Purpose

Lentiviral transfer plasmid for expression of GFP RanSen

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP RanSen

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-ArgSen (-)NLS

Plasmid

#184689

Purpose

Lentiviral transfer plasmid for expression of ArgSen (-)NLS

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

ArgSen (-)NLS

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-ArgSen

Plasmid

#184691

Purpose

Lentiviral transfer plasmid for expression of ArgSen

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

ArgSen

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-ArgSen

Plasmid

#184700

Purpose

Lentiviral transfer plasmid for expression of ArgSen

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

ArgSen

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbR-His-ELP(MV7E2)3-ddFLN4-SpyCatcher

Plasmid

#157674

Purpose

Expression of SpyCatcher with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

Depositor

Michael Nash

Article

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

SpyCatcher with ddFLN4, ELP, Histag and ybbR tag

Tags

ddFLN4, ELP, Histag and ybbR tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pQE80L-His-SpyTag-XMod(XL2V)-Doc

Plasmid

#157676

Purpose

Expression of XMod-Doc mutant XL2V (All LEUs were mutated to VAL in XMod region) with His6x tag and SpyTag.

Depositor

Michael Nash

Article

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

XMod-Doc mutant XL2V with Histag and SpyTag

Tags

Histag and SpyTag

Expression

Bacterial

Promoter

T5

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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