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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,215 results for: N-EGFP

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Showing: 2001 - 2020 of 3215 results

  5. pDEST-CMV-polysite

    Plasmid
    #122843
    Purpose
    Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Type
    Empty backbone
    Use
    Gateway destination
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    122843-plasmid-map-sequence-id-244374

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  9. pBA900

    Plasmid
    #122237
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs2 at the 3' end of the constant region
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    122237-plasmid-map-sequence-id-292010

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  10. pBA950

    Plasmid
    #122239
    Purpose
    CROP-seq vector optimized for sgRNA expression and CRISPRi activity. This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    BFP selectable marker, plus a modified mU6 promoter and sgRNA constant region for optimized CRISPRi activity
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122239-plasmid-map-sequence-id-292012

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  16. dRED

    Plasmid
    #139836
    Purpose
    Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells
    Depositor
    Karim Mekhail
    Article
    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:
    Insert
    dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))
    Expression
    Mammalian
    Mutation
    RNase H1 protein contains D210N mutation. dCas9 …
    Promoter
    tet-inducible CMV
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139836-plasmid-map-sequence-id-308228

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  17. RED

    Plasmid
    #139835
    Purpose
    Expresses RNase H1-EGFP- dCas9 fusion protein in human cells
    Depositor
    Karim Mekhail
    Article
    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:
    Insert
    RNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))
    Expression
    Mammalian
    Mutation
    dCas9 protein contains two mutations: D10A and H8…
    Promoter
    tet-inducible CMV
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139835-plasmid-map-sequence-id-310615

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  18. pUt-mPF-BACH1 Cas9-Resistant_AmpR

    Plasmid
    #199221
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Positive-Feedback circuit (mPF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    rtTA-Advanced::P2A::EGFP::BACH1 (BACH1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    silient mutation at nucleic acid 177 C changed to…
    Promoter
    tight TRE promoter
    Available Since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199221-plasmid-map-sequence-id-396541

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Showing: 2001 - 2020 of 3215 results