182,051 results
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Plasmid#107175Purposeexpresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vectorDepositorInsertTurboID (BirA mutant)
UseLentiviralMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Available sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
4XCLEAR-luciferase reporter
Plasmid#66800PurposeReporter for activity of the CLEAR networkDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert4XCLEAR
UseLuciferasePromoterFour CLEAR elements (GTCACGTGAC) in tandem derive…Available sinceNov. 5, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TFEB promoter-luciferase reporter
Plasmid#66801PurposeReporter for activity of TFEB gene promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTFEB promoter (Tfeb Mouse)
UseLuciferaseAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSB700
Plasmid#64046PurposeLentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent proteinDepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyExpressionMammalianPromoterU6, CAGGSAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRCT
Plasmid#60621PurposePlasmid encoding iCas9, tracrRNA and crRNAsDepositorPublicationInsertsiCas9
tracrRNA
UseCRISPRTagsFLAG and SV40 NLSExpressionYeastMutationchanged Aspartate 147 to Tyrosine, Proline 411 to…PromoterRPR1p and TEF1pAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PH-Btk-GFP
Plasmid#51463PurposeIs a biosensor for PIP3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBTK (aa 1–177) (BTK Human)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC119-gRNA
Plasmid#52255PurposeCan use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.DepositorInsertguide RNA targeting AtPDS3 (PDS3 Mustard Weed)
UseCRISPR; Plant expressionPromoterArabidopsis U6 polymerase III promoterAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato
Plasmid#51083PurposeCre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophoreDepositorHas serviceAAV RetrogradeInsertGCaMP6f
UseAAVTagsP2A-nls-dTomatoExpressionMammalianPromoterhEF1aAvailable sinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
C321.ΔA
Bacterial strain#48998PurposeRecoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted)DepositorBacterial resistanceAmpicillinSpeciesEscherichia coliAvailable sinceOct. 24, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEN-luxCDABE
Plasmid#44918DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsigma 70 promoter
UseLuciferaseTagslux transcriptional fusionPromoterem7Available sinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1 Flag hHMGB1
Plasmid#31609DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHMGB1 (HMGB1 Human)
TagsFlagExpressionMammalianPromoterCMVAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
wtTDP43tdTOMATOHA
Plasmid#28205DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTar DNA binding protein (TARDBP Human)
TagsHA tag and tdtomatoExpressionMammalianAvailable sinceJune 1, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BirA in pET28a (w400-2)
Plasmid#26624DepositorInsertBirA
TagsHisExpressionBacterialAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
2XMyc-LRRK2-WT
Plasmid#25361DepositorPublicationAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
N-Myc-53BP1 WT pLPC-Puro
Plasmid#19836DepositorAvailable sinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJL43.1 - Pglh-2::MosTase::glh-2utr
Plasmid#19332DepositorPublicationInsertPglh-2::Mos1 transposase
ExpressionWormAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCyPet-His
Plasmid#14030DepositorInsertCyPet
Tags6xHisExpressionBacterialAvailable sinceFeb. 14, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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413 pSG5L HA RB
Plasmid#10720DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRB (RB1 Human)
TagsHAExpressionMammalianAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEG BacMam
Plasmid#160451Purposevector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneExpressionMammalianPromoterCMVAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by