169,697 results

1. JAK2 (V617F)-pcw107-V5

   Plasmid

   #64610

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   JAK2 (JAK2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   V617F

   Promoter

   PGK

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV.rTH.PI.Cre.SV40 (AAV Retrograde)

   Viral Prep

   #107788-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from AAV.rTH.PI.Cre.SV40 (#107788). In addition to the viral particles, you will also receive purified AAV.rTH.PI.Cre.SV40 plasmid DNA. rTH promoter driving Cre expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   rTH

   Tags

   None

   Available since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-Ef1a-fDIO-JRGECO1a (AAV1)

   Viral Prep

   #128317-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-Ef1a-fDIO-JRGECO1a (#128317). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-JRGECO1a plasmid DNA. EF1a-driven, Flp-dependent expression of the biosensor jRGECO1a. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Patricia Jensen

   Promoter

   EF1a

   Tags

   None

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

4. pSpCas9_T2A_mCherry sg1-sg2

   Plasmid

   #192479

   Purpose

   All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Use

   Tags

   T2A mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-SFFV-GFP1-10

   Plasmid

   #80409

   Purpose

   Expresses GFP1-10 in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   GFP1-10
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.GFAP.Cre.WPRE.hGH

   Plasmid

   #105550

   Purpose

   AAV expression of Cre from GFAP promoter

   Depositor

   James M. Wilson

   Has Service

   AAV PHP.eB and AAV5

   Insert

   Cre
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. mito-PAGFP

   Plasmid

   #23348

   Purpose

   Expresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells.

   Depositor

   Richard Youle

   Article

   Karbowski et al J Cell Biol. 2004 Feb 16. 164(4):493-9.

   Insert

   PAGFP
   

   Use

   Tags

   mito

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7 RNA POL

   Plasmid

   #138352

   Purpose

   Expressing T7 RNA Polymerase in mammalian cells

   Depositor

   Baisong Lu

   Insert

   T7 RNA POL
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTNS2

   Plasmid

   #64968

   Purpose

   Tn7 transposase expression

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Insert

   tnsABCD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. mCherry-GAL9

    Plasmid

    #166689

    Purpose

    For creating a stable mCherry-GAL9 cell line

    Depositor

    Alan Sabirsh

    Article

    Munson et al Commun Biol. 2021 Feb 16;4(1):211. doi:

    Insert

    LGALS9 (LGALS9 Human)
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. psPAX2-D64V

    Plasmid

    #63586

    Purpose

    psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.

    Depositor

    David Rawlings, Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Packaging plasmid for integrase deficient lentiviral vectors
    

    Use

    Integrase deficient lentiviral packaging vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. P336-FBP_11

    Plasmid

    #139702

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing Luz

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX458-AAVS1

    Plasmid

    #113194

    Purpose

    Encodes gRNA for human AAVS1

    Depositor

    Adam Karpf

    Insert

    AAVS1 (PPP1R12C, AAVS1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV9)

    Viral Prep

    #105545-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    HI

    Tags

    EGFP

    Available since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pT3-EF1aH N90-beta-catenin

    Plasmid

    #86499

    Purpose

    Mammalian expression of Myc tagged N90 beta-catenin

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 1-90; constitutively acti…

    Promoter

    EF1a

    Available since

    Feb. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-C2-NLRP3

    Plasmid

    #73955

    Purpose

    Express EGFP-tagged human NLRP3 in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Khare et al Immunity. 2012 Mar 23;36(3):464-76. doi:

    Insert

    NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV iE

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. mRFP-Lact-C2

    Plasmid

    #74061

    Purpose

    Fluorescent biosensor for phosphatidylserine.

    Depositor

    Sergio Grinstein

    Article

    Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

    Insert

    Lactadherin-C2 (MFGE8 Bovine)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. YAP/TAZ-TRE-mStrawberry reporter

    Plasmid

    #158682

    Purpose

    This plasmid is a YAP/TAZ pathway reporter. It has a YAP/TAZ-responsive synthetic promoter driving the expression of mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. SBE4-Luc

    Plasmid

    #16495

    Purpose

    Luciferase reporter containing four copies of the Smad binding site

    Depositor

    Bert Vogelstein

    Article

    Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.

    Insert

    Smad binding element
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. dCas9-VPR_P2A_mCherry

    Plasmid

    #154193

    Purpose

    Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    sp-dCas9-VPR_P2A_mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1as

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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