Addgene

We narrowed to 13,155 results for: Mpl;

Showing: 4081 - 4120 of 13155 results

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pLD-hygro-EnVM

Plasmid

#24590

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VM

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-CTE-Luc

Plasmid

#60881

Purpose

Reporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTE

Depositor

Vinay Nandicoori

Article

Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371

Insert

Luc-CTE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pXWJ109As-gfp

Plasmid

#123143

Purpose

An arsenic sensor with optimized intracellular receptor density. pSB3K3 carrying J109-30arsR-t-ParsR-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-30arsR-t-ParsR-30gfp-t

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J109, ParsR

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSR47

Plasmid

#69258

Purpose

pmyo-3::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Tags

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

myo-3

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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ptcALPS gag-gfp Blasti

Plasmid

#115806

Purpose

Express gag-gfp fusion driven by TK promoter

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gag-gfp

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TK

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P01

Plasmid

#120012

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxBFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxBFP

Use

Pcr template

Tags

moxBFP

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCk1

Plasmid

#136695

Purpose

Acceptor plasmid for odd level position 1, lacZ blue-white screening. Part of the OpenPlant toolkit.

Depositor

Nicola Patron

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-MSL3L1

Plasmid

#29761

Depositor

Joan Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

MSL3L1 (MSL3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2013

Availability

Academic Institutions and Nonprofits only
Sa-SchCas9

Plasmid

#192137

Purpose

Expresses Sa-SchCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

Insert

Sa-SchCas9

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLD-puro-PGKnVA

Plasmid

#24589

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pCFJ1359 - Intron GG2 - smu-1

Plasmid

#159805

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG2 - smu-1

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLBC0010

Plasmid

#172774

Purpose

Level 0 module containing NT1_CTPSSU

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Gene sequence for Rubisco small subunit (SSU) chloroplast transit peptide (CTP) of Arabidopsis thaliana

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0011

Plasmid

#172775

Purpose

Level 0 module containing NT2_CTPSSU

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Gene sequence for Rubisco small subunit (SSU) chloroplast transit peptide (CTP) of Arabidopsis thaliana

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0060

Plasmid

#172783

Purpose

Level 0 module containing NT2_cYFP3XHA

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS C-terminal eYFP (175-238) 3XHA tag

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0061

Plasmid

#172784

Purpose

Level 0 module containing CT_cYFP3XHA

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS C-terminal eYFP (175-238) 3XHA tag

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDF0385 pcmv-hudiscas7-11-r1045-gggs-r1122

Plasmid

#186982

Purpose

mammalian expression of cas7-11-r1045-gggs-r1122

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-r1045-gggs-r1122

Use

Tags

Expression

Mammalian

Mutation

r1045-gggs-r1122 deletion

Promoter

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2215 - Intron GG3 - 900 bp

Plasmid

#159882

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG3 - 900 bp

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P32

Plasmid

#120043

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with S-Tag

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

S-Tag

Use

Pcr template

Tags

S-Tag

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJZC593

Plasmid

#62319

Purpose

sgRNA with MS2-PP7 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + MS2-PP7 RNA binding module

Use

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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A3G-Cas9n-UGI

Plasmid

#119139

Purpose

Base editor made from APOBEC3G

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3G-Cas9 nickase-UGI

Use

CRISPR

Tags

Expression

Mutation

None

Promoter

CMV

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCRII-HSV2.3 TCR beta chain

Plasmid

#74077

Purpose

gBT-I TCR beta chain cDNA in pCRII

Depositor

Francis R. Carbone

Article

Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

Insert

TCR beta cDNA clone HSV2.3 (Tcrb Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pBacPAK8-His-VHL-Flag

Plasmid

#29497

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Von Hippel-Lindau (VHL Human)

Use

Tags

Flag and His

Expression

Insect

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag-Tpr-Si

Plasmid

#60918

Purpose

Expresses Flag tagged nucleoporin Tpr resistant to siRNA GCACAACAGGATAAGGTTA

Depositor

Vinay Nandicoori

Article

Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371

Insert

TPR (TPR Human)

Use

Tags

Expression

Mammalian

Mutation

Silent point mutations in siRNA target region; E1…

Promoter

CMV

Available since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z12

Plasmid

#120055

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet

Use

Pcr template

Tags

mScarlet

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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GST-rhodopsin-CTS

Plasmid

#86071

Purpose

A bacterial expression plasmid encoding rhodopsin ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Rhodopsin (RHO Bovine)

Use

Tags

GST

Expression

Bacterial

Mutation

contains the region from 309-348 of Rhodopsin

Promoter

tac

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z25

Plasmid

#120068

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with YFP-LID

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

YFP-LID

Use

Pcr template

Tags

YFP-LID

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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BacPAK Flag-NFRKB

Plasmid

#19791

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

NFRKB (NFRKB Human)

Use

Tags

Flag and His

Expression

Insect

Mutation

Promoter

Available since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
PX544 SpCas9(del1098-1368)

Plasmid

#58889

Purpose

Truncated form of SpCas9 without C' domain

Depositor

Feng Zhang

Article

Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

del amino acids 1098-1368

Promoter

Available since

Sept. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P08

Plasmid

#120019

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mClover3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mClover3

Use

Pcr template

Tags

mClover3

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmGW182

Plasmid

#22406

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

gawky, variant A (gw Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Expression

Mutation

Promoter

Available since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-P19

Plasmid

#124789

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry-mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry-mNeonGreen

Use

Pcr template

Tags

mCherry-mNeonGreen

Expression

Mutation

Promoter

None

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE5

Plasmid

#79461

Purpose

sgRNA shuttle vector; module 1

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH579

Plasmid

#172163

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD JM10.1); AraC

Use

Tags

Expression

Bacterial

Mutation

Sumo(S6L) PylB(N61S, E84K, E122K, E175K, E178K, G…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac-His-Flag-Tipin

Plasmid

#22890

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Tipin (TIPIN Human)

Use

Tags

Flag- and His

Expression

Insect

Mutation

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pMaCTag-Z29

Plasmid

#120072

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Strep-II

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Strep-II

Use

Pcr template

Tags

Strep-II

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-Z03

Plasmid

#120046

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxCerulean3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxCerulean3

Use

Pcr template

Tags

moxCerulean3

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS2374-Tier1-PhCMV-Gal4_VP16

Plasmid

#169582

Purpose

Tier-1 vector encoding PhCMV-driven Gal4-VP16 transactivator (PhCMV-Gal4-VP16-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

GAL4-transactivator

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Pediculus humanus corporis PINK1 (143 - 575)

Plasmid

#110751

Purpose

Bacterial expression of PhPINK1 (crystallography)

Depositor

David Komander

Article

Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

Insert

Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)

Use

Tags

N-His6-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBMN mCherry-NBR1(ΔUBD)

Plasmid

#119691

Purpose

For mammalian expression of NBR1 without its UBD

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

NBR1 (NBR1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

UBD deletion (1-906)

Promoter

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBP_hBrg1 (J1 antibody)

Plasmid

#26109

Depositor

Jerry Crabtree

Article

Wang et al EMBO J. 1996 Oct 1. 15(19):5370-82.

Insert

Brg1 (SMARCA4 Human)

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 4081 - 4120 of 13155 results

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