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Plasmid#145133PurposeExpresses CCH domain from p130Cas with an mCherry tagDepositorInsertp130Cas CCH domain (BCAR1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti Cas9/SB100
Plasmid#155294PurposeLentiviral expression of Cas9 and SB100DepositorInsertSB100X-P2A-spCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huLb5Cas12a
Plasmid#174684PurposeExpresses 6xHis-MBP tagged Lb5Cas12aDepositorArticleInserthuLb5Cas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialMutationPromoterT7Available sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-LmoCas6-p300
Plasmid#126486PurposeEncodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoterDepositorInsertLmoCas6-p300
UseCRISPRTagsFlag and Flag, NLSExpressionMammalianMutationPromoterCMV and U6Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Cas12c2-noncoding
Plasmid#120877PurposeExpresses concatenated noncoding sequences surrounding the CRISPR-Cas12c2 system in the native genomic loci; expresses the tracrRNA required for activity.DepositorInsertNoncoding sequences for CRISPR-Cas12c2
UseCRISPRTagsExpressionBacterialMutationPromoterlacAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR-act5cB-LexAQF
Plasmid#61310PurposeExpresses chimeric transactivator LexA_DBD:QF2_AD in DrosophilaDepositorInsertLexA_DBD::QF2_AD
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3s-eCas9-1.1
Plasmid#104172PurposeeCas9-1.1 mammalian expressionDepositorInserteCas9-1.1
UseCRISPRTagsHAExpressionMammalianMutationK848A, K1003A, R1060APromoterCMVAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2
Plasmid#107727PurposeKnock out of EB1 in human cells by CRISPR/Cas9DepositorInsertMAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFREE2-xCas9-sgRNA
Plasmid#179579PurposeExpresses xCas9 and gRNA that targets ColE1 plasmids for curing.DepositorInsertxCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEJS1026-pMCSG7-HpaCas9
Plasmid#121540PurposeExpresses a 6X-His tagged type II-C Cas9 from H. parainfluenzae in bacterial cellsDepositorInsertHpaCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
RCAS (A) Six1 (CT#664)
Plasmid#13876DepositorInsertSix1 (Six1 Mouse)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
ciCas9(L22)_pcDNA5
Plasmid#100551PurposeExpresses ciCas9(L22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.DepositorInsertciCas9(L22)
UseCRISPR; Frt, flp-inTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huHkCas12a
Plasmid#174683PurposeExpresses 6xHis-MBP tagged HkCas12aDepositorArticleInserthuHkCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHAExpressionBacterialMutationPromoterT7Available sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEJS1027-pMCSG7-SmuCas9
Plasmid#121541PurposeExpresses a 6X-His tagged type II-C Cas9 from S. muelleri in bacterial cellsDepositorInsertSmuCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-SpCas9
Plasmid#130968Purpose3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-SpCas9
UseCRISPRTags3x HA, EGFP, and NLSExpressionMammalianMutationPromoterCbhAvailable sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-T-deGFP
Plasmid#184840PurposeExpression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.DepositorInsertsingle-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and lac promoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
A3D-Cas9n-UGI
Plasmid#119137PurposeBase editor made from APOBEC3DDepositorInsertAPOBEC3D-Cas9 nickase-UGI
UseCRISPRTagsExpressionMutationNonePromoterCMVAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC92-pmax-dCas9VP96
Plasmid#48224PurposedCas9VP96 on pmax expression vectorDepositorInsertdCas9(D10A;H840A) fusion with VP96 activation domain
UseCRISPRTagsHA Tag and VP96ExpressionMammalianMutationD10A;H840APromoterCAGGSAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
zCREST3:Cas9green; T3_gRNA
Plasmid#199335PurposepDEL161; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type III sgRNADepositorInsertszCREST3
Cas9
nrg1 type III sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-DR-GC-swap-cr in pBCSK+
Plasmid#126643PurposeCloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cellsDepositorInsertLbCas12a Full Length Direct Repeat GC Swap crRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only