We narrowed to 34,868 results for: CaS;

1. SP6-hCas9-Ce-mRNA

   Plasmid

   #47911

   Purpose

   In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.

   Depositor

   Paul Sternberg

   Article

   Chiu et al Genetics. 2013 Aug 26.

   Insert

   hCas9 with C. elegans 5' and 3' UTRs
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   SP6

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX165-Cas9-HF1

   Plasmid

   #140565

   Purpose

   Expresses Cas9-HF1 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mutation

   N497A R661A Q695A Q926A

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiCas9-sgCh2-2-Blast

   Plasmid

   #199645

   Purpose

   Expresses Cas9 and sgRNA control guide targeting intergenic region

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC1806_pmax-FKBP-dCasRx

   Plasmid

   #118639

   Purpose

   Transient transfection; Expresses FKBP-dCasRx; CAGGS promoter

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   FKBP-dCasRx
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-AsCas12f-v4.1

   Plasmid

   #204636

   Purpose

   Expression of AsCas12f v4.1 in mammalian cells

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   AsCas12f-v4.1
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   D169K, N199K, N328G, D364R

   Promoter

   CMV

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. RCAS(A) mPea3 FL (CT#1119)

   Plasmid

   #13908

   Depositor

   Cliff Tabin

   Article

   Brent et al Development. 2004 Aug . 131(16):3885-96.

   Insert

   Pea3 (Etv4 Mouse)
   

   Use

   Retroviral; Avian expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

7. RCAS GH6 (CC#152)

   Plasmid

   #15137

   Depositor

   Connie Cepko

   Article

   Schulte et al Development. 2000 Dec . 127(23):5033-45.

   Insert

   GH6 (HMX1 Chicken)
   

   Use

   Retroviral; Avian expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 15, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pENTR2B-dCas9-KRAB

   Plasmid

   #115547

   Purpose

   Entry cassette for dCas9-KRAB

   Depositor

   Rene Maehr

   Article

   Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

   Insert

   dCas9-KRAB
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. LIC1B_Caspase-LOVN7C-7 C450A

   Plasmid

   #104628

   Purpose

   bacterial expression of light-activated caspase-3 with N7C-7 linker variation on C450A mutation

   Depositor

   Jim Wells

   Article

   Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

   Insert

   Caspase-3 (CASP3 Human)
   

   Use

   Tags

   His and LOV2

   Expression

   Bacterial

   Mutation

   C450A (dark-locked and light-insensitive mutation)

   Promoter

   T7

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMS8: pTns(AvCAST)

    Plasmid

    #168141

    Purpose

    Inducible expression of AvCAST TnsA, TnsB, TnsC, TniQ and TnsD proteins.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
    AvTnsD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas9-beta-mmr

    Plasmid

    #169241

    Purpose

    Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Inserts

    cas9
    beta
    mutL-E36K
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed glutamic acid 36 to lysine

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459-HypaCas9-ECT2_sgRNA

    Plasmid

    #183873

    Purpose

    pX459V2.0-HypaCas9 plasmid with ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ECT2 sgRNA spacer (ECT2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459-HypaCas9-AAVS1_sgRNA

    Plasmid

    #183891

    Purpose

    pX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 sgRNA spacer (AAVS1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-PxCas12a-2AeGFP

    Plasmid

    #123637

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    PxCas12a
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX551-miniCMV-CjCas9

    Plasmid

    #107033

    Purpose

    AAV plasmid expressing CjCas9 under control of miniCMV promoter

    Depositor

    Alex Hewitt

    Insert

    CjCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpyCas9 PE2*-3HA

    Plasmid

    #169854

    Purpose

    Mammalian Expression, SpyCas9 PE2* prime editor with 3HA-tag

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SpCas9-H840A-star-M-MLV-3HA
    

    Use

    Tags

    3XHA tag, bpSV40 NLS and SV40 NLS, and cmyc-NLS a…

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ141-ZmUbi-RZ-Cas12j2

    Plasmid

    #173926

    Purpose

    Gateway entry clone (attL5 & attL2) for Cas12j2 crRNA expression under ZmUbi promoter with ribozyme processing

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330_ACTB-i4 sgRNA / hSpCas9

    Plasmid

    #172828

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAS9-mCherry-ACTG1

    Plasmid

    #66943

    Purpose

    CRISPaint target selector ACTG1

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA ACTG1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX551-miniCMV-SaCas9

    Plasmid

    #107032

    Purpose

    AAV plasmid expressing SaCas9 under control of miniCMV promoter

    Depositor

    Alex Hewitt

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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