We narrowed to 18,992 results for: Met

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SK-YFP-SPNM-B

Plasmid

#48661

Purpose

Bacterial SP and NM repression YFP reporter: protospacer B

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

SP/NM prototspacer B/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal Katushka

Plasmid

#104029

Purpose

Plasmid encoding 2ndVal Katushka

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal Katushka

Use

Low copy

Expression

Bacterial

Mutation

Katushka with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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DS-ST1casN-

Plasmid

#48659

Purpose

Bacterial nuclease-null ST1 Cas9 expression

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9, nuclease-null
ST1 tracrRNA

Use

CRISPR

Mutation

D to A, DH to AA, N to A

Promoter

proC

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pAP974-1

Plasmid

#99497

Purpose

TEV::linker::tagRFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::linker::tagRFP::3XFlag

Expression

Bacterial

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pEB2-TurboRFP

Plasmid

#103996

Purpose

Plasmid encoding TurboRFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

TurboRFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (R32G, T68A, L79F, …

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pXN-FBLWLF-LexA

Plasmid

#26266

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

LexA

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pXY1-spc

Plasmid

#90488

Purpose

Plasmid with ptb promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Type

Empty backbone

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pXY1

Plasmid

#90487

Purpose

Plasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Type

Empty backbone

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pNCS-CyRFP1

Plasmid

#84546

Purpose

Bacterial expression of CyRFP1

Depositor

Michael Lin

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

CyRFP1

Tags

His

Expression

Bacterial

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pAL-pyk_NT

Plasmid

#74072

Purpose

pAL374 plasmid carrying the pyk (NT) sgRNA targeting the non-template strand of pyk, SpecR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA pyk (NT)

Use

CRISPR

Expression

Bacterial

Promoter

ptac

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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hgRNA-A21_pLKO-Hyg

Plasmid

#100559

Purpose

hgRNA-A21 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

hgRNA-A21

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-coVenNB

Plasmid

#104019

Purpose

Plasmid encoding coVenNB

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

coVenNB

Use

Low copy

Expression

Bacterial

Mutation

Same protein sequence as Venus NB (codon optimize…

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-mEGFP

Plasmid

#103979

Purpose

Plasmid encoding mEGFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mEGFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-Vx3(A7)

Plasmid

#35526

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

Vx3(A7)

Tags

His6

Expression

Bacterial

Mutation

Changed G2 to P

Promoter

T7

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

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DS-TDcasN-

Plasmid

#48660

Purpose

Bacterial nuclease-null TD Cas9 expression

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9, nuclease-null
TD tracrRNA

Use

CRISPR

Mutation

D to A, DH to AA, N to A

Promoter

proC and tracrRNA promoter

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-Map7emtb

Plasmid

#46078

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7emtb

Tags

GFP

Expression

Mammalian

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-act5cB-LexAQF

Plasmid

#61310

Purpose

Expresses chimeric transactivator LexA_DBD:QF2_AD in Drosophila

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexA_DBD::QF2_AD

Expression

Insect

Available Since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pEB1-2ndVal mGFPmut2

Plasmid

#104033

Purpose

Plasmid encoding 2ndVal mGFPmut2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mGFPmut2

Use

Low copy

Expression

Bacterial

Mutation

mGFPmut2 with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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PM-SP!TB

Plasmid

#48650

Purpose

Bacterial SP crRNA expression: targets SP to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pEB1-comTurquoise2

Plasmid

#104018

Purpose

Plasmid encoding comTurquoise2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

comTurquoise2

Use

Low copy

Expression

Bacterial

Mutation

Same protein sequence as mTurquoise2 (codon optim…

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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