We narrowed to 2,180 results for: PAM

Showing: 2041 - 2060 of 2180 results

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GFP-FKBP-LBR

Plasmid

#222427

Purpose

To express LBR with N-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

LBR (LBR Human)

Tags

GFP-FKBP

Expression

Mammalian

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pJT309 RapInducible1-ActivatorCloneSite-Citrine

Plasmid

#187321

Purpose

Rapamycin inducible genetic circuit with cloning site for activator domains

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Type

Empty backbone

Expression

Mammalian

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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DRD4-DuET

Plasmid

#213230

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

DRD4 (DRD4 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMX1463

Plasmid

#208805

Purpose

Expresses plasma-membrane-bound FRB-SBFP2 for rapid relocalization of cytoplasmic proteins tagged with FKBP upon addition of rapamycin

Depositor

Linda Wordeman

Article

Wordeman et al Mol Biol Cell. 2016 Apr 15;27(8):1300-9.

Insert

FRB (CRP Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pvLEA22mer_shuffle_3_mCh-2xFKBP (pBS1119)

Plasmid

#185314

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_shuffle_3

Expression

Mammalian

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pST_Q19228_CAEEL_93-127 (pBS0735)

Plasmid

#185231

Purpose

For the mammalian expression of the C. elegans protein Q19228_CAEEL_93-127. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q19228_CAEEL_93-127

Expression

Mammalian

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOA4_ANDDA_21-367 (pBS0742)

Plasmid

#185234

Purpose

For the mammalian expression of the Chinese giant salamander protein APOA4_ANDDA_21-367. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOA4_ANDDA_21-367

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_Q7JLY3_CAEEL_48-124 (pBS0738)

Plasmid

#185232

Purpose

For the mammalian expression of the C. elegans protein Q7JLY3_CAEEL_48-124. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q7JLY3_CAEEL_48-124

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_Q19228_CAEEL_51-127_noLink (pBS0733)

Plasmid

#185229

Purpose

For the mammalian expression of the C. elegans protein Q19228_CAEEL_51-127_noLink. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q19228_CAEEL_51-127_noLink

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_H2FLK5_CAEEL_199-435 (pBS0696)

Plasmid

#185215

Purpose

For the mammalian expression of the C. elegans protein H2FLK5_CAEEL_199-435. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

H2FLK5_CAEEL_199-435

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_H2FLK5_CAEEL_542-800 (pBS0695)

Plasmid

#185214

Purpose

For the mammalian expression of the C. elegans protein H2FLK5_CAEEL_542-800. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

H2FLK5_CAEEL_542-800

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DrHD_b03-dom3_opt2 (pBS0693)

Plasmid

#185213

Purpose

For the mammalian expression of the Deinococcus radiodurans protein DrHD_b03-dom3_opt2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DrHD_b03-dom3_opt2

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DrHD_b03-dom3_opt1 (pBS0692)

Plasmid

#185212

Purpose

For the mammalian expression of the Deinococcus radiodurans protein DrHD_b03-dom3_opt1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DrHD_b03-dom3_opt1

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_Q96Q06-2_DrHD_b03_dom3 (pBS0687)

Plasmid

#185211

Purpose

For the mammalian expression of the Deinococcus radiodurans protein Q96Q06-2_DrHD_b03_dom3. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q96Q06-2_DrHD_b03_dom3

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_Q96Q06-2_DrHD_b03_dom1 (pBS0676)

Plasmid

#185204

Purpose

For the mammalian expression of the Deinococcus radiodurans protein Q96Q06-2_DrHD_b03_dom1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q96Q06-2_DrHD_b03_dom1

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_CAHS1_RAMVA_108-197_noLink (pBS0654)

Plasmid

#185198

Purpose

For the mammalian expression of the tardigrade protein CAHS1_RAMVA_108-197_noLink. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS1_RAMVA_108-197_noLink

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_CAHS1_PARRC_101-189_noLink (pBS0653)

Plasmid

#185197

Purpose

For the mammalian expression of the tardigrade protein CAHS1_PARRC_101-189_noLink. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS1_PARRC_101-189_noLink

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_CAHS1_RAMVA_108-197 (pBS0652)

Plasmid

#185196

Purpose

For the mammalian expression of the tardigrade protein CAHS1_RAMVA_108-197. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS1_RAMVA_108-197

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_CAHS1_PARRC_101-189 (pBS0651)

Plasmid

#185195

Purpose

For the mammalian expression of the tardigrade protein CAHS1_PARRC_101-189. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS1_PARRC_101-189

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_E4_80-255 (pBS0647)

Plasmid

#185192

Purpose

For the mammalian expression of the human protein APOE_HUMAN_E4_80-255. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_E4_80-255

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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