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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 2,117 results for: Pam

Showing: 2041 - 2060 of 2117 results

  3. DRD2-DuET

    Plasmid
    #213228
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    DRD2 (DRD2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213228-plasmid-map-sequence-id-430789

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  4. DRD1-DuET

    Plasmid
    #213227
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    DRD1 (DRD1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213227-plasmid-map-sequence-id-431800

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  5. pAcGP67A-TfR

    Plasmid
    #12130
    Depositor
    Pamela Bjorkman
    Article
    Lebron et al Cell. 1998 Apr 3. 93(1):111-23.
    Insert
    transferrin receptor (TFRC Human)
    Use
    Tags
    6X-His tag, Factor Xa cleavage site, and leader p…
    Expression
    Insect
    Mutation
    The portion of the human TfR gene encoding residu…
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only

  7. pcDNA3.1.D2-NP

    Plasmid
    #134366
    Purpose
    Nanoluc complementation assay. Expression of dopamine receptor D2 fused at C terminus with Natural peptide (NP) of NanoLuc. Addition of the signal sequence and Flag epitope at N terminus of D2.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    D2-NP (DRD2 Human)
    Use
    Tags
    Flag, natural peptide of nanoluciferase, and sign…
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134366-plasmid-map-sequence-id-306391

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  9. CD109-bio-His

    Plasmid
    #51862
    Purpose
    Expresses full-length CD109 antigen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CD109 (CD109 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51862-plasmid-map-sequence-id-79754

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  10. LLPS1-iLID::EGFP::FTH1 (pBS1042)

    Plasmid
    #185289
    Purpose
    For the mammalian expression of the synthetic protein iLID::eGFP::FTH1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    iLID::eGFP::FTH1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185289-plasmid-map-sequence-id-363896

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  11. DRD5-DuET

    Plasmid
    #213231
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    DRD5 (DRD5 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213231-plasmid-map-sequence-id-430791

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  15. FKBP-DCRD-YFP

    Plasmid
    #87450
    Purpose
    rapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channels
    Depositor
    Xiaodong Liu
    Article
    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1
    Insert
    DCRD (distal C-terminal regulatory domain) (CACNA1F Human)
    Use
    Tags
    6xHis-T7, FKBP, and YFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87450-plasmid-map-sequence-id-174914

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  17. CFP-PCRD-FRB

    Plasmid
    #87449
    Purpose
    rapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channels
    Depositor
    Xiaodong Liu
    Article
    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1
    Insert
    PCRD (proximal C-terminal regulatory domain) (CACNA1F Human)
    Use
    Tags
    6xHis-T7, CFP, and FRB
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87449-plasmid-map-sequence-id-174912

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  18. L2088-Mut-GPA-NLuc-Biosensor-Retrovirus-TD138

    Plasmid
    #222876
    Purpose
    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). Mixed WT/V84R Glycophorin A (GPA) scaffold.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA(V84R)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    FRB chain: V84R in GPA
    Promoter
    MSCV LTR
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222876-plasmid-map-sequence-id-441621

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  19. DRD3-DuET

    Plasmid
    #213229
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    DRD3 (DRD3 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213229-plasmid-map-sequence-id-430895

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  20. PvLEA4_repeats_k3_26_mCh-SspB (pBS1075)

    Plasmid
    #185299
    Purpose
    Mammalian expression of sleeping chironomid protein PvLEA4_repeats_k3_26 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    pvLEA22mer_shuffle_3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    185299-plasmid-map-sequence-id-363926

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Showing: 2041 - 2060 of 2117 results