We narrowed to 26,839 results for: She

1. Rac1 in pAcG2T

   Plasmid

   #38164

   Depositor

   Nora Heisterkamp

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   polyhedron

   Available since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

2. Bcr in pAcG2T

   Plasmid

   #38163

   Depositor

   Nora Heisterkamp

   Insert

   BCR (BCR Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pDC10

   Plasmid

   #75077

   Purpose

   Produces Escherichia coli glycinamide ribonucleotide synthetase (H6EcPurD)

   Depositor

   JoAnne Stubbe, T. J. Kappock

   Insert

   phosphoribosylglycinamide synthetase (purD Escherichia coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   adds unique SacII site at Thr95-Ala96 by a silent…

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSBBi-RP CCS1

   Plasmid

   #139162

   Purpose

   SB-transposon with constitutive bi-directional promoter. One side contains coffee caffeine synthase 1 (CCS1); the other side contains RFP and puromycin resistance genes.

   Depositor

   Neal Devaraj

   Insert

   caffeine synthase 1 [Coffea arabica]
   

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBBi-GB CaXMT1

   Plasmid

   #139168

   Purpose

   SB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica xanthosine methyltransferase 1 (CaXMT1); the other side contains GFP and blasticidin resistance genes.

   Depositor

   Neal Devaraj

   Insert

   7-methylxanthosine synthase 1 [Coffea arabica]
   

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSB-Bleo Gal4 CCS1

   Plasmid

   #139189

   Purpose

   SB-transposon with constitutive bleomycin resistance and Gal4-inducible coffee caffeine synthase 1 (CCS1).

   Depositor

   Neal Devaraj

   Insert

   caffeine synthase 1 [Coffea arabica]
   

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Gal4-inducible CMV and STAT3

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-METTL14-WT

   Plasmid

   #141112

   Purpose

   Overexpression of WT METTL14 in mammalian cells.

   Depositor

   Jianjun Chen

   Article

   Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

   Insert

   HMETTL14 (METTL14 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-VTKS2-Dreadd-hM3Dq-HA-P2A-tBFP

   Plasmid

   #178707

   Purpose

   AAV vector for Cre-dependent transgene expression of Dreadd-hM3Dq-HA-P2A-tBFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Insert

   Dreadd-hM3Dq-HA-P2A-tBFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX4T2-Ste7-EE

   Plasmid

   #52681

   Purpose

   expression of constitutively active Ste7 kinase in bacteria

   Depositor

   Benjamin Turk

   Article

   Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

   Insert

   Ste7 (STE7 Budding Yeast)
   

   Use

   Tags

   GST and Myc

   Expression

   Bacterial

   Mutation

   S359E/T363E constitutively active

   Promoter

   tac

   Available since

   April 30, 2014

   Availability

   Academic Institutions and Nonprofits only

10. LENTICRISPR-GPI_sgRNA1

    Plasmid

    #201594

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    GPI (GPI Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOEM1_pCMV:bmp4-pPH:vsvged

    Plasmid

    #111157

    Purpose

    Baculovirus rescue vector, VSVGED pseudotype, hShh

    Depositor

    Elly Tanaka

    Article

    Wagner et al Dev Cell. 2017 Mar 27;40(6):608-617.e6.

    Insert

    hBmp4 (BMP4 Human)
    

    Use

    Baculoviral rescue shuttle vector

    Tags

    Expression

    Insect and Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LENTICRISPR-GPI_sgRNA2

    Plasmid

    #201595

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    GPI (GPI Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LentiCRISPRv2-mCherry

    Plasmid

    #99154

    Purpose

    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

    Depositor

    Agata Smogorzewska

    Inserts

    Cas9
    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Pet28a_Human_H3.1

    Plasmid

    #42631

    Depositor

    Joe Landry

    Insert

    Human H3.1 (H3C1 Human)
    

    Use

    Tags

    Untagged

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 25, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. Pet28a_Human_H4

    Plasmid

    #42633

    Depositor

    Joe Landry

    Insert

    Human H4 (H4C1 Human)
    

    Use

    Tags

    Untagged

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 25, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pFLAG-6a-Ataxin3Q22

    Plasmid

    #22126

    Depositor

    Henry Paulson

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-GFAP-hM3D(Gq)-mCherry

    Plasmid

    #50478

    Purpose

    Gq-coupled hM3D DREADD fused with mCherry under the control of GFAP promoter

    Depositor

    Bryan Roth

    Has Service

    AAV5

    Insert

    hM3D(Gq)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    GFAP

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-hSyn-DIO-EGFP

    Plasmid

    #50457

    Purpose

    Double floxed EGFP under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, and AAV8

    Insert

    EGFP
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FLAG-hMYOD1 (#794)

    Plasmid

    #78329

    Purpose

    Overexpression of human MYOD1 ORF with a N-terminal FLAG tag

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    MYOD1 (MYOD1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pQC V5 MCS IRES G418

    Plasmid

    #110345

    Purpose

    γ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Geneticin selection.

    Depositor

    Sandra Martha Gomes Dias

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAG-eCas9-GFP-U6-gRNA

    Plasmid

    #79145

    Purpose

    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells

    Depositor

    Jizhong Zou

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-FLEX-tdTomato

    Plasmid

    #28306

    Depositor

    Edward Boyden

    Has Service

    AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    tdTomato
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLVX ALIX-GFP WT

    Plasmid

    #138582

    Purpose

    Expresses fluorescent ALIX in mammalian cells

    Depositor

    Wesley Sundquist

    Insert

    ALIX (PDCD6IP Human)
    

    Use

    Lentiviral

    Tags

    GFP and h30

    Expression

    Mammalian

    Mutation

    Promoter

    TREtight

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV14_Fibrillarin

    Plasmid

    #73061

    Purpose

    expression clone for human Fibrillarin with C-terminal 3xFLAG-tag

    Depositor

    Stefan Stamm

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. OmpA3-PNGaseF-TEV-His6

    Plasmid

    #114274

    Purpose

    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.

    Depositor

    Martin Caffrey

    Insert

    Peptide: N-glycosidase F
    

    Use

    Tags

    OmpA3 leader sequence (for periplasmic localisati…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pML104-KanMx4

    Plasmid

    #83476

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    KanMX4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pE-SUMO CRYGS

    Plasmid

    #80753

    Purpose

    Expression of human gamma S-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYGS (CRYGS Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pC1-CMV-mito-DAAO

    Plasmid

    #141132

    Purpose

    Expresses DAAO with mitochondrial localization signal in mammalian cells

    Depositor

    Vsevolod Belousov

    Insert

    mito-DAAO
    

    Use

    Tags

    2 mitochondrial localization signals and 7xHis

    Expression

    Mammalian

    Mutation

    deleted DAAO amino acids 367-368

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSL-MS2-12X

    Plasmid

    #27119

    Depositor

    Robert Singer

    Article

    Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

    Insert

    MS2 binding sites (12)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-hSyn-DIO-mCherry

    Plasmid

    #50459

    Purpose

    Double floxed mCherry under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    mCherry
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CAG-AausFP1-Z1

    Plasmid

    #135047

    Purpose

    AausFP1 - bright green fluorescent protein expression from A. australis under CAG promoter in minimal backbone plasmid.

    Depositor

    Jordan Green

    Insert

    AausFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG Promoter

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPB-US-ECasE

    Plasmid

    #83961

    Purpose

    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.

    Depositor

    Eleanor Chen

    Insert

    ERT2-spCas9-ERT2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    please see depositor comments below

    Promoter

    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Halo-TOMM20-N-10

    Plasmid

    #123284

    Purpose

    Localization: Outer Mitochondrial Membrane

    Depositor

    Kevin McGowan

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    HaloTag7

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBABEpuro-CRAF

    Plasmid

    #51124

    Purpose

    mammalian expression of wt CRAF

    Depositor

    Matthew Meyerson

    Insert

    CRAF (RAF1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro

    Plasmid

    #73037

    Purpose

    Express Nluc and copGFP dual reporter

    Depositor

    Kazuhiro Oka

    Insert

    Nluc-P2A-copGFP-T2A-Puro
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCR4-24XMS2SL-stable

    Plasmid

    #31865

    Depositor

    Robert Singer

    Article

    Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

    Insert

    24xMS2 Stem Loops - Stable
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV CTG 700x

    Plasmid

    #63087

    Purpose

    AdenoAssociated Virus plasmid containing expanded repeats--CTG 700x that is the mutation found in Myotonic Dystrophic type 1

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    CTG REPEATS
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CTG 700x that is the mutation found in Myotonic D…

    Promoter

    CMV

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSBBi-Pur GFP-TM

    Plasmid

    #139185

    Purpose

    SB-transposon with constitutive bi-directional promoter. One side contains transmembrane-anchored GFP; the other side contains the puromycin resistance gene.

    Depositor

    Neal Devaraj

    Insert

    GFP-TM
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    fused to membrane anchor

    Promoter

    EF1α

    Available since

    March 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pENN.AAV.CamKII.GCaMP6f.WPRE.SV40

    Plasmid

    #100834

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from CamKII promoter

    Depositor

    James M. Wilson

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    CamKII

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSBBi-Pur LaG17-synNotch-TetRVP64

    Plasmid

    #158133

    Purpose

    Constitutive expression of anti-GFP nanobody (LaG-17) synNotch TetRVP64 receptor in a pSBBi-Pur Sleeping Beauty backbone

    Depositor

    Neal Devaraj

    Insert

    LaG17-synNotch-TetRVP64
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge