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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 11,169 results for: ENA

Showing: 5151 - 5200 of 11169 results

  5. LSB-hsa-miR-29b-3p

    Plasmid
    #103394
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-29b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-29b-3p target
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103394-plasmid-map-sequence-id-195837

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  6. pCK301

    Plasmid
    #87767
    Purpose
    E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacI
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Insert
    PrhaBAD-sfGFP
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87767-plasmid-map-sequence-id-177279

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  15. pPPC011

    Plasmid
    #171143
    Purpose
    Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Insert
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    Sp.pCas9, BBa_J23107
    Available since
    Oct. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    171143-plasmid-map-sequence-id-345216

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  16. pMOD_C2515

    Plasmid
    #91083
    Purpose
    Module C, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    AtU6
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91083-plasmid-map-sequence-id-182047

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  18. pMOD_C2518

    Plasmid
    #91087
    Purpose
    Module C, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    TaU6
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91087-plasmid-map-sequence-id-182052

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  21. LSB-hsa-miR-941

    Plasmid
    #103757
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-941 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-941 target
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103757-plasmid-map-sequence-id-196562

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  22. AA145

    Plasmid
    #215937
    Purpose
    Fragmid fragment: (guide cassette) guide expression; Chen F+E trRNA (preferred)
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215937-plasmid-map-sequence-id-431395

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  30. SpIMPDH

    Plasmid
    #206980
    Purpose
    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.
    Depositor
    Vern Schramm
    Article
    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210
    Insert
    SpIMPDH (guaB Streptococcus pyogenes)
    Use
    Tags
    His tag (with TEV cleavage site)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    206980-plasmid-map-sequence-id-410397

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  31. pXW109Hg(RS-RinA-E11)2

    Plasmid
    #123151
    Purpose
    Mercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J109, PmerT, PhrpLE, PrinA
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123151-plasmid-map-sequence-id-244015

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  33. LSB-hsa-miR-181a-5p

    Plasmid
    #103276
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-181a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-181a-5p target
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103276-plasmid-map-sequence-id-195601

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  35. pBF_HsPepT1

    Plasmid
    #73943
    Purpose
    Xenopus oocyte expression vector containing the cDNA of human PepT1 with a c terminal flag tag
    Depositor
    Simon Newstead
    Article
    Beale et al Structure. 2015 Oct 6;23(10):1889-99. do
    Insert
    PepT1 (SLC15A1 Human)
    Use
    Make rna for xenopus oocyte injection
    Tags
    Flag
    Expression
    Mutation
    Promoter
    beta globin
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  39. pBF_HsPepT2

    Plasmid
    #73944
    Purpose
    Xenopus oocyte expression vector containing the cDNA of human PepT2 with a c terminal flag tag
    Depositor
    Simon Newstead
    Article
    Beale et al Structure. 2015 Oct 6;23(10):1889-99. do
    Insert
    PepT2 (SLC15A2 Human)
    Use
    Make rna for xenopus oocyte injection
    Tags
    Flag
    Expression
    Mutation
    Promoter
    beta globin
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  45. NSP14-Flag

    Plasmid
    #210346
    Purpose
    Expresses SARS-CoV-2 NSP14 fused with Flag in mammalian cells
    Depositor
    Michele Pagano
    Article
    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1
    Insert
    SARS-CoV-2 NSP14 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    210346-plasmid-map-sequence-id-418213

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  46. pPKm-248

    Plasmid
    #90504
    Purpose
    Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tPCYA - IRES - MTS - tHO1 - P2A - MTS - tFD - P2A - MTS - tFNR
    Depositor
    Todd Coleman
    Article
    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717
    Insert
    mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    90504-plasmid-map-sequence-id-204704

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  47. pCas9-beta-mmr

    Plasmid
    #169241
    Purpose
    Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations
    Depositor
    Brian Pfleger
    Article
    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:
    Inserts
    cas9
    beta
    mutL-E36K
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Changed glutamic acid 36 to lysine
    Promoter
    Available since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    169241-plasmid-map-sequence-id-333575

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Showing: 5151 - 5200 of 11169 results