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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,144 results for: LEA

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Showing: 2061 - 2080 of 16144 results

  2. pMM0647

    Plasmid
    #129008
    Purpose
    Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    KanR-ColE1 URA 5'-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1--pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
    Expression
    Yeast
    Available Since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129008-plasmid-map-sequence-id-259450

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  4. RFN_EGFP_47

    Plasmid
    #60071
    Purpose
    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease
    Depositor
    Keith Joung
    Article
    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Insert
    pair of multiplex gRNAs targeting EGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. TVMV-HRV-3C Proteases-mCherry

    Plasmid
    #243796
    Purpose
    Encodes for mammalian expression of TVMV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.
    Depositor
    Cole DeForest
    Article
    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/
    Insert
    TVMV and HRV-3C proteases
    Expression
    Mammalian
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    243796-plasmid-map-sequence-id-489527

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  11. mGreenLantern-[(A OR S) AND T] OR (C AND V)-SpyTag

    Plasmid
    #243789
    Purpose
    Expresses multifunctional construct enabling the [AND/(OR)]OR(AND)-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9), sortase eSrtA(4S9), TEV, HRV-3C, TEV and TVMV proteases. Construct contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/
    Insert
    mGreenLantern
    Tags
    6x His tag and SsrA
    Expression
    Bacterial
    Available Since
    Oct. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    243789-plasmid-map-sequence-id-486131

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  12. TVMV-TEV Proteases-mCherry

    Plasmid
    #243797
    Purpose
    Encodes for mammalian expression of TVMV and TEV protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.
    Depositor
    Cole DeForest
    Article
    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/
    Insert
    TVMV and TEV proteases
    Expression
    Mammalian
    Available Since
    Oct. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    243797-plasmid-map-sequence-id-486448

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  14. SP_C3

    Plasmid
    #228159
    Purpose
    Plasmid contains the secretion signal peptide sequence of the yeast (strain ATCC 204508 / S288c) PIR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_Q03178_PIR1_YEAST:0-18
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228159-plasmid-map-sequence-id-451652

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  20. PX458_3XHA_dFnCas9_KRAB_T2A_eGFP

    Plasmid
    #200389
    Purpose
    3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression
    Depositor
    Debojyoti Chakraborty
    Insert
    PX458_3XHA_dFnCas9_KRAB_eGFP
    Use
    CRISPR
    Tags
    3xHA-NLS and SV40NLS-KRAB-T2A-EGFP
    Expression
    Mammalian
    Promoter
    CBH
    Available Since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    200389-plasmid-map-sequence-id-407168

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Showing: 2061 - 2080 of 16144 results