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pCAG-1BPNLS-Cas9-1BPNLS

Plasmid

#87108

Purpose

BPNLS-Cas9-BPNLS expression plasmid

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

1BPNLS-Cas9-1BPNLS

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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monomer-NN

Plasmid

#27965

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-monomer-NN

Expression

Bacterial

Available Since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only
pLAMP1-miRFP703

Plasmid

#79998

Purpose

LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

LAMP1 (Lamp1 Rat)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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HA.PKD.deltaPH

Plasmid

#10811

Depositor

Alex Toker

Article

Storz et al J Biol Chem. 2003 May 16. 278(20):17969-

Insert

PKD (PRKD1 Human)

Tags

HA

Expression

Mammalian

Mutation

PH domain deleted (amino acids 417-545); F94I, V4…

Promoter

CMV

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pENTR1A-FKBP12DD C-term (w350-2)

Plasmid

#17413

Purpose

Gateway entry vector for Degron, C-term

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

FKBP12DD (L106P)

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFPC2-FmnIso1b

Plasmid

#19320

Depositor

Philip Leder

Article

Zhou et al Exp Cell Res. 2006 Apr 15. 312(7):1119-2

Insert

formin1 iso1b (Fmn1 Mouse)

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pISH-Venus

Plasmid

#15865

Depositor

Peter Mombaerts

Article

Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

Insert

Venus

Available Since

May 6, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA-I-PhoCl-HCVp

Plasmid

#87697

Purpose

Express I-PhoCl-HCVp with N-term EBFP2 in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

I-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)

Tags

EBFP

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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PM-FRB-CFP

Plasmid

#67517

Purpose

FRB conjugated plasma membrane targeting sequences from GAP43 (MLCCMRRTKQVEKNDDQKI)

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

mTOR (MTOR Human)

Tags

CFP, HA, and PM tethering sequence (MLCCMRRTKQVEK…

Expression

Mammalian

Mutation

FRB domain only (residues 2019–2114)

Promoter

CMV

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
pLenti-sgRNA-lib 2.0

Plasmid

#89638

Purpose

sgRNA expression in mammalian cells after Gateway cloning

Depositor

Wensheng Wei

Article

Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCHARGE3

Plasmid

#24658

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

Turbo

Expression

Bacterial

Available Since

June 9, 2010

Availability

Academic Institutions and Nonprofits only

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pFA6a-3HA-His3MX6

Plasmid

#41600

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Available Since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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pETMutS

Plasmid

#13245

Depositor

Peggy Hsieh

Article

Biswas et al J Biol Chem 1996 Mar 1;271(9):5040-8.

Insert

MutS

Expression

Bacterial

Mutation

None

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-hAIM2

Plasmid

#51542

Purpose

Express GFP-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-TC10

Plasmid

#23232

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

TC10 (RHOQ Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pENTR4-FKBP12DD N-term A (B3-1)

Plasmid

#17414

Purpose

Gateway entry vector for Degron, N-term, frame A

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

FKBP12DD (L106P)

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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PL-452 N-EGFP

Plasmid

#19173

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Oct;36(18):e114.

Insert

EGFP

Use

Cre/Lox

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pCDNAHygro(+)LAMP2B

Plasmid

#86029

Purpose

Expresses LAMP2B in Mammalian cells

Depositor

Janice Blum

Article

Zhou et al Immunity. 2005 May;22(5):571-81.

Insert

Lysosome-associated membrane protein 2b (LAMP2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pYES2-Ub-G76V-GFP

Plasmid

#11954

Depositor

Nico Dantuma

Article

Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

Insert

Ub-G76V-GFP

Expression

Yeast

Mutation

Ubiquitin fused to N-terminus of GFP. Glycine 76 …

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-FLEX-tdTomato-WPRE

Plasmid

#51505

Purpose

Can be used to generate AAV virus that will express tdTomato in the presence of Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

tdTomato

Use

AAV

Promoter

phSyn1

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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