We narrowed to 3,145 results for: N-EGFP

1. pCMV-enCas12a-ABE8e-V106W

   Plasmid

   #193653

   Purpose

   Expression of base editor enCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLb-enCas12a-TadA*8e-V106W, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dLbCas12a-D156R/G532R/K538R, TadA*8e-V106W

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-enCas12a-A3A-Y130F

   Plasmid

   #193652

   Purpose

   Expression of base editor enCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLb-enCas12a-hA3A-Y130F, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-Y130F

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-RVR-A3A-Y130F

   Plasmid

   #193658

   Purpose

   Expression of base editor RVR-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   RVR-dLbCas12a-hA3A-Y130F, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dLbCas12a-G532R/K538V/Y542R, hAPOBEC3A-Y130F

   Promoter

   CMV

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-RVR-A3A-N57G

   Plasmid

   #193657

   Purpose

   Expression of base editor RVR-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   RVR-dLbCas12a-hA3A-N57G, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dLbCas12a-G532R/K538V/Y542R, hAPOBEC3A-N57G

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDGB1alpha2_KanR_BastaR_Rb7_GFP

   Plasmid

   #186427

   Purpose

   optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)

   Depositor

   Lukas Fischer

   Article

   Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

   Inserts

   KanR
   BastaR
   Rb7
   eGFP
   

   Use

   Synthetic Biology; Binary vector for escherichia …

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   CaMV 35S and Pnos

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-dCas12a-ABE8e-V106W

   Plasmid

   #193646

   Purpose

   Expression of base editor dCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-TadA*8e-V106W, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   TadA*8e-V106W

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-RR-ABE8e-V106W

   Plasmid

   #193656

   Purpose

   Expression of base editor RR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-TadA*8e-V106W, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dLbCas12a-G532R/K595R, TadA*8e-V106W

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-RVR-ABE8e-V106W

   Plasmid

   #193659

   Purpose

   Expression of base editor RVR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   RVR-dLbCas12a-TadA*8e-V106W, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dLbCas12a-G532R/K538V/Y542R, TadA*8e-V106W

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-dCas12a-A3A-N57G

   Plasmid

   #193639

   Purpose

   Expression of base editor dCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-hA3A-N57G, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hAPOBEC3A-N57G

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-dCas12a-A3A-Y130F

    Plasmid

    #193640

    Purpose

    Expression of base editor dCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-hA3A-Y130F, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hAPOBEC3A-Y130F

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. retro-gfp-puro vector

    Plasmid

    #58249

    Purpose

    Retroviral expression vector encoding bicistronic EGFP and Puromycin cassette

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

    Insert

    EGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. px458-CCND1

    Plasmid

    #172630

    Purpose

    Expresses a gRNA against CCND1 and Cas9 from S. pyogenes with 2A-EGFP

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    gRNA against CCND1 (CCND1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-enCas12a-A3A-N57G

    Plasmid

    #193651

    Purpose

    Expression of base editor enCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLb-enCas12a-hA3A-N57G, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-N57G

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-RR-A3A-N57G

    Plasmid

    #193654

    Purpose

    Expression of base editor RR-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    RR-dLbCas12a-hA3A-N57G, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dLbCas12a-G532R/K595R, hAPOBEC3A-N57G

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-RR-A3A-Y130F

    Plasmid

    #193655

    Purpose

    Expression of base editor RR-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    RR-dLbCas12a-hA3A-Y130F, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dLbCas12a-G532R/K595R, hAPOBEC3A-Y130F

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. px458-AMBRA1 #3

    Plasmid

    #172607

    Purpose

    Expresses gRNA #3 against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    gRNA against AMBRA1 (AMBRA1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SiriusGFP

    Plasmid

    #123201

    Purpose

    We have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.

    Depositor

    Joseph Santos-Sacchi

    Article

    Zhong et al J Neurosci Methods. 2019 Feb 1;313:68-76

    Insert

    SiriusGFP
    

    Use

    Mouse Targeting

    Tags

    N/A

    Expression

    Bacterial and Mammalian

    Mutation

    eGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBa.GFP-KIF1Bbeta tail 387-1770

    Plasmid

    #134620

    Purpose

    Mammalian expression of mouse KIF1Bbeta 387-1170

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF1Bbeta (Kif1b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 386 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBa.GFP-KIF1Balpha tail 387-1150

    Plasmid

    #134619

    Purpose

    Mammalian expression of mouse KIF1Balpha 387-1150

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF1Balpha (Kif1b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 386 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEFEX2-A2aGH

    Plasmid

    #160544

    Purpose

    pYES2 derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

    Depositor

    Michelle O'Malley

    Article

    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

    Insert

    PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N/A

    Promoter

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge