We narrowed to 5,055 results for: cre encoding plasmid

1. pBOB-CAG-SARS-CoV2-Spike D614G-HA

   Plasmid

   #158761

   Purpose

   3rd Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein D614G high infectivity mutant with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao

   Depositor

   Gerald Pao

   Article

   Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.

   Insert

   SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Spike D614G

   Promoter

   CAG

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

   Plasmid

   #64218

   Purpose

   Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-BFP-P2A-E1B

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6

   Plasmid

   #64222

   Purpose

   Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry-P2A-E4orf6

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6

   Plasmid

   #64220

   Purpose

   Expression vector for sgRNA and Cas9 linked via T2A to BFP linked to the Ad4 E4orf6 gene via P2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-BFP-P2A-E4orf6

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)

   Plasmid

   #64217

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   hU6 promoter; BbsI sites for sgRNA
   H1 promoter; BamHI site for shRNA
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh, H1, and U6

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)

   Plasmid

   #114090

   Purpose

   CAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tag

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized MbCas12a
   

   Use

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pECT3

   Plasmid

   #75450

   Purpose

   Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   pdt3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pECT5

   Plasmid

   #75451

   Purpose

   Plasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   pdt5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pECT1

   Plasmid

   #75446

   Purpose

   Plasmid that serves as the PCR template to create genomic pdt1 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   pdt1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pECT1B

    Plasmid

    #75447

    Purpose

    Plasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    pdt1B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pECT1D

    Plasmid

    #75448

    Purpose

    Plasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    pdt1D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pECT1E

    Plasmid

    #75449

    Purpose

    Plasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    pdt1E
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pECM1

    Plasmid

    #75445

    Purpose

    Plasmid that serves as the PCR template to create the genomic pdt1 insertions contained in the EPD library. Plasmid contains the R6K origin of replication so needs pir gene to replicate

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    pdt1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE

    Plasmid

    #202199

    Purpose

    Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombination

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Has Service

    AAV1 and AAV5

    Insert

    stChrimsonR, PdCO
    

    Use

    AAV and Cre/Lox

    Tags

    EGFP and Rho1D4

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE

    Plasmid

    #198516

    Purpose

    Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Cre-dependent recombination.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Has Service

    AAV1 and AAV5

    Insert

    PdCO
    

    Use

    AAV and Cre/Lox

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. MADR pDonor smFP-HA (dark) WPRE

    Plasmid

    #131007

    Purpose

    MADR donor plasmid for FlpO+Cre- mediated insertion of SM_FP-HA, a cytoplasmic fluorescent protein which includes multiple HA epitope tags

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    smFP-HA WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    HA - 10 total HA epitope tages

    Expression

    Mammalian

    Mutation

    "dark" variant with GGG fluorphore comp…

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. MADR pDonor smFP-V5 (dark) WPRE

    Plasmid

    #131006

    Purpose

    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-V5, a cytoplasmic fluorescent protein which includes multiple V5 epitope tags

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    smFP-V5 WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    V5 - 10 total of V5 epitope tag

    Expression

    Mutation

    "dark" variant with GGG fluorphore comp…

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pREPROII-attB

    Plasmid

    #86557

    Purpose

    Dox inducible expression of reprogramming factors (Klf4, Sox2, Oct4, & cMyc), integration via phiC31 attB sites, excision via Cre/loxP

    Depositor

    Ralf Kuehn

    Article

    Merkl et al PLoS One. 2013;8(1):e55170. doi: 10.1371

    Inserts

    Oct4 (Pou5f1 Mouse)
    cMyc (Myc Mouse)
    Sox2 (Sox2 Mouse)
    Kruppel-like factor 4 (Klf4 Mouse)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (AAV9)

    Viral Prep

    #105551-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (#105551). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 plasmid DNA. Expression of GFP-Cre from CamKII promoter. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    CaMKII

    Tags

    GFP

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

20. pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (AAV1)

    Viral Prep

    #105540-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (#105540). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. Expression of EGFP-Cre from hSyn promoter. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    Syn

    Tags

    EGFP

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only