Skip to main content
Addgene
Showing: 5251 - 5300 of 6656 results
  1. pHL 1277

    Plasmid
    #53017
    Purpose
    AmpR + PLtetO-1::RBS (st7) gfp::T1 terminator + PLlacO-1::RBS (st7) mCherry::Asp terminator + ColE1
    Depositor
    Inserts
    GFP
    mCherry
    Use
    Synthetic Biology
    Tags
    RBS(st7)
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only
  2. pHL 1278

    Plasmid
    #53018
    Purpose
    AmpR + PLtetO-1::RBS (st7) mCherry::T1 terminator + PLlacO-1::RBS (st7) gfp::Asp terminator + ColE1
    Depositor
    Inserts
    mCherry
    GFP
    Use
    Synthetic Biology
    Tags
    RBS(st7)
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only
  3. pHL 1032

    Plasmid
    #52997
    Purpose
    pLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Inserts
    RhyB sRNA
    ompC
    sodB
    Use
    Synthetic Biology
    Tags
    gfp and mCherry
    Expression
    Bacterial
    Mutation
    contains −56 to +141 bp relative to start codon a…
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  4. pHPHS926

    Plasmid
    #228237
    Purpose
    Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible β-globin NMD reporter with premature termination codon for integration into Bxb1 attP_GA landing pad
    Inserts
    mCherry
    Beta globin
    Use
    Tags
    3xFLAG, HA, and PuroR
    Expression
    Mammalian
    Mutation
    PTC39
    Promoter
    TRE3GV and none
    Available since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pRB133.2

    Plasmid
    #181950
    Purpose
    aTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirK
    Depositor
    Insert
    PhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 Synthetic, PhoP-Salmonella enterica subsp. enterica serovar Typhimurium)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    PhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pET24a-LaM4-2xTS

    Plasmid
    #162779
    Purpose
    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to two tyrosine sulfation (TS) motifs. LaM4-2xTS also contains a T7, HA, BAP and His6 epitope
    Depositor
    Insert
    anti-mCherry nanobody fused to two TS sites, T7, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, T7, and TS site from proCCK
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pLBM0070

    Plasmid
    #172822
    Purpose
    Level 2 module containing mCHERRY-nYFP HSP21-cYFP OEP7-mTRQ
    Depositor
    Insert
    [35S-P19][35S-CTPSSU-mCHERRY-3XFLAGnYFP][35S-CTPSSU-mHSP21-cYFPX3HA][OEP7_mTRQ]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pUC19-hsg(G:H)

    Plasmid
    #138259
    Purpose
    Expression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter system
    Depositor
    Insert
    mCherry targeting sgRNAs G and H
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. pHL 762

    Plasmid
    #52990
    Purpose
    pLtetO-1:MicC, pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp
    Depositor
    Inserts
    MicC sRNA
    sodB
    ompC
    Use
    Synthetic Biology
    Tags
    gfp and mCherry
    Expression
    Bacterial
    Mutation
    −56 to +141 bp and −81 to +36 bp
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  10. pHL 1035

    Plasmid
    #52999
    Purpose
    pLtetO-1:RhyB, pCon:fhlA::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Inserts
    RhyB sRNA
    fhlA
    sodB
    Use
    Synthetic Biology
    Tags
    gfp and mCherry
    Expression
    Bacterial
    Mutation
    contains −309 to +96 bp relative to start codon a…
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  11. pHL 1033

    Plasmid
    #52998
    Purpose
    pLtetO-1:RhyB, pCon:rpoS::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Inserts
    RhyB sRNA
    rpoS
    sodB
    Use
    Synthetic Biology
    Tags
    gfp and mCherry
    Expression
    Bacterial
    Mutation
    contains −150 and +30 bp relative to start codon …
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  12. pHL 1158

    Plasmid
    #53007
    Purpose
    pLtetO-1:RhyB, pCon:DsrA, pCon:ompC::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Inserts
    RhyB sRNA
    DsrA sRNA
    ompC
    sodB
    Use
    Synthetic Biology
    Tags
    gfp and mCherry
    Expression
    Bacterial
    Mutation
    contains -56 to +141 relative to start codon and …
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  13. pTSK450

    Plasmid
    #34552
    Depositor
    Insert
    MS2-CP-NLS
    Use
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Promoter
    Met25
    Available since
    Jan. 19, 2012
    Availability
    Academic Institutions and Nonprofits only
  14. pLenti.PGK.chFP.W

    Plasmid
    #51008
    Purpose
    Lentivector that expresses chFP from internal human PGK promoter
    Depositor
    Insert
    mChFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human PGK
    Available since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
  15. pRubiC-T2A-Cas9

    Plasmid
    #75347
    Purpose
    Ubiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
    Depositor
    Insert
    Cas9
    Use
    CRISPR and Retroviral
    Tags
    mCherry (via t2a)
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
  16. pUT_mNF-Cas13d-Triplex

    Plasmid
    #227709
    Purpose
    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Include a 3' UTR Triplex motif as a template backbone for MONARCH 2.0 system.
    Depositor
    Insert
    mCherry-P2A-RfxCas13d-Triplex
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-d2i
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
  17. RFP-(SIM)3

    Plasmid
    #127148
    Purpose
    Mammalian expression plasmid for mCherry-(SIM)3 client to assess SUMO-SIM scaffolds.
    Depositor
    Insert
    3 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
  18. pAS132

    Plasmid
    #211503
    Purpose
    AAV genome to express mCherry under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq
    Depositor
    Insert
    mCherry
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
  19. pPEPZ-sgRNAclone

    Plasmid
    #141090
    Purpose
    This vector is designed for efficient cloning of sgRNAs by Golden Gate assembly. The sgRNA insertion leads to replacement of mCherry, resulting in loss of red color of E. coli colony.
    Depositor
    Insert
    mCherry
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    p3
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
  20. LLP618_αGCN4-KRAB-CO

    Plasmid
    #211782
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to transcriptional repressor KRAB, with GFP selection
    Depositor
    Insert
    aGCN4-mCherry
    Use
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect mCherr…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
  21. pASPIre1

    Plasmid
    #154842
    Purpose
    Derivative of pSEVA291 carrying the bxb1 gene under the control of a rhamnose-inducible promoter and mCherry flanked by attB/P sites
    Depositor
    Insert
    Bxb1 controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Prha
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
  22. pAN2981

    Plasmid
    #127246
    Purpose
    Asymmetric degronSwitchA_t8 fused to mCherry with KeyA-BFP
    Depositor
    Insert
    pPGK-Key_A_e18-TagBFP-P2A-mCherry-asym_degronSwitch_A_t8-WPRE
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 5251 - 5300 of 6656 results