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T444T-GFP(NCO)

Plasmid

#183972

Purpose

Enhanced RNAi clone for C. elegans knockdown of non-codon-optimized GFP

Depositor

David Matus

Article

Palmisano et al CourseSource, 2022

Insert

GFP (non-codon-optimized)

Use

RNAi

Expression

Worm

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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rArc

Plasmid

#178206

Purpose

Ratiometric version of the genetically encoded voltage indicator ArcLight-Q239.

Depositor

Stefan Heinemann

Article

Ruhl et al Commun Biol. 2021 Oct 7;4(1):1164. doi:

Insert

rArc

Expression

Mammalian

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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peZinCh-2

Plasmid

#70008

Purpose

expresses eZinCh-2 Zn2+ FRET sensor in the cytosol of mammalian cells

Depositor

Maarten Merkx

Article

Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

Insert

eZinCh-2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pTK_Axud1_enh_394_Citrine

Plasmid

#130625

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Axud1_enh_394

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pFM699

Plasmid

#74636

Purpose

alias pFA6a-mCherry-natNT2 - vector for C-terminal tagging of yeast genes with mCherry and nourseothricin selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

mCherry

Expression

Yeast

Promoter

none

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-Chimeric_Butterfly_YR_1.0

Plasmid

#59801

Purpose

Genetically encoded fluorescent voltage indicator

Depositor

Thomas Knopfel

Article

Mishina et al Front Mol Neurosci. 2014 Sep 29;7:78. do

Insert

Yellow-Red Chimeric Butterfly Voltage Sensitive Fluorescent Protein

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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AiRT_RTctrl

Plasmid

#214070

Purpose

Fluorescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Expression

Mammalian

Mutation

Linker sequence present between RFP and GFP

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAG461-FRB-redNFAST

Plasmid

#160362

Purpose

Expresses FRB-redNFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

FRB-redNFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBbS9a-RFP

Plasmid

#126172

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23113

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pmKalama1-Nuc

Plasmid

#14894

Purpose

Mammalian expression of mKalama1 blue fluorescent protein with NLS

Depositor

Robert Campbell

Article

Ai et al Biochemistry. 2007 May 22;46(20):5904-10

Insert

pmKalama1-Nuc

Tags

NLS

Expression

Mammalian

Mutation

I178T

Promoter

CMV

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only
pJMK004

Plasmid

#34616

Purpose

Viral expression of Arch (D95N mutant with greater sensitivity but slower response) voltage indicator

Depositor

Adam Cohen

Article

Kralj et al Nat Methods. 2011 Nov 27. doi: 10.1038/n

Insert

Arch D95N

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Changed Aspartic Acid 95 to Asparagine

Promoter

CaMKII

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only

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GEV 2

Plasmid

#12616

Depositor

John Louis

Article

Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

Insert

GB1 domain of protein G as N terminal tag, with IgT antigen as insert (SV40gp6 SV40)

Tags

G protein and His

Expression

Bacterial

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
POM43-mTFP1

Plasmid

#69853

Purpose

N-terminal tagging with mTFP1 using LEU2 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mTFP1 minus start and stop codon

Expression

Yeast

Available Since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-Uch37C88A

Plasmid

#19416

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

Uch37 (UCHL5 Human)

Tags

2XHA

Expression

Mammalian

Mutation

C88A

Available Since

Jan. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pJethlpAmNeonGreenKm

Plasmid

#206816

Purpose

Template for hlpA-mneongreen amplification associated with kanamycin resistance gene

Depositor

Virginie Libante

Article

Mignolet et al Microlife. 2026 Feb 26;7:uqag006. doi: 1

Insert

hlpA

Use

Synthetic Biology

Tags

mNeonGreen

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG 3.1

Plasmid

#193802

Purpose

Protein production of ChemoG3.1 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG 3.1

Tags

10xHis

Expression

Bacterial

Mutation

EGFP: A206K, T225R

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAG460-FRB-greenNFAST

Plasmid

#160361

Purpose

Expresses FRB-greenNFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

FRB-greenNFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pIM691

Plasmid

#74641

Purpose

alias pFA6a-ymTagBFP-hphNT1 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and hygromycin selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

ymTagBFP

Expression

Yeast

Promoter

none

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pMito-iRFP720

Plasmid

#45464

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Tags

human cytochrome C oxidase subunit VIII

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pEYFP-p23

Plasmid

#108225

Purpose

Mammalian expression vector for EYFP fusion to human p23

Depositor

Didier Picard

Article

Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

Insert

EYFP-p23 (PTGES3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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