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  2. G12-CASE

    Plasmid
    #190714
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G12. Composed of the subunits G alpha 12 (GNA12) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al J Biol Chem. 2022 Aug 3:102328. doi: 10.
    Inserts
    GNB3 (GNB3 Human)
    GNGT9
    GNA12 (GNA12 Human)
    Use
    Luciferase
    Tags
    Nluc is inserted at A133/F134 within GNA12 and cp…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    190714-plasmid-map-sequence-id-379753

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  3. pFC34K LgBiT TK-Neo Human collagen type IV alpha 5 chain (COL4A5)

    Plasmid
    #229770
    Purpose
    Human Col4A5 tagged at C-terminal with LgBiT to be used with C-tagged SmBiT hCol4A3 and Flag-tagged hCol4A4 in Nanobit assay system
    Depositor
    Hirofumi Kai
    Article
    Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64
    Insert
    Human collagen type IV alpha 5 chain (COL4A5) (COL4A5 Human)
    Use
    Luciferase
    Tags
    LgBiT
    Expression
    Mutation
    Promoter
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    229770-plasmid-map-sequence-id-457425

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  4. pFC36K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

    Plasmid
    #229769
    Purpose
    Human Col4A3 tagged at C-terminal with SmBiT to be used with C-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system
    Depositor
    Hirofumi Kai
    Article
    Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64
    Insert
    Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)
    Use
    Luciferase
    Tags
    SmBiT
    Expression
    Mutation
    Promoter
    Available since
    Dec. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    229769-plasmid-map-sequence-id-456747

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  5. PGL4.11-COL1A1mSMAD

    Plasmid
    #230917
    Purpose
    Tested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.
    Depositor
    Avi Rosenberg
    Article
    Yang et al Lab Invest. 2024 May;104(5):100336. doi:
    Insert
    Human COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
    Use
    Luciferase
    Tags
    Luciferase-luc2p
    Expression
    Bacterial and Mammalian
    Mutation
    COL1A1 promoter with mutant potential SMAD bindin…
    Promoter
    COL1A1
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230917-plasmid-map-sequence-id-458138

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  6. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60226
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60226-plasmid-map-sequence-id-176980

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  7. pFN33K LgBiT TK-neo Human collagen type IV alpha 5 chain (COL4A5)

    Plasmid
    #229768
    Purpose
    Human Col4A5 tagged at N-terminal with LgBiT to be used with N-tagged SmBiT hCol4A3 and Flag-tagged hCol4A4 in Nanobit assay system
    Depositor
    Hirofumi Kai
    Article
    Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64
    Insert
    Human collagen type IV alpha 5 chain (COL4A5) (COL4A5 Human)
    Use
    Luciferase
    Tags
    LgBiT
    Expression
    Mutation
    Promoter
    Available since
    Dec. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    229768-plasmid-map-sequence-id-456746

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  12. pGL4.23 C5_15

    Plasmid
    #60322
    Purpose
    This plasmid contains a genomic fragment that is not active in human pancreatic islets, a minimal promoter and a luc2 gene. Can be used as negative control in reporter assays performed in human pancreatic islets.
    Depositor
    Jorge Ferrer
    Article
    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1
    Insert
    Non active element (nearest TSS PHLDA1) (PHLDA1 Human)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pFN35K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

    Plasmid
    #229767
    Purpose
    Human Col4A3 tagged at N-terminal with SmBiT to be used with N-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system
    Depositor
    Hirofumi Kai
    Article
    Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64
    Insert
    Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)
    Use
    Luciferase
    Tags
    SmBiT
    Expression
    Mutation
    See Depositor Comments
    Promoter
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229767-plasmid-map-sequence-id-457423

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  17. PGL4.11-COL1A1mTEAD

    Plasmid
    #230916
    Purpose
    Comparing with wild type COL1A1 promoter, tested whether mutant TEAD binding sites changed the promoter activity.
    Depositor
    Avi Rosenberg
    Article
    Yang et al Lab Invest. 2024 May;104(5):100336. doi:
    Insert
    Human COL1A1 promoter with mutant TEAD elements (COL1A1 Human)
    Use
    Luciferase
    Tags
    Luciferase- luc2p
    Expression
    Bacterial and Mammalian
    Mutation
    mutant potential TEAD bind elements AGGAAT to CTG…
    Promoter
    COL1A1
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230916-plasmid-map-sequence-id-460667

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  18. pGL4.23-hs737.1

    Plasmid
    #222473
    Purpose
    Luciferase vector containing the hs737 enhancer sequence (sequence containing variant 1). Variant 1 is chr10:128568698-A-G where the information is chromosome:positionInB38-RefAllele-AltAllele.
    Depositor
    Tychele Turner
    Article
    Padhi et al Hum Genomics. 2021 Jul 13;15(1):44. doi:
    Insert
    hs737 enhancer sequence (sequence containing variant 1) (LOC110120928 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Variant 1 is chr10:128568698-A-G where the inform…
    Promoter
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    222473-plasmid-map-sequence-id-459122

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  19. pGL4.23-hs737.3

    Plasmid
    #222475
    Purpose
    Luciferase vector containing the hs737 enhancer sequence (sequence containing variant 3). Variant 3 is chr10:128569437-G-A where the information is chromosome:positionInB38-RefAllele-AltAllele.
    Depositor
    Tychele Turner
    Article
    Padhi et al Hum Genomics. 2021 Jul 13;15(1):44. doi:
    Insert
    hs737 enhancer sequence (sequence containing variant 3) (LOC110120928 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Variant 3 is chr10:128569437-G-A where the inform…
    Promoter
    Available since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    222475-plasmid-map-sequence-id-442952

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  20. pGL4.23-hs737.2

    Plasmid
    #222474
    Purpose
    Luciferase vector containing the hs737 enhancer sequence (sequence containing variant 2). Variant 2 is chr10:128569418-T-C where the information is chromosome:positionInB38-RefAllele-AltAllele.
    Depositor
    Tychele Turner
    Article
    Padhi et al Hum Genomics. 2021 Jul 13;15(1):44. doi:
    Insert
    hs737 enhancer sequence (sequence containing variant 2) (LOC110120928 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Variant 2 is chr10:128569418-T-C where the inform…
    Promoter
    Available since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    222474-plasmid-map-sequence-id-442951

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Showing: 2101 - 2120 of 4772 results