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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,402 results for: 221

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Showing: 5351 - 5400 of 8402 results

  1. TFORF0472

    Plasmid
    #142636
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF221 (ZNF221 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142636-plasmid-map-sequence-id-397758

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  3. TFORF0128

    Plasmid
    #143203
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SP8 (SP8 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143203-plasmid-map-sequence-id-396083

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  4. pRetroX-Tight-Pur-Trim69 isoform E (codon optimized)

    Plasmid
    #199570
    Purpose
    Stable and dox. inducible expression of Trim69 isoform E (codon optimized) after retroviral-mediated gene transfer
    Depositor
    Andrea Cimarelli, Yuxin Song
    Article
    Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11
    Insert
    Trim69 isoform E (codon optimized) (TRIM69 Human)
    Use
    Retroviral; Allows for puromycin selection
    Tags
    Flag
    Mutation
    codon optimized
    Available Since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    199570-plasmid-map-sequence-id-392290

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  5. pRetroX-Tight-Pur-Trim69 isoform D (codon optimized)

    Plasmid
    #199569
    Purpose
    Stable and dox. inducible expression of Trim69 isoform D (codon optimized) after retroviral-mediated gene transfer
    Depositor
    Andrea Cimarelli, Yuxin Song
    Article
    Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11
    Insert
    Trim69 isoform D (codon optimized) (TRIM69 Human)
    Use
    Retroviral; Allows for puromycin selection
    Tags
    Flag
    Mutation
    codon optimized
    Available Since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    199569-plasmid-map-sequence-id-392289

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  6. pRetroX-Tight-Pur-Trim69 isoform C (codon optimized)

    Plasmid
    #199568
    Purpose
    Stable and dox. inducible expression of Trim69 isoform C (codon optimized) after retroviral-mediated gene transfer
    Depositor
    Andrea Cimarelli, Yuxin Song
    Article
    Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11
    Insert
    Trim69 isoform C (codon optimized) (TRIM69 Human)
    Use
    Retroviral; Allows for puromycin selection
    Tags
    Flag
    Mutation
    codon optimized
    Available Since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    199568-plasmid-map-sequence-id-392270

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  7. pHW582

    Plasmid
    #198819
    Purpose
    In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, higher baseline fluorescence
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::GCaMP7b-SL2-mKate2::let-858 3'UTR
    Expression
    Worm
    Available Since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    198819-plasmid-map-sequence-id-390987

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  9. pHW539

    Plasmid
    #198811
    Purpose
    Dominant negative form of Protein Kinase A (PKA). Lowers PKA activity in targeted neurons.
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::PKA(DN)-SL2-GFP::let-858 3'UTR
    Expression
    Worm
    Mutation
    G310D mutation to abolish the binding of cAMP of …
    Available Since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    198811-plasmid-map-sequence-id-391700

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  10. pD002

    Plasmid
    #198833
    Purpose
    Inhibition of synaptic vessel release due to disruption of synaptic vessel function by reactive oxygen species production.
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::miniSOG-103L-mCherry::let-858 3'UTR
    Expression
    Worm
    Available Since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    198833-plasmid-map-sequence-id-391659

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  11. pHW580

    Plasmid
    #198816
    Purpose
    In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, slow kinetics
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::GCaMP7s-SL2-mKate2::let-858 3'UTR
    Expression
    Worm
    Available Since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    198816-plasmid-map-sequence-id-390989

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  16. TFORF3209

    Plasmid
    #144685
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZBTB9 (ZBTB9 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144685-plasmid-map-sequence-id-350841

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  21. pUC19-U6-BsmBI_cassette-St1Cas9-sgRNA (KAC14)

    Plasmid
    #133791
    Purpose
    U6 promoter sgRNA entry vector used for all St1Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V1 sgRNA architecture from Carter et al. biorxiv 2018
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    St1Cas9 sgRNA entry vector
    Expression
    Mammalian
    Mutation
    V1 sgRNA architecture from Carter et al. biorxiv …
    Promoter
    U6
    Available Since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133791-plasmid-map-sequence-id-268588

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  22. pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)

    Plasmid
    #133792
    Purpose
    U6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    St3Cas9 sgRNA entry vector
    Expression
    Mammalian
    Mutation
    sgRNA architecture from Muller et al. Molecular T…
    Promoter
    U6
    Available Since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133792-plasmid-map-sequence-id-268589

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  42. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid
    #139998
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139998-plasmid-map-sequence-id-394975

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  49. GPR116-Tango

    Plasmid
    #66307
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR116 (ADGRF5 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 5351 - 5400 of 8402 results