We narrowed to 7,152 results for: Cad
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Plasmid#198833PurposeInhibition of synaptic vessel release due to disruption of synaptic vessel function by reactive oxygen species production.DepositorInsert15xUAS::miniSOG-103L-mCherry::let-858 3'UTR
ExpressionWormAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHW580
Plasmid#198816PurposeIn vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, slow kineticsDepositorInsert15xUAS::GCaMP7s-SL2-mKate2::let-858 3'UTR
ExpressionWormAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-E798AQ800A_M
Plasmid#146952PurposeInsect Expression of DmAGO1-E798AQ800ADepositorInsertDmAGO1-E798AQ800A (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations V315A and H…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-E798QQ800E_M
Plasmid#146953PurposeInsect Expression of DmAGO1-E798QQ800EDepositorInsertDmAGO1-E798QQ800E (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations V315A and H…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-H941A_M
Plasmid#146954PurposeInsect Expression of DmAGO1-H941ADepositorInsertDmAGO1-H941A (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations V315A and H…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-H941K_M
Plasmid#146955PurposeInsect Expression of DmAGO1-H941KDepositorInsertDmAGO1-H941K (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations V315A and H…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-H941Q_M
Plasmid#146956PurposeInsect Expression of DmAGO1-H941QDepositorInsertDmAGO1-H941Q (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations V315A and H…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-K690AK694A_M
Plasmid#146958PurposeInsect Expression of DmAGO1-K690AK694ADepositorInsertDmAGO1-K690AK694A (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations V315A and H…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-K694A_M
Plasmid#146959PurposeInsect Expression of DmAGO1-K694ADepositorInsertDmAGO1-K694A (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations V315A and H…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-R937A_M
Plasmid#146960PurposeInsect Expression of DmAGO1-R937ADepositorInsertDmAGO1-R937A (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations V315A and H…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-R937D_M
Plasmid#146961PurposeInsect Expression of DmAGO1-R937DDepositorInsertDmAGO1-R937D (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations V315A and H…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-D661E-siRNAres_J
Plasmid#146713PurposeInsect Expression of DmAGO1-D661E-siRNAresDepositorInsertDmAGO1-D661E-siRNAres (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations compared to…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-D661K-siRNAres_J
Plasmid#146714PurposeInsect Expression of DmAGO1-D661K-siRNAresDepositorInsertDmAGO1-D661K-siRNAres (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations compared to…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-K657E-siRNAres_J
Plasmid#146715PurposeInsect Expression of DmAGO1-K657E-siRNAresDepositorInsertDmAGO1-K657E-siRNAres (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations compared to…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-Y653L-siRNAres_J
Plasmid#146716PurposeInsect Expression of DmAGO1-Y653L-siRNAresDepositorInsertDmAGO1-Y653L-siRNAres (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations compared to…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsDCP1a_539-582 (MCSII)_G
Plasmid#146407PurposeBacterial Expression of HsDCP1a_539-582DepositorInsertHsDCP1a_539-582 (DCP1A Human)
ExpressionBacterialMutationone silent mutation T1716G compared to the sequen…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut2_G
Plasmid#146409PurposeBacterial Expression of HsDCP1a_539-582-mut2DepositorInsertHsDCP1a_539-582-mut2 (DCP1A Human)
ExpressionBacterialMutationone silent mutation T1716G compared to the sequen…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsDCP1a_539-582 (MCSII)-WT_G
Plasmid#146411PurposeBacterial Expression of HsDCP1a_539-582DepositorInsertHsDCP1a_539-582 (DCP1A Human)
ExpressionBacterialMutationone silent mutation T1716G compared to the sequen…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Dest-MBP-Nsp1
Plasmid#191483PurposeMammalian expression of MBP-tagged SARS-CoV-2 Nsp1DepositorInsertMBP-Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionMammalianAvailable SinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[R272A,K276A, R280A,K284A]
Plasmid#188599PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
TagsFLAG-MycExpressionInsectMutationR272A [CGG=> GCG], K276A [AAG=>GCG], R280A …Available SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-hFAM3B-HA
Plasmid#180145PurposeCoding sequence of human FAM3B pCS2 with C terminal HA tagDepositorAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPOLY_new
Plasmid#179276PurposeExpresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)DepositorInsertscellodextrin phosphorylase
cellobiose phosphorylase
sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
UseSynthetic BiologyTags6xHIS and Strep-Tag IIExpressionBacterialPromoterT7 lacOAvailable SinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBICI_high ori
Plasmid#179273PurposeExpresses CuCBP and BaSP in E. coli BL21(DE3)DepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialPromoterT7 lacOAvailable SinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDUBI
Plasmid#179274PurposeExpresses CcCDP monocistronically and CuCBP and BaSP bicistronicallyDepositorInsertscellodextrin phosphorylase
cellobiose phosphorylase
C8077_RS00435 sucrose phosphorylase [ Bifidobacterium adolescentis (C8077_RS00435 Bifidobacterium alodescentis)
UseSynthetic BiologyTags6xHIS and Strep-Tag IIExpressionBacterialMutationsucrose phosphorylasePromoterT7 lacOAvailable SinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-hFAM3B-FLAG
Plasmid#180146PurposeCoding sequence of human FAM3B in pCS2 with C terminal FLAG tagDepositorAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSJ982
Plasmid#176622PurposeExpress yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertUb-MPGLW-Fbp1
TagsUbiquitin N-terminal fusion and triple flag tag C…ExpressionYeastMutationChanged first 4 N-terminal amino acid residues of…PromoterTDH3tc3Available SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-K690A_M
Plasmid#146957PurposeInsect Expression of DmAGO1-K690ADepositorInsertDmAGO1-K690A (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations V315A and H…Available SinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEos2-dVHH[GFP] in pCSDEST
Plasmid#162870PurposeSuper-resolution of GFP in cultureDepositorInsertmEos2-dVHH[EGFP]
TagsmEos2ExpressionMammalianAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
mILK-R349A-EFGP
Plasmid#176898Purposemouse ILK with R349A point mutation cloned into pEGFP-N1DepositorInsertIntegrin-linked kinase (Ilk Mouse)
TagsEGFPExpressionMammalianMutationArginine 349 to AlaninePromoterCNVAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
mILK-R255A-EFGP
Plasmid#176897Purposemouse ILK with R255A point mutation cloned into pEGFP-N1DepositorInsertIntegrin-linked kinase (Ilk Mouse)
TagsEGFPExpressionMammalianMutationArginine 255 to AlaninePromoterCNVAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPodA11
Plasmid#162843PurposeExpression protein designed PodA10 from a TEV cleavable IPTG inducible plasmid with ampicillin resistanceDepositorInsertProtein Designed Pyocyanin demethylase
TagsTEV-cleavable 6x-His tagExpressionBacterialMutationA53N, I73T, A87V, M99V, A129T from WT PodAPromoterT7Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41sIII_GST-ALC1-K77R
Plasmid#174813PurposeBacterial expression of inactive ALC1 (K77R disrupts ATP binding)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41sIII_GST-ALC1-E175Q
Plasmid#174814PurposeBacterial expression of inactive ALC1 (E175Q disrupts ATP binding)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
deltaLoop-MIDAS
Plasmid#171605PurposeExpresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) without the loop region (a.a. 4458-4496) in bacteria.DepositorInsertMIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
TagsHis6 tag and SNAP tagExpressionBacterialMutationReplaced amino acids 4458-4496 with ASGSGS linkerAvailable SinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
KIF13B
Plasmid#163907PurposeFull length mouse KIF13B for purification from insect cellsDepositorAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
KIF13B 1-584
Plasmid#163908PurposeMotile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.DepositorAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3/flag-pBirA(1-K283)-FRB
Plasmid#154451Purposeexpresses flag-pBirA(1-K283)-FRB/biochemical assayDepositorInsertflag-pBirA(1-K283)-FRB
ExpressionMammalianPromoterT7 promoterAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-H2-Dd (Y84C/C121S/A139C)
Plasmid#136063PurposeMammalian expression of myc-tagged H2-Dd (Y84C/C121S/A139C mutant)DepositorInsertH2-Dd (H2-D1 Mouse)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationY84C/C121S/A139CPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-B15-VN
Plasmid#135514PurposeMammalian expression of VN-fused and myc-tagged HLA-B*15:01DepositorInsertHLA-B*15:01 (HLA-B Human)
TagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-B57-VN
Plasmid#135515PurposeMammalian expression of VN-fused and myc-tagged HLA-B*57:01DepositorInsertHLA-B*57:1 (HLA-B Human)
TagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-B8-VN
Plasmid#135511PurposeMammalian expression of VN-fused and myc-tagged HLA-B*08:01DepositorInsertHLA-B*08:01 (HLA-B Human)
TagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
PV259
Plasmid#132340PurposeCasD plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertCasD-AT-CBF1 (CBF1 Mustard Weed, CasD (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana))
TagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…Available SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV503
Plasmid#132342PurposeCasE plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertCasE-AT-CBF1 (CBF1 Mustard Weed, CasE (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana))
TagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…Available SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV504
Plasmid#132343PurposeCasA plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertCasA-AT-CBF1 (CBF1 CasA (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana), Mustard Weed)
TagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…Available SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
dre-cdk2 3'UTR sicheck2
Plasmid#121123Purposeluciferase assayDepositorInsertdre-cdk2 3'UTR (cdk2 Zebrafish)
ExpressionBacterialAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-HSF4b K294R
Plasmid#118359PurposeThis plasmid expresses amino-acids 200-493 of hHSF4b K294R mutant fused to the GAL-4 DNA binding domain. This construct cannot undergo sumoylation on K294.DepositorAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMGF130
Plasmid#96971PurposeS. pombe lem2 knockout cassette with hygro resistance geneDepositorInsertlem2 (lem2 Fission Yeast)
ExpressionBacterialMutationwhole ORF deletion and replaced with hygro resist…PromoterN/AAvailable SinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMGF169
Plasmid#96996Purposeish1 mCherry insertion cassette with G418 resistance geneDepositorInsertish1 (ish1 Fission Yeast)
TagsGST and mCherryExpressionBacterialMutationish1 C-terminal domain fused with mCherry + G418 …PromoterN/AAvailable SinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (205-222) (pGEX2T(TEV))
Plasmid#80633PurposeResidues 205-222 of CHMP4A, Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-60DepositorAvailable SinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
213-petDuet1-GBD-LUMP--cdc42
Plasmid#65973PurposeDual expression of GBD-LUMP and cdc42DepositorInsertsTagsGBD (GTPase binding domain from ACK1; residues 44…ExpressionBacterialMutationconstitutively active Cdc42 (Q61L)PromoterT7Available SinceJuly 13, 2015AvailabilityAcademic Institutions and Nonprofits only