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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 19,393 results for: IRE

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Showing: 5401 - 5450 of 19393 results

  22. sgSf3b1(T1)

    Plasmid
    #90424
    Purpose
    induces dsDNA break within mouse Sf3b1 gene for subsequent homology-directed recombination and coding sequence mutagenesis at codon 700
    Depositor
    Alex Minella
    Article
    Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519
    Insert
    mus Sf3b1 gRNA (Sf3b1 Mouse)
    Use
    CRISPR; Guiderna encoding for gene editing
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    90424-plasmid-map-sequence-id-190772

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  24. pNK091

    Plasmid
    #219769
    Purpose
    MoClo-compatible Level P vector carrying fungal bioluminescence system lacking luciferase gene
    Depositor
    Karen Sarkisyan
    Article
    Balakireva et al bioRxiv 2025.02.17.636591; doi: https://
    Insert
    pNos-KanR-ocsT | p35S-nnHispS-ocsT | pCmYLCV-npgA-ATPT | p35S-nnCPH-ocsT | pFMV-nnH3H-nosT
    Use
    Synthetic Biology
    Expression
    Plant
    Promoter
    pNos-KanR-ocsT | p35S-nnHispS-ocsT | pCmYLCV-npgA…
    Available Since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    219769-plasmid-map-sequence-id-438210

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  25. ATX3-D1

    Plasmid
    #184246
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain and UIM1 fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    Ataxin-3 Josephin domain and UIM1 (ATXN3 Human)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Premature stop codon N263X
    Promoter
    T7 promoter
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184246-plasmid-map-sequence-id-370314

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  30. p28-2iDMD-puro

    Plasmid
    #88895
    Purpose
    28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.
    Depositor
    Michele Calos
    Article
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Inserts
    Dystrophin (DMD Human)
    luciferase
    puromycin resistance enzyme
    mCherry
    Expression
    Mammalian
    Mutation
    codon optimized
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    88895-plasmid-map-sequence-id-451851

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  33. ATX3-13Q

    Plasmid
    #184248
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Synthetic, Human)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    C-terminal codon optimization for protein expres…
    Promoter
    T7 promoter
    Available Since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    184248-plasmid-map-sequence-id-370322

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  44. pL452(hygro)-Sf3b1-K700K

    Plasmid
    #90426
    Purpose
    selectable HDR vector to introduce silent mutation (position 700) in mus Sf3b1 gene (for use with pL452-Sf3b1-K700E and sgSf3b1(T1) gRNA vectors enabling generation of hemizygous K700E mutation)
    Depositor
    Alex Minella
    Article
    Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519
    Insert
    Sf3b1 - left and right homology arms (Sf3b1 Mouse)
    Use
    Homology-directed repair vector
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    90426-plasmid-map-sequence-id-190769

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  49. pCDNA3-Flag-SPRTN-Y117C

    Plasmid
    #110216
    Purpose
    Expression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.
    Depositor
    Ivana Marinovic-Terzic, Kristijan Ramadan
    Article
    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi
    Insert
    SPRTN (SPRTN Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Y117C, P296L (see depositor comments below)
    Available Since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    110216-plasmid-map-sequence-id-210941

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Showing: 5401 - 5450 of 19393 results