Addgene

We narrowed to 22,214 results for: Sele

Showing: 401 - 440 of 22214 results

Results per page:

Sort results by:

MR015-PHOX2BRightTALEN_NK

Plasmid

#49478

Purpose

Right TALEN for PHOX2B with NK

Depositor

Bruce Conklin

Article

Miyaoka et al Nat Methods. 2014 Mar;11(3):291-3. doi:

Insert

TALEN

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
MR015-PHOX2BLeftTALEN_NK

Plasmid

#49477

Purpose

Left TALEN for PHOX2B with NK

Depositor

Bruce Conklin

Article

Miyaoka et al Nat Methods. 2014 Mar;11(3):291-3. doi:

Insert

TALEN

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU295

Plasmid

#167694

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU294

Plasmid

#167689

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-FLEx-ER-TurboID

Plasmid

#160857

Purpose

Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

ER TurboID

Use

AAV

Tags

HA and V5

Expression

Mutation

Promoter

CAG

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-TBG-ER-TurboID

Plasmid

#149415

Purpose

Hepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

ER-TurboID

Use

AAV

Tags

HA and V5

Expression

Mutation

Promoter

TBG

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-tMCK-ER-TurboID

Plasmid

#149412

Purpose

Muscle-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

ER-TurboID

Use

AAV

Tags

HA and V5

Expression

Mutation

Promoter

tMCK

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
RelA cFlag pcDNA3

Plasmid

#20012

Depositor

Stephen Smale

Article

Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

Insert

RelA (Rela Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
p2CT-His-MBP-Lwa_Cas13a_WT

Plasmid

#91865

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LwaCas13a

Use

CRISPR

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-TBG-Cyto-TurboID

Plasmid

#149414

Purpose

Hepatocyte-specific expression of cytosolic TurboID, N-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

Cyto-TurboID

Use

AAV

Tags

V5

Expression

Mutation

Promoter

TBG

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pK18sB

Plasmid

#177838

Purpose

Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.

Depositor

Christopher Johnson, Gregg Beckham

Article

Jayakody et al Energy Environ. Sci., 2018,11, 1625-1638

Type

Empty backbone

Use

Bacterial gene replacement

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p2CT-His-MBP-Lbu_C2c2_WT

Plasmid

#83482

Purpose

Bacterial expression of WT L. buccalis C2c2 CRISPR effector.

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_WT

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAS9counter

Plasmid

#107192

Purpose

Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

Depositor

Steve Salipante

Article

Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

Inserts

Cas9
sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

prab17

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 V5 hHR23A

Plasmid

#13053

Depositor

Steven Grossman

Article

Brignone et al Oncogene. 2004 May 20. 23(23):4121-9.

Insert

hHR23A (RAD23A Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

R325K and L355P relative to NP_001257291.1

Promoter

Available since

Oct. 5, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Cerulean-Epac2-Venus

Plasmid

#138196

Purpose

FRET-based biosensor for monitoring Epac2 activation.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2-Venus (Rapgef4 Mouse, Synthetic)

Use

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU324

Plasmid

#167691

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU55

Plasmid

#167684

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::asCpf1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ151-HDR

Plasmid

#113631

Purpose

Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.

Depositor

Alex Kentsis

Inserts

Left homology arm
Puromycin resistance gene
Venus fluorescence gene
Thymidine kinase
Right homology arm

Use

Cre/Lox; Homology recombination selection vector

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1a promoter, EF1a promoter (T2A cleaving sequen…

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CMVSP6-nEGFP-SV40-PURO

Plasmid

#138364

Purpose

Lentiviral plasmid for nuclear EGFP expression driven by CMVSP6 and puromycin resistance under SV40 promoter for selection

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

nuclear EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU206

Plasmid

#167693

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::asCpf1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU322

Plasmid

#167683

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::asCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pKanB alpha

Plasmid

#223524

Purpose

Used for expression of secreted recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

Depositor

Geoff Lin-Cereghino

Article

Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Sept. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
p2CT-His-MBP-Lse_C2c2_WT

Plasmid

#83486

Purpose

Bacterial expression of WT L. seeligeri C2c2 CRISPR effector

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lse_C2c2_WT

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5 HA3-eDHFR-T2A-puro

Plasmid

#86395

Purpose

for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection

Depositor

David Bentley

Article

Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

Insert

HA3 eDHFR T2A puro

Use

CRISPR

Tags

HA3 eDHFR T2A puro

Expression

Mutation

R12Y, G67S and Y100I mutations in E coli DHFR

Promoter

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
HA.PKD.S738E/S742E

Plasmid

#10810

Depositor

Alex Toker

Article

Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

Insert

PKD (PRKD1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Activation loop active: S738E and S742E (bp 2212-…

Promoter

CMV

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pSN444

Plasmid

#108331

Purpose

Expresses Sat2 for Nourseothricin resistance selection of C. elegans

Depositor

Shinsuke Niwa

Article

Obinata et al PLoS One. 2018 May 9;13(5):e0197128. doi

Insert

Streptothricin acetyl transferase 2

Use

Tags

Expression

Worm

Mutation

Promoter

rps-27 promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3xTC20-alphaTubulin

Plasmid

#36327

Depositor

Amy Palmer

Article

Van Engelenburg et al Chembiochem. 2010 Mar 1;11(4):489-93.

Insert

alpha tubulin 1b with optimized tetracysteine peptides for ReAsH labeling (TUBA1B Human, Synthetic)

Use

Tags

3xTC20

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU53

Plasmid

#167679

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::spCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU54

Plasmid

#167680

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::spCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p2CT-His-MBP-Lsh_C2c2_WT

Plasmid

#83487

Purpose

Bacterial expression of WT L. shahii C2c2 CRISPR effector

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lsh_C2c2_WT

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU293

Plasmid

#167688

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDYSCKO-ADE1

Plasmid

#120264

Purpose

pDYSCKO canavanine co-selection vector with a guide targeting the ADE1 locus

Depositor

Christian Landry

Article

Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

Insert

pDYSCKO cassette-ADE1 targeting guide

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

SNR52

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB407

Plasmid

#68672

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Citrine

Expression

Plant

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU323

Plasmid

#167690

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::Cas9i

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB427

Plasmid

#68692

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

TagRFP

Expression

Plant

Mutation

Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB403

Plasmid

#68668

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

MpEF1alpha promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU292

Plasmid

#167687

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::Cas9i

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU61

Plasmid

#167692

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::asCpf1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-IRES-hygro-EGFP-Stx17

Plasmid

#174652

Purpose

Vector for overexpression of EGFP-tagged Stx17 with IRES for hygromycin selection.

Depositor

Lukas Kapitein

Article

Janssen et al J Cell Sci. 2021 Oct 1;134(19). pii: 271

Insert

EGFP-Stx17 (STX17 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

CMV

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU319

Plasmid

#167686

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::spCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 401 - 440 of 22214 results

Results per page:

Sort results by:

	More than 20 requests
	More than 50 requests
	More than 100 requests