We narrowed to 1,565 results for: MBP

1. pJL1-MBP-4xDQNAT

   Plasmid

   #128390

   Purpose

   In vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Maltose binding protein
   

   Use

   Tags

   4xDQNAT glycosylation tag and 6xHis tag

   Expression

   Bacterial

   Mutation

   A302T- please see depositor comment

   Promoter

   T7

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pelB-MBP-P13-ss

   Plasmid

   #137065

   Purpose

   E. coli expression clone (T7lac promoter) for mature P13 (aa 22-179) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
   

   Use

   Tags

   PelB signal peptide + His10 + maltose binding pro…

   Expression

   Bacterial

   Mutation

   mature P13: lacks the P13 signal peptide

   Promoter

   T7lac

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEVA221MBPc-AID-T7RNAP

   Plasmid

   #167975

   Purpose

   Expresses fusion protein MBPc-AID-T7RNAP in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

   Insert

   MBPc-AID-T7RNAP
   

   Use

   Tags

   MBPc and T7RNAP

   Expression

   Bacterial

   Mutation

   Promoter

   TetR-Ptet

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNIC-MBP-TEV-GG

   Plasmid

   #215794

   Purpose

   Cloning of target gene with N terminal MBP tag and TEV cleavage

   Depositor

   ASAP Discovery, Frank von Delft

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pET28a-MBP-(CBX1)3-WT

   Plasmid

   #180212

   Purpose

   Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

   Depositor

   Marcey Waters

   Article

   Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

   Insert

   CBX1 chromobox protein 1 x3 (CBX1 Human)
   

   Use

   Tags

   6xhis-tag and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-MBP-yATOM_mCherry

   Plasmid

   #209707

   Purpose

   Mammalian expression of y-ATOM biosensor for mCherry

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   MBP-yATOM_mCherry
   

   Use

   Tags

   MBP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMBP-LbCas12a H759A

   Plasmid

   #113518

   Purpose

   Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   LbCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   H759A

   Promoter

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. MBP-MEK1 S222TAG

   Plasmid

   #68302

   Purpose

   N-terminal MBP tagged human MEK1 gene S222TAG for bacterial expression

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   MBP-MEK1 S222TAG (MAP2K1 Synthetic, Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. K20‐scFv‐pSMBP2

   Plasmid

   #159940

   Purpose

   To generate baculovirus for insect cell expression of anti beta-1 integrin scFv K20

   Depositor

   Sandra Schmid

   Article

   Lakoduk et al Traffic. 2020 Sep;21(9):590-602. doi: 10

   Insert

   anti beta-1 integrin scFv
   

   Use

   Tags

   6xHis tag, FLAG tag, MBP fusion, Sortase A recogn…

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMBP-HIS2-Vps20_deltaC

    Plasmid

    #21491

    Depositor

    James Hurley

    Article

    Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

    Insert

    Vps20 Delta_C (VPS20 Budding Yeast)
    

    Use

    Tags

    6x His and MBP

    Expression

    Bacterial

    Mutation

    bp 511-663 deleted

    Promoter

    Available Since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

11. MBP-MEK1 S218TAG

    Plasmid

    #68301

    Purpose

    N-terminal MBP tagged human MEK1 gene S218TAG for bacterial expression

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    MBP-MEK1 S218TAG (MAP2K1 Synthetic, Human)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDB.His.MBP.3C NLRP1 CARD WT

    Plasmid

    #164008

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARD

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. MBP-GTSE1-His6

    Plasmid

    #69109

    Purpose

    For bacterial expression of recombinant human GTSE1 tagged with MBP and His.

    Depositor

    Stephen Royle

    Article

    Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

    Insert

    G2- and S-phase Expressed 1 (GTSE1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-MBP-yATOM_Ras

    Plasmid

    #209709

    Purpose

    Mammalian expression of y-ATOM biosensor for HRas

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    MBP-yATOM_Ras
    

    Use

    Tags

    MBP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMBP-parallel1-GK5_v2

    Plasmid

    #134288

    Purpose

    Expresses GK5 (isoform 2) in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    mouse Gk5 isoform 2; 534 amino acids

    Promoter

    T7

    Available Since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. 6xHis-MBP-huLb5Cas12a

    Plasmid

    #174684

    Purpose

    Expresses 6xHis-MBP tagged Lb5Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huLb5Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pelB-MBP-RevA-ss

    Plasmid

    #137064

    Purpose

    E. coli expression clone (T7lac promoter) for mature RevA (aa 25-160) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAF07416.1 (revA Borrelia burgdorferi B31)
    

    Use

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    lacks the RevA signal peptide and 5 residues (CKA…

    Promoter

    T7lac

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. 6xHis-MBP-huHkCas12a

    Plasmid

    #174683

    Purpose

    Expresses 6xHis-MBP tagged HkCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huHkCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pelB-MBP-DipA-ss

    Plasmid

    #137068

    Purpose

    E. coli expression clone (T7lac promoter) for mature DipA (aa 21-342) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
    

    Use

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature DipA: lacks the DipA signal peptide

    Promoter

    T7lac

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMBPcyto-TEV-BseRI

    Plasmid

    #157739

    Purpose

    empty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

    Depositor

    Debra Hansen

    Type

    Empty backbone

    Use

    Tags

    His10 + MBP (cytoplasmic expression; no signal se…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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