We narrowed to 2,475 results for: T2A

1. pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #113344

   Purpose

   Expresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteins

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   VP64-dCas9-p300core-T2A-MS2-p65-HSF1
   

   Use

   Piggybac

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-R1R2_EF1adCas9p300_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #113342

   Purpose

   Expresses dCas9-p300 and MS2-p65-HSF1 fusion proteins

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   dCas9-p300core-T2A-MS2-p65-HSF1
   

   Use

   Piggybac

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA5/FRT/TO_HaloTag9_T2A_EGFP

   Plasmid

   #169326

   Purpose

   Expression of HaloTag9 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   HaloTag9
   

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA

   Plasmid

   #112921

   Purpose

   Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   EF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUG-T2A-Cas9

   Plasmid

   #75346

   Purpose

   Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP (via t2a)

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT2A-EKAREV-NLS

   Plasmid

   #173856

   Purpose

   Encoding EKAREV-NLS.

   Depositor

   Michiyuki Matsuda

   Article

   Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

   Insert

   EKAREV-NLS
   

   Expression

   Mammalian

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTAPmyc-T2A-tagRFP

   Plasmid

   #140278

   Purpose

   Backbone for making a mammalian expression vector of the protein of interest

   Depositor

   Hirohide Saito

   Article

   Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

   Insert

   Empty backbone
   

   Use

   Synthetic Biology

   Tags

   myc-T2A-tagRFP

   Expression

   Mammalian

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pF3BGX-T2A-GAL4

   Plasmid

   #138392

   Purpose

   RMCE vector for generating C-terminal T2A-GAL4 fusion

   Depositor

   Shu Kondo

   Article

   Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

   Insert

   T2A-GAL4
   

   Expression

   Insect

   Available Since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. iBlueberry T2A mCherry

   Plasmid

   #72434

   Purpose

   Expressing of iBlueberry and mCherry in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Yu et al Protein Sci. 2016 Feb;25(2):308-15. doi:

   Insert

   iBlueberry T2A mCherry
   

   Expression

   Mammalian

   Mutation

   mutation E177Q in iBlueberry

   Available Since

   May 24, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2

    Plasmid

    #199484

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    Gal4vp16/4xnrUAS-mKate2
    

    Use

    CRISPR

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBEG R2-iCreT2ALuc-L3 #BGV208

    Plasmid

    #48992

    Purpose

    Supplies Cre recombinase and Luciferase and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Inserts

    cre recombinase
    Luciferase
    

    Use

    Luciferase; Gateway cloning vector

    Tags

    IRES (weak)

    Promoter

    Unknown

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

12. mT2A-td-sfGFP-FNF

    Plasmid

    #182339

    Purpose

    Recombineering cassette plasmid for T2A-td-sfGFP-FNF

    Depositor

    Mario Capecchi

    Insert

    T2A-td-sfGFP-FNF
    

    Use

    Mouse Targeting; Flp / frt

    Expression

    Mammalian

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pF3BGX-T2A-GAL80

    Plasmid

    #138396

    Purpose

    RMCE vector for generating C-terminal T2A-GAL80 fusion

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    T2A-GAL80
    

    Expression

    Insect

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRubiG-T2A-cre

    Plasmid

    #66693

    Purpose

    Recombine floxed alleles in newborn neurons

    Depositor

    Bryan Luikart

    Article

    Williams et al J Neurosci. 2015 Jan 21;35(3):943-59. do

    Insert

    GFP-T2A-cre
    

    Use

    Retroviral

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

15. ORCO-T2A-QF2

    Plasmid

    #162524

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Orco gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Orco-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
    

    Expression

    Insect

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRubiC-T2A-cre

    Plasmid

    #66692

    Purpose

    Recombine floxed alleles in newborn neurons

    Depositor

    Bryan Luikart

    Article

    Williams et al J Neurosci. 2015 Jan 21;35(3):943-59. do

    Insert

    mCherry-T2A-Cre
    

    Use

    Retroviral

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT2Apuro-TRE-synPCB2.0

    Plasmid

    #139481

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
    

    Use

    Tol2 transposon donor vector

    Expression

    Mammalian

    Promoter

    Tet responsive element

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Nanog-t2A-mCherry

    Plasmid

    #127541

    Purpose

    Expresses the transactivation domain of mouse Nanog and mCherry via t2A linker.

    Depositor

    Jennifer Mitchell

    Article

    Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

    Insert

    Nanog (Nanog Mouse)
    

    Expression

    Mammalian

    Mutation

    Codes for the last 110 a.a of Nanog

    Promoter

    CMV

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKF-P14MMP2AG-T2APuroR-5h

    Plasmid

    #128256

    Purpose

    Mammalian positive feedback gene circuit in a Flp-In expression system controlling the PuroR gene. Also called mPF-PuroR.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    rtTA-Advanced::P2A::EGFP::T2A::PuroR
    

    Use

    Synthetic Biology; Flp-in expression vector

    Expression

    Mammalian

    Promoter

    tight TRE promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. psBIG1a ActBT2ATetP2AP - mAGH

    Plasmid

    #229973

    Purpose

    Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and mAGNLS-T2A-Hygro

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    ActBT2ATetP2AP - mAGH
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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