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Showing: 201 - 220 of 503 results

  2. G protein alpha-o-R179C-EE

    Plasmid
    #55780
    Purpose
    This is a constitutively activated G protein alpha-o mutant containing an internal EE epitope.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    G protein alpha-o with constitutively activating R179C mutation (Gnao1 Rat)
    Use
    Tags
    EE epitope was introduced internally with D167E a…
    Expression
    Bacterial and Mammalian
    Mutation
    Arginine 179 was replaced with cysteine
    Promoter
    CMV
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    55780-plasmid-map-sequence-id-122021

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  4. Cer(1-158)-RGS7

    Plasmid
    #55760
    Purpose
    An amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    mCer(1-158)-RGS7 (RGS7 Human, Aequorea victoria)
    Use
    Tags
    Cer(1-158) was fused to the amino terminus of RGS…
    Expression
    Mammalian
    Mutation
    RGS7 was amplified via PCR, which added an N-term…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55760-plasmid-map-sequence-id-115314

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  13. pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE P1214R K1277E

    Plasmid
    #166811
    Purpose
    Mammalian cell expression vector for FLAG-tagged NLRP1. LL1193EE and P1214R mutations abolish DPP9 association, K1277E abolishes UPA-UPA contacts
    Depositor
    Hao Wu
    Article
    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586
    Insert
    NLRP1 (NLRP1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    LL1193EE, P1214R, K1277E
    Promoter
    CMV
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166811-plasmid-map-sequence-id-328312

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  14. G protein alpha-q-YFP

    Plasmid
    #55782
    Purpose
    This G protein alpha-q construct contains internal insertions of YFP and the EE epitope.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    alpha-q EE YFP (Gnaq Mouse)
    Use
    Tags
    The EE epitope was introduced into the alpha-q se…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    55782-plasmid-map-sequence-id-119295

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  15. pCMV-αIFNα-ab

    Plasmid
    #110799
    Purpose
    Expression and secretion of an scFv antibody (clone) 4EA1 against all mouse interferon α subtypes in mammalian cells
    Depositor
    Thomas Böldicke
    Article
    Bussow et al PLoS One. 2019 Apr 16;14(4):e0215062. do
    Insert
    antibody against mouse interferon alpha
    Use
    Tags
    ER signal peptide, His6 tag, and myc epitope tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    110799-plasmid-map-sequence-id-211397

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  16. CFP(1-158)-RGS7t

    Plasmid
    #55761
    Purpose
    An amino-terminal CFP Fragment was fused to residues 202-477 of RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    RGS7(202-477)-CFP(1-158) (RGS7 Human, Aequorea victoria)
    Use
    Tags
    CFP(1-158) was fused to the amino terminus of RGS…
    Expression
    Mammalian
    Mutation
    The RGS sequence amino terminal to the GGL domain…
    Promoter
    CMV
    Available since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    55761-plasmid-map-sequence-id-89566

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  17. pTA7001-DEST

    Plasmid
    #71745
    Purpose
    GATEWAY compatible derivative of plant binary vector pTA7001 from Nam Chua's laboratory at The Rockefeller University, Dexamethasone inducible
    Depositor
    Roger Innes
    Article
    Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    GAL4
    Available since
    Feb. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    71745-plasmid-map-sequence-id-136974

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Showing: 201 - 220 of 503 results