We narrowed to 5,634 results for: chia

1. pAcBac3-OMeYRS-8xYtR-EGFP-3TAA-40TGA-152TAG-TEV-3xUAA

   Plasmid

   #228787

   Purpose

   EcTyr aaRS/tRNA system for TAG suppression with EGFP containing three stop codons for triple suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTyrRS
   EcYtR
   EGFP***
   TEV protease
   

   Use

   Baculoviral

   Tags

   Expression

   Mammalian

   Mutation

   TAG suppressor

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAcBac3-pAAFRS-8xYtR-EGFP-3TAA- 40TGA-152TAG-TEV-3xUAA

   Plasmid

   #228788

   Purpose

   EcTyr aaRS/tRNA system for pAAF incorporation with EGFP containing three stop codons for triple suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTyrRS
   EcYtR
   EGFP***
   TEV protease
   

   Use

   Baculoviral

   Tags

   Expression

   Mammalian

   Mutation

   TAG suppressor

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPICZ A-SLC[∆2-83]-His+MFN2-Strep (SB259)

   Plasmid

   #227608

   Purpose

   Inducible coexpression of His-tagged SLC25A46 in its N-terminal region (∆2-83) and Strep-tagged MFN2 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Use

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   aa 2-83 deleted

   Promoter

   AOX1

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPICZ A-SLC-His+MFN2-Strep (SB255)

   Plasmid

   #227604

   Purpose

   Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Use

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Anti-MBP [N128A/4R]

   Plasmid

   #206531

   Purpose

   Mammalian Expression Plasmid of anti-Maltose binding protein (MBP) (E. coli). Derived from hybridoma N128A/4.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Maltose binding protein (MBP) (Escherichia coli) recombinant Mouse monoclonal antibody (malE Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Anti-Thioredoxin [N245/44R]

   Plasmid

   #206546

   Purpose

   Mammalian Expression Plasmid of anti-Thioredoxin (E. coli). Derived from hybridoma N245/44.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Thioredoxin (Escherichia coli) recombinant Mouse monoclonal antibody (trxA Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNB1-LOX

   Plasmid

   #154058

   Purpose

   Level 1, Position 3 Golden Gate vector. ZmUbi-5'UTR:loxP-GUS-nosT-loxP-NAM-B1-nosT

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Insert

   LoxP-flanked GUS and TtNAM-B1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   The TtNAM-B1 gene sequence was domesticated to re…

   Promoter

   ZmUbi

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLY9

   Plasmid

   #130907

   Purpose

   A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Inserts

   dcas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   

   Use

   Synthetic Biology

   Tags

   ASV tag and pspFΔHTH::λN22plus

   Expression

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Anderson promoter: J23106, PpspA-2G6, and Ptet

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLY59

   Plasmid

   #130927

   Purpose

   A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::MCP controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Inserts

   dcas9
   tetR
   sfgfp
   pspFΔHTH::MCP
   

   Use

   Synthetic Biology

   Tags

   ASV tag and MCP (MS2 coat protein)

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Anderson promoter: J23106, PpspA-2G6, and Ptet

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLY57

    Plasmid

    #130926

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA5B5 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA5B5, and Ptet

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLY56

    Plasmid

    #130925

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA4B4 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA4B4, and Ptet

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLY55

    Plasmid

    #130924

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA3B3, and Ptet

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLY53

    Plasmid

    #130911

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. N295A (EcN), M163A (EcI) MetNIQ in pBAD

    Plasmid

    #118261

    Purpose

    N295A Mutation of MetN and M163A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (N295A) (metN E. coli)
    MetI (M163A) (metI E. coli)
    MetQ (metQ E. coli)
    

    Use

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …

    Promoter

    None and araBAD Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. N295A (EcN), M107A (EcI) MetNIQ in pBAD

    Plasmid

    #118259

    Purpose

    N295A Mutation of MetN and M107A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (N295A) (metN E. coli)
    MetI (M107A) (metI E. coli)
    MetQ (metQ E. coli)
    

    Use

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …

    Promoter

    None and araBAD Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. N295A (EcN), F103A (EcI) MetNIQ in pBAD

    Plasmid

    #118258

    Purpose

    N295A Mutation of MetN and F103A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (N295A) (metN E. coli)
    MetI (F103A) (metI E. coli)
    MetQ (metQ E. coli)
    

    Use

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …

    Promoter

    None and araBAD Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-syn-flex-iGluSnFR4s-NGR-WPRE

    Plasmid

    #234441

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-NGR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-syn-flex-iGluSnFR4f-NGR-WPRE

    Plasmid

    #234442

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-NGR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-syn-FLP-FRT-iGluSnFR4s-NGR-WPRE

    Plasmid

    #234453

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-NGR
    

    Use

    AAV; Flp/frt

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CAG-flex-iGluSnFR4s-PDGFR-WPRE

    Plasmid

    #234447

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-PDGFR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-CAG-flex-iGluSnFR4f-PDGFR-WPRE

    Plasmid

    #234448

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-PDGFR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE

    Plasmid

    #234454

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-NGR
    

    Use

    AAV; Flp/frt

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-syn-flex-iGluSnFR4s-PDGFR-WPRE

    Plasmid

    #234439

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-PDGFR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-syn-flex-iGluSnFR4f-PDGFR-WPRE

    Plasmid

    #234440

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-PDGFR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA_FRT_SNCA-Avi_HygR-2A-BirA.dna(TO_deleted). (pEHA1448)

    Plasmid

    #209083

    Purpose

    SNCA (Avi tagged) expression along with BirA ligase

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Inserts

    SNCA (SNCA Human)
    BirA
    

    Use

    Tags

    Avi

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. piggyBac_rtTA (4th_Gen)_Gateway_IRES_NGN2-2A-PURO_Synapsin-BirA. (pEHA1618)

    Plasmid

    #209084

    Purpose

    Ngn2 mediated cortical neuron induction, gateway destination, Synapsin promoter BirA ligase expression

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Inserts

    NGN2 (Neurog2 Mouse)
    BirA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    hSyn

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLY70

    Plasmid

    #130948

    Purpose

    A CRISPR activation device with the necessary genes (dxcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter PrhaB), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dxcas9 (3.7)
    tetR
    rhaS
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9 for dCas9; Sy…

    Promoter

    Pcon, PpspA-LEA3B3, PrhaB, and Ptet

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDGB1alpha2_KanR_BastaR_Rb7_GFP

    Plasmid

    #186427

    Purpose

    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Inserts

    KanR
    BastaR
    Rb7
    eGFP
    

    Use

    Synthetic Biology; Binary vector for escherichia …

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    CaMV 35S and Pnos

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLY61

    Plasmid

    #130928

    Purpose

    A CRISPR activation device with the necessary genes (dxcas9 3.7 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dxcas9 (3.7)
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9 for dCas9; Sy…

    Promoter

    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPICZ A-SLC-His+MFN2[∆TM]-Strep (SB260)

    Plasmid

    #227609

    Purpose

    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Inserts

    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    

    Use

    Tags

    10xHis and Twin-StrepTag

    Expression

    Yeast

    Mutation

    contains aa 2-544, 651-757 only [TM deleted]

    Promoter

    AOX1

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pPICZ A-SLC(2-88)-MBP-His+MFN2-Strep (SB262)

    Plasmid

    #227611

    Purpose

    Inducible coexpression of His-tagged SLC25A46 truncated to its N-terminal region (2-88) fused to MBP and MFN2-Strep in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Inserts

    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    

    Use

    Tags

    MBP, 10xHis and Twin-StrepTag

    Expression

    Yeast

    Mutation

    contains aa 2-88 only

    Promoter

    AOX1

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)

    Plasmid

    #227610

    Purpose

    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Inserts

    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    

    Use

    Tags

    10xHis and Twin-StrepTag

    Expression

    Yeast

    Mutation

    contains aa 2-400, 706-757 [TM and HB2 deleted]

    Promoter

    AOX1

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196082

    Purpose

    (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Aminoglycoside phosphotransferase
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)

    Plasmid

    #209086

    Purpose

    Ngn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expression

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    BirA
    

    Use

    Tags

    AviTag

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    TRE4G and hSyn

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623)

    Plasmid

    #209085

    Purpose

    Ngn2 mediated cortical neuron induction, SNCA(wt) expression, Synapsin promoter BirA ligase expression

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    BirA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    TRE4G and hSyn

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Syn61

    Bacterial Strain

    #174513

    Purpose

    Recoded E. coli strain without TCG, TCA, or TAG codons in all open reading frames. Full sequence - GenBank: CP040347.1

    Depositor

    Jason W Chin

    Article

    Fredens et al Nature. 2019 May;569(7757):514-518. doi:

    Bacterial Resistance

    Streptomycin

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

37. BM28

    Bacterial Strain

    #201047

    Purpose

    MG1655-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures. Also carries a pBR325 plasmid carrying groESL called pOF39.

    Depositor

    Jeannette Winter

    Article

    McGuire et al BMC Genomics. 2023 Mar 27;24(1):154. doi

    Bacterial Resistance

    Ampicillin and kanamycin

    Available since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

38. S4197

    Bacterial Strain

    #200839

    Purpose

    wildtype strain MG1655 rph+, ilvG+, ΔlacZ

    Depositor

    Karin Schnetz

    Article

    Venkatesh et al J Bacteriol. 2010 Dec;192(24):6456-64. d

    Bacterial Resistance

    None

    Species

    Escherichia coli str. k-12 substr. mg1655

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

39. BM28 ΔlysU

    Bacterial Strain

    #201048

    Purpose

    MG1655-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures.

    Depositor

    Jeannette Winter

    Article

    McGuire et al BMC Genomics. 2023 Mar 27;24(1):154. doi

    Bacterial Resistance

    Kanamycin

    Available since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

40. KTD101(DE3)

    Bacterial Strain

    #138651

    Purpose

    KTD101(DE3) is a trigger factor deficient strain, which may increase protein secretion from Escherichia coli, and is compatible with expression from the T7 promoter

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

41. BA14

    Bacterial Strain

    #186997

    Purpose

    Deletion of the nuo operon

    Depositor

    Steven Vik

    Article

    Amarneh et al Biochim Biophys Acta. 2006 Dec;1757(12):

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

42. EH1

    Bacterial Strain

    #102804

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC ilvA genes deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

    Bacterial Resistance

    None

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

43. ATMY-C321

    Bacterial Strain

    #218774

    Purpose

    E. coli strain, genotype: C321.ΔA pUltraBR-MjY ΔtyrS tyrTV::tolC tyrU::GentR lambda::ZeoR

    Depositor

    Abhishek Chatterjee

    Article

    Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

    Bacterial Resistance

    Spectinomycin (50 ug/ml), gentamycin (10 ug/ml)

    Species

    Escherichia coli

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

44. RF17

    Bacterial Strain

    #102801

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE genes deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

    Bacterial Resistance

    None

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

45. RF18

    Bacterial Strain

    #102802

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE avtA genes deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

    Bacterial Resistance

    None

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

46. BL21∆serB

    Bacterial Strain

    #34929

    Depositor

    Jesse Rinehart, Dieter Söll

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Bacterial Resistance

    None

    Available since

    Feb. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

47. eScaf

    Bacterial Strain

    #220921

    Purpose

    cssDNA production strain

    Depositor

    Shawn Douglas

    Article

    Shen et al Nucleic Acids Res. 2024 Apr 24;52(7):409

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

48. Top10∆serB

    Bacterial Strain

    #34928

    Depositor

    Jesse Rinehart, Dieter Söll

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Bacterial Resistance

    None

    Available since

    Feb. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

49. RF15

    Bacterial Strain

    #102799

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB genes deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

    Bacterial Resistance

    None

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

50. RF16

    Bacterial Strain

    #102800

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB cysE genes deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

    Bacterial Resistance

    None

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only