We narrowed to 5,724 results for: Chia

1. pPLtetO-FtsZ

   Plasmid

   #89656

   Purpose

   Plasmid for titrating synthesis of FtsZ with doxycycline

   Depositor

   Uwe Sauer

   Article

   Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

   Insert

   GTP-binding tubulin-like cell division protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-Nkx2-1/Puro

   Plasmid

   #31271

   Depositor

   Tyler Jacks, Monte Winslow

   Article

   Winslow et al Nature. 2011 May 5. 473(7345):101-4.

   Insert

   Nkx2-1* (Nkx2-1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPBCAG-rtTAM2-IH

   Plasmid

   #140827

   Purpose

   Expresses rtTAM2-IRES-HygR in mammalian cells

   Depositor

   Hideyuki Okano

   Article

   Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825

   Inserts

   rtTAM2
   HygR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. BB1_23_dCAS9(3xFLAG_SV40NLS)

   Plasmid

   #161478

   Purpose

   BB1 encoding a FLAG tagged dCAS9 with a SV40 NLS

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Insert

   dCAS9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   FLAG Tag, SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. His-DERA

   Plasmid

   #203225

   Purpose

   For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

   Depositor

   Vern Schramm

   Article

   Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

   Insert

   deoxyribose aldolase
   

   Use

   Tags

   N-terminal TEV cleavable 6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAJM.969

   Plasmid

   #108537

   Purpose

   MphRAM + EryR + PMph-YFP Reporter. Ery sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   MphRAM + EryR + PMph-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   MphR: N94D

   Promoter

   Available since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2-QFGal4-SV40pA

   Plasmid

   #155126

   Purpose

   In vitro transcription of QFGal4 mRNA

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   QFGal4
   

   Use

   Expression in zebrafish

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC-4CL1

   Plasmid

   #35947

   Depositor

   Claudia Schmidt-dannert

   Article

   Watts et al BMC Biotechnol. 2006 Mar 21;6:22.

   Insert

   4-coumaroyl CoA ligase 1 (4CL1 Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLac-mod

   Available since

   Sept. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXint129

   Plasmid

   #178208

   Purpose

   Encodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.

   Depositor

   Gregory Phillips

   Article

   Platt et al Plasmid. 2000 Jan . 43(1):12-23.

   Insert

   xis excisionase, int integrase (xis, lambdap32 Bacteriophage lambda)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacI

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPM1001

    Plasmid

    #167548

    Purpose

    Encodes genes that confer expression of Vibrio cholerae O antigens of the Inaba serotype in E. coli K-12

    Depositor

    Renato Morona

    Article

    Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

    Insert

    Vibrio cholerae O antigens genes of the Inaba serotype
    

    Use

    Cosmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET15b T7pCONS sfGFP

    Plasmid

    #154467

    Purpose

    pET15b with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMARO

    Plasmid

    #178086

    Purpose

    Derivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.

    Depositor

    Sören Abel

    Article

    Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10

    Insert

    TcR (tetA/tetR)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Tetracycline resistance cassette is flanked by Ec…

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPLtetO-ClpX

    Plasmid

    #89657

    Purpose

    Plasmid for titrating synthesis of ClpX with doxycycline

    Depositor

    Uwe Sauer

    Article

    Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

    Insert

    clpX
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIIS18-BsaI

    Plasmid

    #128649

    Purpose

    Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

    Depositor

    Tohru Suzuki

    Article

    Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBDK

    Plasmid

    #135190

    Purpose

    Integration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    KanR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cat

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGBW-m4046955

    Plasmid

    #145616

    Purpose

    Bacterial Expression plasmid for RNA-dependent RNA polymerase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    RNA-dependent RNA polymerase (Nsp12) (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pET-NtLNtC60αC20C60β

    Plasmid

    #160874

    Purpose

    Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcL, cpn60α, cpn60β, cpn20
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcL: pBAD, chaperones: T7

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNAS1B split mCherry N-link/link-C

    Plasmid

    #61965

    Purpose

    Negative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein)

    Depositor

    Lynne Regan

    Article

    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

    Inserts

    N-mCherry-link
    link-C-mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD and PLtetO

    Available since

    Feb. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXylA-agrCA-I

    Plasmid

    #53437

    Purpose

    xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    agrCA locus
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Bacterial

    Mutation

    "MVQTS" sequence added to the N-terminu…

    Promoter

    PxylA

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCNA

    Plasmid

    #135191

    Purpose

    Arabinose inducible expression of lamba-Red recombinase proteins and I-CreI.

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    I-CreI lamba Red
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    araBAD

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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