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pLenti CMV/TO Neo DEST (685-3)

Plasmid

#17292

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Hygro DEST H2B-mRuby2

Plasmid

#90236

Purpose

Lentiviral vector to express H2B-mRuby2 under PGK promoter (With Hygromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

mRuby2

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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mRFP-LAP2β

Plasmid

#228029

Purpose

lentiviral construct for mRFP-LAP2β expression

Depositor

David Pellman

Article

Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

Insert

LAP2b (Tmpo Rat)

Use

Lentiviral

Tags

mRFP

Expression

Mammalian

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLJC2-MTHFD1-3xFLAG

Plasmid

#217429

Purpose

Lentiviral overexpression of human MTHFD1

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

MTHFD1 (MTHFD1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-hMLH1dn-eGFP

Plasmid

#191104

Purpose

Lentiviral expression plasmid of hMLH1dn with P2A-eGFP reporter

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

hMLH1dn

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-GFP-HRP-TM_pLX304

Plasmid

#202021

Purpose

expresses GFP-HRP anchored to the extracellular side of the plasma membrane, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

Flag-GFP-HRP-TM

Use

Lentiviral

Tags

Flag and GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_PEmax-2A-BSD

Plasmid

#234071

Purpose

For lentiviral expression of PEmax with blasticidin resistance

Depositor

Greg Findlay

Article

Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

Insert

PEmax

Use

CRISPR and Lentiviral

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

EF-1α core promoter

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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TagBFP_A1aY1

Plasmid

#158752

Purpose

Lentiviral vector for expression of TagBFP_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

TagBFP-T_A1aY1

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Promoter

CAG (CMV enhancer and chicken beta actin promoter)

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-LGALS3

Plasmid

#175756

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS3

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Lentiviral

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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C1(1-29)-miniTurbo-V5_pLX304

Plasmid

#107176

Purpose

expresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vector

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Use

Lentiviral

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pRDA_887

Plasmid

#201164

Purpose

Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

Depositor

John Doench

Article

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Promoter

EFS

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Ftractin-mCherry

Plasmid

#85131

Purpose

lentiviral expression of an F-actin live-cell marker (mCherry)

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)

Use

Lentiviral

Tags

mCherry

Promoter

EF1a

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_Hinge Notch SNIPR (NRG1 JMD)

Plasmid

#188379

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a NRG1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_NRG1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS

Plasmid

#199349

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP-SUMOEu

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO Zeo DEST (644-1)

Plasmid

#17294

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Zeo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pHAGE-FAM134C-GFP

Plasmid

#201927

Purpose

For lentiviral transduction of FAM134C-GFP

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

FAM134C (RETREG3 Human)

Use

Lentiviral

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shSMAD4 #2

Plasmid

#83091

Purpose

Lentiviral shRNA vector for inducible knockdown of human SMAD4

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shSMAD4 (SMAD4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pLC-Flag-CRBN-P2A-Hygro

Plasmid

#124303

Purpose

Lentiviral vector for expression of Flag tagged CRBN-P2A-Hygro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

CRBN (CRBN Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD_IM

Plasmid

#84479

Purpose

Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Tet1CD_IM

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pHR_SFFv_4D5-High-CAR_RHL002

Plasmid

#164825

Purpose

Lentiviral vector for constitutive expression of AntiHER2 4D5-High CAR

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Insert

AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)

Use

Lentiviral

Promoter

SFFV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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