We narrowed to 2,568 results for: gem

1. pTC223

   Plasmid

   #70019

   Purpose

   Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7

   Depositor

   Daniel Voytas

   Article

   Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

   Insert

   Nuclease (Cas9/sgRNA) + Donor + GVR
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBES1-LacOx50-Hyg

   Plasmid

   #210261

   Purpose

   Integrates 50xLacO repeats upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Insert

   50xLacO repeats derived from: PMID: 12864855.
   

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTnos (GB0037)

   Plasmid

   #68188

   Purpose

   Provides the Agrobacterium tumefaciens Nopaline synthase terminator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Nos terminator
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pP35S (GB0030)

   Plasmid

   #68163

   Purpose

   Provides the CaMV 35S Promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   CaMV 35S promoter
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFP.E227

   Plasmid

   #138249

   Purpose

   Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   mCherry
   Terminator
   mTagBFP
   Terminator
   gfp
   Terminator
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P17_up1691, P20_992, and iP16_3622

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-tag

   Plasmid

   #55182

   Purpose

   HA-tag

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Insert

   HA tag
   

   Use

   Synthetic Biology; Bacillus biobrick box

   Available Since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. BII-ChBtW-iresFUCCI

   Plasmid

   #133371

   Purpose

   Piggybac vector for constitutive expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   Clover-Geminin-IRES-mKO2-Cdt1
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBW101ParsR-gfp

   Plasmid

   #78636

   Purpose

   pSB3K3 carrying J101-rbs32-arsR-t-ParsR-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

   Insert

   J101-rbs32-arsR-t-ParsR-30gfp-t
   

   Use

   Synthetic Biology

   Promoter

   see insert

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pBW103ParsR-Amp30C

   Plasmid

   #78638

   Purpose

   pBW100ParsR carrying 30hrpR-30hrpS-t-hrpL-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

   Insert

   30hrpR-30hrpS-t-hrpL-30gfp-t
   

   Use

   Synthetic Biology

   Promoter

   see insert

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFP.R267

    Plasmid

    #138179

    Purpose

    Expresses the split mCherry reporter interrupted by the gp41-1 intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(SGY).gp41-1.(SSS)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P(araBAD) and iPC

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFP.R377

    Plasmid

    #138247

    Purpose

    Expresses the split mCherry reporter interrupted by the Mja-KlbA intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(HDG).Mja-KlbA*.(CSG)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P(araBAD) and iPC

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. placZα-tdMCP

    Plasmid

    #191132

    Purpose

    Expression of tdMCP from a lacZα inducible promoter

    Depositor

    Ariel Lindner

    Article

    Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

    Insert

    dMCP_1
    

    Use

    Synthetic Biology

    Promoter

    lac-inducible promoter

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. PROM5_MpEF1a

    Plasmid

    #136113

    Purpose

    Strong promoter from MpEF1a, that drives expression everywhere in Mp gemmae but stronger in the notches

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM5_MpEF1a Mapoly0024s0116
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pKL004

    Plasmid

    #98920

    Purpose

    Constitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Insert

    GCaMP6f tethered to mRuby
    

    Tags

    His and mRuby3

    Expression

    Bacterial

    Promoter

    BBa_J23118 - Strong Constitutive

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLI-2xFKBP-GFP(1463000)

    Plasmid

    #85789

    Purpose

    P. falciparum integration plasmid to fuse endogenous genes with tags using SLI

    Depositor

    Tobias Spielmann

    Article

    Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PF3D7146300 targeting region, 2xFKBP-GFP-2TA-Neo-R
    

    Use

    P. falciparum integration

    Tags

    2xFKBP, GFP

    Promoter

    none

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLI-N-sandwich-loxP(K13)

    Plasmid

    #85793

    Purpose

    P. falciparum integration plasmid for N-terminal gene fusions (double 2xFKBP) and placing loxP sites using SLI

    Depositor

    Tobias Spielmann

    Article

    Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    Kelch13
    

    Use

    Cre/Lox; P. falciparum genomic integration

    Tags

    double 2xFKBP, GFP

    Promoter

    none

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLI-sandwich(0807600)

    Plasmid

    #85790

    Purpose

    P. falciparum integration plasmid to fuse endogenous genes with tags (double 2xFKBP tag) using SLI

    Depositor

    Tobias Spielmann

    Article

    Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PF3D0807600 targeting region, 2xFKBP-GFP-2xFKBP-2TA-Neo-R
    

    Use

    P. falciparum genomic integration

    Tags

    2xFKBP, GFP

    Promoter

    none

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFP.R265

    Plasmid

    #138167

    Purpose

    Expresses the split mCherry reporter interrupted by the SspDnaB M86 artificially mini-intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(VDA).M86.(SDL)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P(araBAD) and iPC

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGUS (GB0208)

    Plasmid

    #68203

    Purpose

    Provides the CDS of the β-glucuronidase gene as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    GUS coding region
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pISH-Barx1

    Plasmid

    #84306

    Purpose

    Use to synthesize riboprobes for in situ hybridization (ISH) probe for Barx1. Not for gene expression.

    Depositor

    Peter Mombaerts

    Article

    Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

    Insert

    Barx1 (Barx1 Mouse)
    

    Available Since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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