165,839 results

1. pCFJ420 - Peft-3::GFP::H2B (ubiquitous)

   Plasmid

   #34877

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

   Insert

   Peft-3 GFP H2B tbb-2 UTR
   

   Use

   Worm targeting

   Tags

   Expression

   Mutation

   S65C

   Promoter

   eef-1A.1 (eft-3)

   Available Since

   Feb. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGGDestTol2LC-5sgRNA

   Plasmid

   #64243

   Purpose

   destination vector for five U6x:sgRNA cassettes

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-hM3D(Gq)-mCherry (AAV8)

   Viral Prep

   #50474-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-hSyn-hM3D(Gq)-mCherry (#50474). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM3D(Gq)-mCherry plasmid DNA. hSyn-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   Syn

   Tags

   mCherry

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pCAG-hCas9

   Plasmid

   #51142

   Purpose

   Expresses hCas9 under the CAG promoter for CRISPR

   Depositor

   Izuho Hatada

   Article

   Horii et al Int J Mol Sci. 2013 Sep 30;14(10):19774-

   Insert

   hCas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pLEGFP-Y705F-STAT3

   Plasmid

   #71445

   Purpose

   Retroviral expression of Y705F-STAT3. Please note that this plasmid contains Y705F-STAT3 tagged with FLAG, not GFP.

   Depositor

   George Stark

   Article

   Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

   Insert

   STAT3 (STAT3 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mutation

   Y705F

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

6. CL20_mEGFP_NUP98_KDM5A

   Plasmid

   #205872

   Purpose

   Express mEGFP-tagged fusion protein, NUP98_KDM5A from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   NUP98_KDM5A (NUP98, KDM5A Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTetOFF SNCA-A53T

   Plasmid

   #215372

   Purpose

   All-in-one lentiviral construct for the dox repressible expression of the A53T mutant of alpha-synuclein.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   SNCA (SNCA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Ala53Thr

   Promoter

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-HA-Ubiquitin-KO

   Plasmid

   #17603

   Purpose

   Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   KO: all lysines mutated to arginines

   Promoter

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-mCh-Cry2WT

   Plasmid

   #101221

   Purpose

   Blue-light dependent optogenetic protein Cry2

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

   Insert

   Cry2PHR
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.synP.DIO.EGFP.WPRE.hGH (AAV9)

    Viral Prep

    #100043-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV.synP.DIO.EGFP.WPRE.hGH (#100043). In addition to the viral particles, you will also receive purified pAAV.synP.DIO.EGFP.WPRE.hGH plasmid DNA. Synapsin-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ian Wickersham

    Promoter

    Syn

    Tags

    EGFP (Cre-dependent)

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

11. GW1-Peredox-mCitrine

    Plasmid

    #32386

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    Peredox-mCitrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFR L858R

    Plasmid

    #11012

    Purpose

    Retroviral construct for expressing human EGFR with L858R mutation in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR L858R (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    L858R (tumor-derived)

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. p10xUAS-luc2

    Plasmid

    #173006

    Purpose

    firefly luciferase reporter vector with 10x clustered UAS binding sites

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    10xUAS-luc2
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-FLEX-tdTomato

    Plasmid

    #28306

    Depositor

    Edward Boyden

    Has Service

    AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    tdTomato
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid

    #50464

    Purpose

    Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promote

    Depositor

    Bryan Roth

    Insert

    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF1α-Cre-2A-mScarlet

    Plasmid

    #207745

    Purpose

    Expression of the Cre recombinase linked to a mScarlet fluorescent marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-mScarlet
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pcDNA3.1(+) Drp1

    Plasmid

    #34706

    Depositor

    David Chan

    Insert

    Drp1 (Dnm1l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-Cas9-GFP

    Plasmid

    #86145

    Purpose

    Lentiviral vector expressing Cas9-GFP

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    Cas9-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pfmh-hCry1

    Plasmid

    #25843

    Depositor

    Aziz Sancar

    Insert

    human Cryptochrome 1 (CRY1 Human)
    

    Use

    Tags

    flag, his, and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 9, 2010

    Availability

    Academic Institutions and Nonprofits only

20. hCathepsin L

    Plasmid

    #11250

    Purpose

    Mammalian expression of human cathepsin L

    Depositor

    Hyeryun Choe

    Article

    Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

    Has Service

    Cloning Grade DNA

    Insert

    cathepsin L (CTSL Human)
    

    Use

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 25, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pLVpuro-CMV-N-3xFLAG

    Plasmid

    #123223

    Purpose

    N-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pGal4BD-CRY2

    Plasmid

    #28243

    Purpose

    Expresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activation

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    Gal4BD

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLminP_Luc2P_RE59

    Plasmid

    #90404

    Purpose

    STAT3 - Sis-inducible element (SIE) gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    STAT3

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pEB2-mScarlet-I

    Plasmid

    #104007

    Purpose

    Plasmid encoding mScarlet-I

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mScarlet-I
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pGL3-EF1a-i53

    Plasmid

    #216243

    Purpose

    Express i53 in mammalian cells as a HDR enhancer

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    i53
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pDUAL CD81 (GFP)

    Plasmid

    #86980

    Purpose

    Lentiviral expression construct encoding CD81 and GFP from separate promoters

    Depositor

    Joe Grove

    Insert

    CD81 (CD81 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV/hPGK

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. pEE082 FoMV

    Plasmid

    #234811

    Purpose

    To clone guide RNA (gRNA) along with its scaffold in to the Foxtail Mosaic Virus Vector (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. pLV_3xFLAG-ATP6V0A3-mScarlet

    Plasmid

    #228867

    Purpose

    Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG and C-terminal mScarlet

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V0A3 (TCIRG1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG and mScarlet

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCE059-SiT-Cas12a-[Activ]

    Plasmid

    #128136

    Purpose

    Express both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcript

    Depositor

    Randall Platt

    Article

    Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

    Insert

    AsCas12a-[Activ]-Triplex
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSL1765 (pSPIN-R, R*MmeI)

    Plasmid

    #160734

    Purpose

    Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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